| 1. |
- Phan, Nhu TN, 1981, et al.
(author)
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Imaging Mass Spectrometry for Single-Cell Analysis
- 2014
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In: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. - : Elsevier. - 9780124095472
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Book chapter (peer-reviewed)abstract
- Single-cell imaging is a very important area in biological, medical, and pharmaceutical research, yet it is one of the most challenging fields for mass spectrometry. Imaging mass spectrometry (IMS), which is a powerful label-free analytical technique, has proved its capability to chemically visualize single cells with high sensitivity, chemical selectivity, and in certain cases subcellular spatial resolution. We present here an overview of the capabilities and current progress of IMS, particularly secondary ion mass spectrometry, matrix-assisted laser desorption ionization, and desorption electrospray ionization for single-cell imaging. The principles and technical developments of each technique are introduced. Critical aspects regarding single-cell imaging are addressed, especially sensitivity, spatial resolution, and sample handling. Current achievements in this field of application are also presented for both 2-D imaging and 3-D imaging. Furthermore, we address the potential contribution of IMS single-cell ‘omics’ and discuss its future development.
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| 2. |
- Willander, M., et al.
(author)
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Potentiometric Biosensors Based on Metal Oxide Nanostructures
- 2017
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In: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. - : Elsevier. - 9780124095472
-
Book chapter (peer-reviewed)abstract
- Numerous potentiometric biosensors are fabricated via biocatalytic and bioaffinity-based biosensing mechanisms. Only few of them are useful and applicable to the biomedical application and analysis. The most of those sensing schemes are mainly related to the protein metabolism especially urea and creatinine. The emergence of nanoscience and nanotechnology in the biomedical applications has provided the solid platform for the development of sensitive and selective potentiometric biosensors as new generation analytical devices. Therefore, among the nanomaterials, metal oxides are of prime importance for the potentiometric analytical devices due to generation of strong potential signals and excellent biocompatibility with the proteins such as enzymes, antibodies, DNA, and biological cells. This book chapter is dedicated to the recent advancement in the development of potentiometric biosensors such as urea, uric acid, glucose, and cholesterol due to nanoscience from fundamental to advanced configuration approach of devices.
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