Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance

Kanagarajan, SelvarajuSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding (författare)

Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3 to 4). The PM altered the chlorophyll fluorescence (a/b ratio), maturity duration, and yield attributing traits. The yield loss ranged from 4.55% to 72.66%. After affirming the resistance level, the resistant mutant (RM) with minimum yield loss (scale:0) and the susceptible mutant (SM) with maximum loss (scale:4) were used for metabolomic analysis through GC-MS. PM infection induced expression of 66 metabolites representing 32 functional classes. The number of unique classes in RM and SM was 13 and 11, respectively, while eight were common. A fold change in the common metabolites indicated an enhanced accumulation of amine, alcohol, and ester in RM. Along with pathogen-induced defensive metabolites, RM produced silane and fluorene, whose biological significance in disease resistance is unknown. Though SM expressed defence-related bio-molecules, it failed to yield better.

Sveriges lantbruksuniversitetInstitutionen för växtförädling (creator_code:org_t)
Sveriges lantbruksuniversitet

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