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- Ghosheh, Nidal, et al.
(author)
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Comparative transcriptomics of hepatic differentiation of human pluripotent stem cells and adult human liver tissue
- 2017
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In: Physiological Genomics. - : American Physiological Society. - 1094-8341 .- 1531-2267. ; 49:8, s. 430-446
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Journal article (peer-reviewed)abstract
- Hepatocytes derived from human pluripotent stem cells (hPSC-HEP) have the potential to replace presently used hepatocyte sources applied in liver disease treatment and models of drug discovery and development. Established hepatocyte differentiation protocols are effective and generate hepatocytes, which recapitulate some key features of their in vivo counterparts. However, generating mature hPSC-HEP remains a challenge. In this study, we applied transcriptomics to investigate the progress of in vitro hepatic differentiation of hPSCs at the developmental stages, definitive endoderm, hepatoblasts, early hPSC-HEP, and mature hPSC-HEP, to identify functional targets that enhance efficient hepatocyte differentiation. Using functional annotation, pathway and protein interaction network analyses, we observed the grouping of differentially expressed genes in specific clusters representing typical developmental stages of hepatic differentiation. In addition, we identified hub proteins and modules that were involved in the cell cycle process at early differentiation stages. We also identified hub proteins that differed in expression levels between hPSC-HEP and the liver tissue controls. Moreover, we identified a module of genes that were expressed at higher levels in the liver tissue samples than in the hPSC-HEP. Considering that hub proteins and modules generally are essential and have important roles in the protein-protein interactions, further investigation of these genes and their regulators may contribute to a better understanding of the differentiation process. This may suggest novel target pathways and molecules for improvement of hPSC-HEP functionality, having the potential to finally bring this technology to a wider use.
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| 3. |
- Ghosheh, Nidal, 1975-, et al.
(author)
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Human Pluripotent Stem Cell-Derived Hepatocytes Show Higher Transcriptional Correlation with Adult Liver Tissue than with Fetal Liver Tissue
- 2020
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In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 5:10, s. 4816-4827
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Journal article (peer-reviewed)abstract
- Human pluripotent stem cell-derived hepatocytes (hPSC-HEP) display many properties of mature hepatocytes, including expression of important genes of the drug metabolizing machinery, glycogen storage, and production of multiple serum proteins. To this date, hPSC-HEP do not, however, fully recapitulate the complete functionality of in vivo mature hepatocytes. In this study, we applied versatile bioinformatic algorithms, including functional annotation and pathway enrichment analyses, transcription factor binding-site enrichment, and similarity and correlation analyses, to datasets collected from different stages during hPSC-HEP differentiation and compared these to developmental stages and tissues from fetal and adult human liver. Our results demonstrate a high level of similarity between the in vitro differentiation of hPSC-HEP and in vivo hepatogenesis. Importantly, the transcriptional correlation of hPSC-HEP with adult liver (AL) tissues was higher than with fetal liver (FL) tissues (0.83 and 0.70, respectively). Functional data revealed mature features of hPSC-HEP including cytochrome P450 enzymes activities and albumin secretion. Moreover, hPSC-HEP showed expression of many genes involved in drug absorption, distribution, metabolism, and excretion. Despite the high similarities observed, we identified differences of specific pathways and regulatory players by analyzing the gene expression between hPSC-HEP and AL. These findings will aid future intervention and improvement of in vitro hepatocyte differentiation protocol in order to generate hepatocytes displaying the complete functionality of mature hepatocytes. Finally, on the transcriptional level, our results show stronger correlation and higher similarity of hPSC-HEP to AL than to FL. In addition, potential targets for further functional improvement of hPSC-HEP were also identified.
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- Pramanik, Roshni, et al.
(author)
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Harbour Battery Energy Storage Systems: Hazards and potential mitigation measures
- 2023
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In: Brandposten. - : RISE Research Institutes of Sweden. ; :62, s. 28-29
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Journal article (other academic/artistic)abstract
- Battery Energy Storage Systems (BESS) is a vital part of electrification of the shipping industry. However, there are potential risks that must be considered for BESS installation. The article presents HAZID as an earlystage hazard identification method for installation of harbour BESS. The HAZID identified critical factors such as proximity to the marine environment and safe distances between BESS containers
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- Silfvernagel, Kristin, et al.
(author)
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Individually tailored internet-based cognitive behaviour therapy for older adults with anxiety and depression : a randomised controlled trial
- 2018
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In: Cognitive Behaviour Therapy. - : Taylor & Francis Group. - 1650-6073 .- 1651-2316. ; 47:4, s. 286-300
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Journal article (peer-reviewed)abstract
- Mixed anxiety and depression is common among older adults. The aim of the study was to compare the effects of an eight-week-long tailored internet-supported cognitive behaviour therapy (ICBT) programme and to compare against the provision of weekly general support. A second aim was to investigate if pre-treatment cognitive flexibility and self-reported cognitive problems would predict outcome. We included 66 older adults (aged over 60 years) with mixed anxiety/depression following media recruitment and randomised them into treatment and control groups. We also included a one-year follow-up. As a measure of executive function, we used the Wisconsin Card Sorting Test (perseverative errors) and the Cognitive Failures Questionnaire during the pre-treatment phase. Results showed a moderate between-group effect on the main outcome measure, the Beck Anxiety Inventory (BAI) (d= .50), favouring the treatment group. Nearly half (45.5%) of that group were classified as responders. One person (3%) in the treatment group deteriorated. There were significant correlations between perseverative errors and outcome (on the BAI r = -.45), but not among self-reported cognitive function. We conclude that guided, tailored ICBT may be effective for some older adults and that the role of cognitive function needs to be investigated further.
