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Adaptive Laboratory...
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Zhang, JingTianjin University
(författare)
Adaptive Laboratory Evolution of Halomonas bluephagenesis Enhances Acetate Tolerance and Utilization to Produce Poly(3-hydroxybutyrate)
- Artikel/kapitelEngelska2022
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2022-05-08
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MDPI AG,2022
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electronicrdacarrier
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LIBRIS-ID:oai:research.chalmers.se:ec588cbb-70fc-472d-b8ad-cdc32c1fd684
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https://research.chalmers.se/publication/530745URI
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https://doi.org/10.3390/molecules27093022DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
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Acetate is a promising economical and sustainable carbon source for bioproduction, but it is also a known cell-growth inhibitor. In this study, adaptive laboratory evolution (ALE) with acetate as selective pressure was applied to Halomonas bluephagenesis TD1.0, a fast-growing and contamination-resistant halophilic bacterium that naturally accumulates poly(3-hydroxybutyrate) (PHB). After 71 transfers, the evolved strain, B71, was isolated, which not only showed better fitness (in terms of tolerance and utilization rate) to high concentrations of acetate but also produced a higher PHB titer compared with the parental strain TD1.0. Subsequently, overexpression of acetyl-CoA synthetase (ACS) in B71 resulted in a further increase in acetate utilization but a decrease in PHB production. Through whole-genome resequencing, it was speculated that genetic mutations (single-nucleotide variation (SNV) in phaB, mdh, and the upstream of OmpA, and insertion of TolA) in B71 might contribute to its improved acetate adaptability and PHB production. Finally, in a 5 L bioreactor with intermittent feeding of acetic acid, B71 was able to produce 49.79 g/L PHB and 70.01 g/L dry cell mass, which were 147.2% and 82.32% higher than those of TD1.0, respectively. These results highlight that ALE provides a reliable method to harness H. bluephagenesis to metabolize acetate for the production of PHB or other high-value chemicals more efficiently.
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Jin, BiaoTianjin University
(författare)
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Fu, Jing,1985Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jingf
(författare)
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Wang, ZhiwenTianjin University
(författare)
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Chen, TaoTianjin University
(författare)
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Tianjin UniversityChalmers tekniska högskola
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Molecules: MDPI AG27:91420-30491420-3049
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