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- Borrebaeck, Carl, et al.
(författare)
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Protein chips based on recombinant antibody fragments: A highly sensitive approach as detected by mass spectrometry
- 2001
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Ingår i: BioTechniques. - 0736-6205. ; 30:5, s. 1126-1126
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Tidskriftsartikel (refereegranskat)abstract
- With the human genome in a first sequence draft and several other genomes being finished this year, the existing information gap between genomics and proteomics is becoming increasingly evident. The analysis of the proteome is, however, much more complicated because the synthesis and structural requirements of functional proteins are different from the easily handled oligonucleotides for which a first analytical breakthrough already has come in the use of DNA chips. In comparison with the DNA microarrays, the protein arrays, or protein chips, offer the distinct possibility of developing a rapid global analysis of the entire proteome. Thus, the concept of comparing proteomic maps of healthy and diseased cells may allow us to understand cell signaling and metabolic pathways and will form a novel base for pharmaceutical companies to develop future therapeutics much more rapidly. This report demonstrates the possibilities of designing protein chips based on specially constructed, small recombinant antibody fragments using nanostructure surfaces with biocompatible characteristics, resulting in sensitive detection in the 600-amol range. The assay readout allows the determination of single or multiple antigen-antibody interactions. Mass identity of the antigens, currently with a resolution of 8000, enables the detection of structural modifications of single proteins.
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- Buyck, P. J., et al.
(författare)
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Diagnostic accuracy of noncontrast CT imaging markers in cerebral venous thrombosis
- 2019
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Ingår i: Neurology. - : Ovid Technologies (Wolters Kluwer Health). - 0028-3878 .- 1526-632X. ; 92:8
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Tidskriftsartikel (refereegranskat)abstract
- Objective To assess the added diagnostic value of semiquantitative imaging markers on noncontrast CT scans in cerebral venous thrombosis (CVT). Methods In a retrospective, multicenter, blinded, case-control study of patients with recent onset (<2 weeks) CVT, 3 readers assessed (1) the accuracy of the visual impression of CVT based on a combination of direct and indirect signs, (2) the accuracy of attenuation values of the venous sinuses in Hounsfield units (with adjustment for hematocrit levels), and (3) the accuracy of attenuation ratios of affected vs unaffected sinuses in comparison with reference standard MRI or CT angiography. Controls were age-matched patients with (sub)acute neurologic presentations. Results We enrolled 285 patients with CVT and 303 controls from 10 international centers. Sensitivity of visual impression of thrombosis ranged from 41% to 73% and specificity ranged from 97% to 100%. Attenuation measurement had an area under the curve (AUC) of 0.78 (95% confidence interval [CI] 0.74-0.81). After adjustment for hematocrit, the AUC remained 0.78 (95% CI 0.74-0.81). The analysis of attenuation ratios of affected vs unaffected sinuses had AUC of 0.83 (95% CI 0.8-0.86). Adding this imaging marker significantly improved discrimination, but sensitivity when tolerating a false-positive rate of 20% was not higher than 76% (95% CI 0.70-0.81). Conclusion Semiquantitative analysis of attenuation values for diagnosis of CVT increased sensitivity but still failed to identify 1 out of 4 CVT. Classification of evidence This study provides Class II evidence that visual analysis of plain CT with or without attenuation measurements has high specificity but only moderate sensitivity for CVT.
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- Farm, Ante
(författare)
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Om grundutbildningen i nationalekonomi
- 2018
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Ingår i: Ekonomisk Debatt. - 0345-2646. ; 46:6, s. 46-56
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Hur kan studenter få hjälp med att förankra nationalekonomins begrepp och modeller i verkligheten? Förslagsvis genom en introduktion som motsvarar läkarutbildningens inledande kurs i anatomi. Jag föreslår närmare bestämt att grundutbildningen i nationalekonomi inleds med en faktakurs, dvs en kurs som förmedlar grundläggande fakta om en marknadsekonomis institutioner och funktionssätt. Denna artikel ger några exempel på vad en sådan kurs kan innehålla, nämligen grundläggande fakta om marknadsformer och prisbildning, särskilt prisbildningen på marknader för konsumtionsvaror, samt grundläggande fakta om pengar, banker och räntor. Distinktionen mellan fakta, approximationer och teorier diskuteras särskilt.
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- Karlsson, Charlie, et al.
(författare)
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Media clusters and metropolitan knowledge economy
- 2015
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Ingår i: Handbook on the Economics of the Media. - : Edward Elgar Publishing Ltd.. - 9780857938893 - 9780857938886 ; , s. 80-106
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- JOBNAME: Picard PAGE: 1 SESS: 5 OUTPUT: Fri Jan 23 14:35:50 2015 4. Media clusters and metropolitan knowledge economy Charlie Karlsson and Philippe Rouchy Since the mid-1990s, the interrelationship between ICT, the location of media consortia and. ..
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- Kjeld, Thomas, et al.
(författare)
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Cardiac hypoxic resistance and decreasing lactate during maximum apnea in elite breath hold divers
- 2021
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Breath-hold divers (BHD) enduring apnea for more than 4 min are characterized by resistance to release of reactive oxygen species, reduced sensitivity to hypoxia, and low mitochondrial oxygen consumption in their skeletal muscles similar to northern elephant seals. The muscles and myocardium of harbor seals also exhibit metabolic adaptations including increased cardiac lactate-dehydrogenase-activity, exceeding their hypoxic limit. We hypothesized that the myocardium of BHD possesses similar adaptive mechanisms. During maximum apnea 15O-H2O-PET/CT (n = 6) revealed no myocardial perfusion deficits but increased myocardial blood flow (MBF). Cardiac MRI determined blood oxygen level dependence oxygenation (n = 8) after 4 min of apnea was unaltered compared to rest, whereas cine-MRI demonstrated increased left ventricular wall thickness (LVWT). Arterial blood gases were collected after warm-up and maximum apnea in a pool. At the end of the maximum pool apnea (5 min), arterial saturation decreased to 52%, and lactate decreased 20%. Our findings contrast with previous MR studies of BHD, that reported elevated cardiac troponins and decreased myocardial perfusion after 4 min of apnea. In conclusion, we demonstrated for the first time with 15O-H2O-PET/CT and MRI in elite BHD during maximum apnea, that MBF and LVWT increases while lactate decreases, indicating anaerobic/fat-based cardiac-metabolism similar to diving mammals.
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