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| 2. |
- Adlercreutz, P., et al.
(författare)
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Catalytic properties of α-chymotrypsin in organic media
- 1991
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Ingår i: Biomedica Biochimica Acta. - 0232-766X. ; 50:10-11, s. 55-60
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Tidskriftsartikel (refereegranskat)abstract
- α-Chymotrypsin was deposited on Celite and the resulting immobilized preparations were used to carry out peptide synthesis reactions in organic media with only small amounts of water present. The influence of different parts of the donor ester and acceptor nucleophile substrate molecules on the kinetics of the enzymatic reactions was studied. The specificity of α-chymotrypsin in organic media was a combination of its substrate specificity in aqueous media and solvent effects. The kinetics of peptide synthesis can thus be modulated by using suitable solvents and protecting groups.
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| 3. |
- Pettersson, Gösta, et al.
(författare)
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Model studies of the regulation of the Calvin photosynthesis cycle by cytosolic metabolites
- 1990
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Ingår i: Biomedica Biochimica Acta. - 0232-766X. ; 49:8-9, s. 723-732
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Tidskriftsartikel (refereegranskat)abstract
- A kinetic model for photosynthetic carbohydrate formation in the chloroplast of C3 plants is presented which includes consideration of the interaction of the phosphate translocator of the chloroplast envelope with external reactants such as 3-phosphoglycerate, dihydroxyacetone phosphate, and glyceraldehyde 3-phosphate in addition to inorganic (ortho)phosphate. The model is shown to account satisfactorily for experimentally observed effects of such reactants on the rates of carbon dioxide fixation and starch production in isolated chloroplast. The predictions of the model with regard to the regulation of stromal processes of photosynthetic carbohydrate formation by cytosolic concentration variables have been examined. The results indicate that the cytosolic concentrations of metabolites that interact with the phosphate translocator represent important regulatory signals. Increasing levels of exported photosynthetic (phosphoglycerates and triose phosphates) in the cytosol do not suppress the rate of photosynthetic carbon dioxide fixation, but redirects reaction flux such that starch production within the chloroplast is favoured at the expense of a decreased rate of photosynthate export to the cytosol.
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