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Effects of high mob...
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Ley, CeciliaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
(författare)
Effects of high mobility group box protein-1, interleukin-1 beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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2010-11-30
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Informa UK Limited,2011
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Taylor & Francis: STM, Behavioural Science and Public Health Titles,2024
Nummerbeteckningar
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LIBRIS-ID:oai:slubar.slu.se:46520
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https://res.slu.se/id/publ/46520URI
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https://doi.org/10.3109/03008207.2010.523803DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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The effects of high mobility group box protein (HMGB)-1, interleukin (IL)-1 beta, and IL-6 on equine articular chondrocytes were investigated, with emphasis on detecting differences between anatomical sites exposed to different loading in vivo, using three-dimensional (3D) cell cultures established with chondrocytes from dorsal radial facet (DRF, highly loaded) and palmar condyle (PC, less loaded) of the third carpal bone (C3). Expression of important genes involved in cartilage metabolism, presence of glycosaminoglycans and cartilage oligomeric matrix protein (COMP) in pellets, and concentrations of matrix metalloproteinase (MMP)-13 and aggrecan epitope CS 846 were evaluated. Compared to controls, IL-1 beta treatment increased gene expression of versican, matrix-degrading enzymes, and tissue inhibitor of metalloproteinase (TIMP)-1, and decreased aggrecan and collagen type I and type II expression. In addition, IL-1 beta-treated pellets showed decreased safranin O staining and increased COMP immunostaining and MMP-13 concentrations in culture supernatants. Effects of IL-6 and HMGB-1 on gene expression were variable, although upregulation of Sry-related high-mobility group box 9 (Sox9) was often present and statistically increased in HMGB-1-treated pellets. Response to cytokines rarely differed between DRF and PC pellets. Thus, site-associated cartilage deterioration in equine carpal osteoarthritis (OA) is not explained by topographically different responses to inflammatory mediators. Differences in gene expressions of structural matrix proteins in untreated DRF and PC pellets were noted in the youngest horses, which may indicate differences in the chondrocytes potential to produce matrix in vivo. Overall, a strong catabolic response was induced by IL-1 beta, whereas slight anabolic effects were induced by IL-6 and HMGB-1.
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Svala, EmiliaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)48811
(författare)
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Ekman, StinaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)47073
(författare)
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Skiöldebrand, EvaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)50022
(författare)
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Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Sammanhörande titlar
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Ingår i:Connective Tissue Research: Informa UK Limited52, s. 290-3000300-82071607-8438
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