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- Adielsson, Stina, et al.
(author)
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Resultat från miljöövervakningen av bekämpningsmedel (växtskyddsmedel) : Långtidsöversikt 2002-2008. Årssammanställning 2008
- 2009. - 2009:115
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Reports (other academic/artistic)abstract
- Rapporten presenterar resultaten från övervakningen av växtskyddsmedel i ytvatten, grundvatten, sediment och regnvatten i Sverige under åren 2002-2008. Undersökningen genomförs inom ramen för den nationella miljöövervakningen på uppdrag av Naturvårdsverket (Jordbruksmark och Luft). Tidigare resultat har sammanställts i enskilda årsrapporter. Detta är första gången som resultaten har sammanställts för en längre tidsperiod (7 år), vilket ger ett underlag för att spegla utvecklingen över tiden.Övervakningen av ytvatten omfattas av provtagningar i fyra typområden (Västergötland, Figur 2; Östergötland, Figur 5; Halland, Figur 8; Skåne, Figur 11) och två åar i Skåne (Skivarpsån och Vege å). Ytvattenprovtagningen står för den största andelen av alla enskilda analyser under perioden (ca 60 000). Provtagningarna i typområdena sker tidsintegrerat med hjälp av automatiska provtagare och i åarna tas manuella momentanprov. I typområdena har även provtagning av grundvatten och sediment genomförts. Regnvatten undersöks vid en lokal på Söderåsen i Skåne.Antalet substanser som har analyserats varierar mellan provtyperna och i viss mån även mellan åren. Detta som en följd av att vissa substanser försvinner från marknaden och nya tillkommer. Även analysgränsen har förändrats under åren, främst för några substanser där den sänkts för att bättre motsvara de riktvärden som introducerats under tidsperioden.Inom varje typområde samlas det varje år in omfattande informaion om grödval och vilka växtskyddsmedel som används. Även information om nederbörd och avrinning samlas in kontinuerligt. Denna information utgör ett värdefullt underlag vid tolkningen av resultaten.
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| 8. |
- Andersson, Agneta, et al.
(author)
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Aquatic ecosystems at risk for occurrence of pathogenic bacteria
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Other publication (other academic/artistic)abstract
- Pathogenic bacteria occur naturally in aquatic systems. Co-existence of bacteria and protozoa has led to development of predation resistance strategies, which is suggested to serve as a driver for evolution of pathogenic bacteria. However, the ecological mechanisms for selection for different types of predation resistant and pathogenic bacteria are poorly known. To disentangle effects from nutrient availability and protozoan predation pressure on the occurrence of varying predation resistant bacterial genera, an enrichment-dilution experiment was performed where an aquatic bacterial community was exposed to protozoa. Operational taxonomical units, specific for three predation resistant bacterial genera were identified; Pseudomonas, Rickettsia and Mycobacterium. These genera are also known to harbor species that are potentially pathogenic to mammals. Rickettsia and Mycobacterium were promoted where protozoa were abundant and the predation pressure high, while Pseudomonas dominated the bacterial community at the highest nutrient level where the predation pressure on bacteria were low. Our study thus indicates that waters of all nutrient states can harbor pathogenic bacteria, but that bacteria with different ecological strategies occur depending on nutrient level and perturbation. The generative model approach presented here provide a possibility to integrate environmental data in prediction models of pathogens in complex environments.
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| 10. |
- Andersson, Elisabeth, et al.
(author)
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Aktuellt kunskapsläge vid kateterspolning : systematisk litteraturgranskning
- 2011
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In: Vård i Norden. - : Sykepleiernes Samarbeid i Norden (SSN). - 0107-4083 .- 1890-4238. ; 31:4, s. 27-31
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Journal article (peer-reviewed)abstract
- Aim: Reviewing the scientific literature with regards to urinary catheter washouts in order to prevent occlusion. Background: Patients with long-term indwelling urinary catheters often suffers from catheter blockage. Encrustations and the forming of biofilm on the catheter surface are among the most common causes of occlusion. It is unknown whether urinary catheter washout is the treatment of choice to prevent occlusion. Methods: A systematic review of the scientific literature regarding urinary catheter washouts. The study was conducted by two independent reviewers. The search used MeSH and freetext terms, in The Cochrane Database of Systematic Reviews, Academic Search Elite, CINAHL, MedLine and SweMed+, and an additional mapping of clinical guidelines in order to update praxis. Findings: The study shows that there is insufficient scientific evidence as to whether urinary catheter washouts prevent catheter blockage. Conclusion: There is insufficient evidence that urinary catheter washouts are beneficial. Individual care planning and evaluation of long-term indwelling catheter care is important in minimizing the risk of harmful side effects.
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