| 1. |
- Akbari, Elham, et al.
(author)
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SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT
- 2021
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In: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 1201-1202
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Conference paper (peer-reviewed)abstract
- Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.
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| 2. |
- Akbari, Elham, et al.
(author)
-
SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION
- 2022
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In: MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419048 ; , s. 306-307
-
Conference paper (peer-reviewed)abstract
- Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.
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