Sökning: onr:"swepub:oai:research.chalmers.se:ea9c25d5-a2f5-4941-8acd-f064be7206a8" >
Using Tetracysteine...
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye to Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis
-
- Ng, Janice S.W. (författare)
- University Of Cambridge
-
- Hanspal, Maya A. (författare)
- University Of Cambridge
-
- Matharu, Naunehal S. (författare)
- University Of Cambridge
-
visa fler...
-
- Barros, Teresa P. (författare)
- University Of Cambridge
-
- Esbjörner Winters, Elin, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Wilson, Mark R. (författare)
- University of Wollongong
-
- Yerbury, Justin J. (författare)
- University of Wollongong
-
- Dobson, C.M. (författare)
- University Of Cambridge
-
- Kumita, J.R. (författare)
- University Of Cambridge
-
visa färre...
-
(creator_code:org_t)
- 2019-09-06
- 2019
- Engelska.
-
Ingår i: Biochemistry. - : American Chemical Society (ACS). - 1520-4995 .- 0006-2960. ; 58:39, s. 4086-4095
- Relaterad länk:
-
https://research.cha... (primary) (free)
-
visa fler...
-
https://pubs.acs.org...
-
https://research.cha...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal lobar degeneration (FTLD). Wild type TDP-43 inclusions are a pathological hallmark of >95% of patients with sporadic ALS and of the majority of familial ALS cases, and they are also found in a significant proportion of FTLD cases. ALS is the most common form of motor neuron disease, characterized by progressive weakness and muscular wasting, and typically leads to death within a few years of diagnosis. To determine how the translocation and misfolding of TDP-43 contribute to ALS pathogenicity, it is crucial to define the dynamic behavior of this protein within the cellular environment. It is therefore necessary to develop cell models that allow the location of the protein to be defined. We report the use of TDP-43 with a tetracysteine tag for visualization using fluorogenic biarsenical compounds and show that this model displays features of ALS observed in other cell models. We also demonstrate that this labeling procedure enables live-cell imaging of the translocation of the protein from the nucleus into the cytosol.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas