| 1. |
- Antiochia, Riccarda, et al.
(författare)
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A new osmium-polymer modified screen-printed graphene electrode for fructose detection
- 2014
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Ingår i: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 195, s. 287-293
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes the development and performance of the first fructose biosensor based on a commercial screen-printed graphene electrode (SPGE). The electrode was modified with an osmium-polymer, which allowed the efficient wiring of the enzyme fructose dehydrogenase (FDH). The immobilization of both osmium-polymer and FDH was realized in an easy way. Aliquots of 10 mu L, Os-polymer and 10 mu L, FDH were thoroughly mixed with poly(ethylene glycol) (400) diglycidyl ether (PEDGE) and deposited on the electrode surface and left there to dry overnight. The biosensor exhibits a detection limit of 0.8 mu M, a linear range between 0.1 and 8 mM, high sensitivity to fructose (2.15 mu A cm(-2)/mM), good reproducibility (RSD = 1.9%), fast response time (3 s) and a stability of 2 months when stored in the freezer. The proposed fructose biosensor was tested in real food samples and validated with a commercial spectrophotometric enzymatic kit. No significant interference was observed with the proposed biosensor. Published by Elsevier B.V.
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| 2. |
- Antiochia, Riccarda, et al.
(författare)
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Composite Material Based on Macroporous Polyaniline and Osmium Redox Complex for Biosensor Development
- 2014
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Ingår i: Electroanalysis. - : Wiley. - 1040-0397. ; 26:7, s. 1623-1630
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Tidskriftsartikel (refereegranskat)abstract
- Here the feasibility of layers based on the conducting polymer polyaniline (PANI) as component of glucose biosensors using glucose oxidase (GOx) as enzyme and [Os(bpy)(2)(4-aminomethylpyridine)Cl]PF6 (OsCmplx) as electrochemical mediator, is evaluated. Particularly, PANI was employed to obtain a nanostructured macroporous material (m-PANI) around polystyrene nanoparticles taken as template and the mediator was co-immobilized during the polymerizing procedure. The GOx biosensor based on OsCmplx modified m-PANI provides a linear response to glucose concentration in the range 5 up to 65 mM with a sensitivity of 3.54 mu A/mM/cm(2) (on a projected geometric area=0.07 cm(2)), an LOD of 0.8 mM and a good precision (%RSD <= 7, n=5); the biosensor is stable showing a decrease of 10% to the value of the sensitivity after 15 days of use and of about 50% after 40 days.
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| 3. |
- Antiochia, Riccarda, et al.
(författare)
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Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages
- 2007
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Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 22:11, s. 2611-2617
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.
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| 4. |
- Antiochia, Riccarda, et al.
(författare)
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Graphene and 2D-Like Nanomaterials : Different Biofunctionalization Pathways for Electrochemical Biosensor Development
- 2017
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Ingår i: Graphene Bioelectronics. - 9780128133507 - 9780128133491 ; , s. 1-35
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Bokkapitel (refereegranskat)abstract
- In the last decades, nanotechnology has played a key role in the electrochemical biosensor development based on the mediated and direct electrochemical communication between the biorecognition elements and the electrode surface. In particular, graphene and 2D-like nanomaterials (e.g., boron nitride nanosheets, graphitic carbon nitride (g-C3N4) nanosheets, and various transition metal dichalcogenides) have attracted an increasing interest due to their peculiar properties such as high specific surface area and the ease of biofunctionalization. Moreover, in the last few years, the successful integration of graphene and 2D-like nanomaterials with other nanomaterials such as metal nanoparticles, metal oxides, or quantum dots has dramatically increased the opportunities to develop novel electrochemical biosensors with highly enhanced performances, mainly due to the synergistic effects.In this chapter, we would like to give the state of art of graphene and 2D-like nanomaterials employment for electrochemical biosensors development, by critically discussing the advantages and drawbacks. Successively, the discussion should be separately addressed to three different cases: (1) redox enzyme immobilization, (2) antibody immobilization, and (3) DNA/aptamer immobilization. Finally, we should critically define for which cases graphene and 2D-like nanomaterials are the most suitable electrochemical platform, within some conclusion remarks and future perspectives of both kinds of nanomaterials.
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| 5. |
- Antiochia, Riccarda, et al.
(författare)
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Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor
- 2013
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Ingår i: Food Chemistry. - : Elsevier BV. - 1873-7072 .- 0308-8146. ; 140:4, s. 742-747
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved.
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| 6. |
- Bollella, Paolo, et al.
(författare)
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A Glucose/Oxygen Enzymatic Fuel Cell based on Gold Nanoparticles modified Graphene Screen-Printed Electrode. Proof-of-Concept in Human Saliva
- 2018
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Ingår i: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 256, s. 921-930
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a new direct electron transfer based-miniaturized glucose/oxygen enzymatic fuel cell (EFC) whose operating ability has been tested in real saliva samples. The bioanode and biocathode are a graphene working electrode and a graphite counter electrode localized on the same screen printed electrode (SPE) modified with poly(vinyl alcohol) N-methyl-4(4'-formylstyryl)pyridinium methosulfate acetal (PVA-SbQ)/cellobiose dehydrogenase from Corynascus Thermophilus (CtCDH) C291Y/AuNPs and with Trametes Hirsuta laccase (ThLac)/AuNPs, respectively.In order to optimize the bioanode, several CDH immobilization procedures were adopted, such as drop-casting, use of Nafion membrane or PVA-SbQ photopolymer. The photopolymer showed the best performance in terms of stability and reliability. As biocathode a partially optimized laccase electrode was employed with the variant that the used nanomaterials allowed to reduce the overpotential of O2/H2O redox reaction catalyzed by Trametes Hirsuta Laccase (ThLac), drop-casted onto the gold nanoparticles (AuNPs) modified SPE.The performances of bioanode and biocathode were tested separately, initially immobilizing the two enzymes onto separated graphene SPEs. An efficient direct electron transfer was achieved for both elements, obtaining an apparent heterogeneous electron transfer rate constant (ks ) of 0.99±0.05s-1 for CtCDH C291Y and 5.60±0.05s-1 for ThLac. Both electrodes were then assembled in a two compartment EFC obtaining a maximal power output of 5.16±0.15μWcm-2 at a cell voltage of 0.58V and an open circuit voltage (OCV) of 0.74V. Successively, the bioanode and biocathode were assembled in a non-compartmentalized EFC and a remarkable 50% decrease of the maximum power output at the value of 2.15±0.12μWcm-2 at cell voltage of 0.48V and an OCV of 0.62V at pH 6.5 was registered. In order to reduce the cell dimensions in view of its possible integration in biomedical devices, the bioanode and biocaythode were realized by immobilization of both enzymes onto the same SPE. The so miniaturized EFC delivered a maximal power output of 1.57±0.07μWcm2 and 1.10±0.12μWcm-2 with an OCV of 0.58V and 0.41V in a 100μM glucose solution and in human saliva, respectively.
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| 7. |
- Bollella, Paolo, et al.
(författare)
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A third generation glucose biosensor based on cellobiose dehydrogenase immobilized on a glassy carbon electrode decorated with electrodeposited gold nanoparticles : Characterization and application in human saliva
- 2017
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 17:8
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Tidskriftsartikel (refereegranskat)abstract
- Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19.4 ± 0.6 s−1, with an enhanced theoretical and real enzyme surface coverage (Γtheor and Γreal) of 5287 ± 152 pmol cm−2 and 27 ± 2 pmol cm−2, respectively. The modified electrode was successively used as glucose biosensor exhibiting a detection limit of 6.2 μM, an extended linear range from 0.02 to 30 mM, a sensitivity of 3.1 ± 0.1 μA mM−1 cm−2 (R2 = 0.995), excellent stability and good selectivity. These performances compared favourably with other glucose biosensors reported in the literature. Finally, the biosensor was tested to quantify the glucose content in human saliva samples with successful results in terms of both recovery and correlation with glucose blood levels, allowing further considerations on the development of non-invasive glucose monitoring devices.
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| 8. |
- Bollella, Paolo, et al.
(författare)
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Beyond graphene : Electrochemical sensors and biosensors for biomarkers detection
- 2017
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Ingår i: Biosensors and Bioelectronics. - : Elsevier BV. - 0956-5663. ; 89, s. 152-166
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Tidskriftsartikel (refereegranskat)abstract
- Graphene's success has stimulated great interest and research in the synthesis and characterization of graphene-like 2D materials, single and few-atom-thick layers of van der Waals materials, which show fascinating and technologically useful properties. This review presents an overview of recent electrochemical sensors and biosensors based on graphene and on graphene-like 2D materials for biomarkers detection. Initially, we will outline different electrochemical sensors and biosensors based on chemically derived graphene, including graphene oxide and reduced graphene oxide, properly functionalized for improved performances and we will discuss the various strategies to prepare graphene modified electrodes. Successively, we present electrochemical sensors and biosensors based on graphene-like 2D materials, such as boron nitride (BN), graphite-carbon nitride (g-C3N4), transition metal dichalcogenides (TMDs), transition metal oxides and graphane, outlining how the new modified 2D nanomaterials will improve the electrochemical performances. Finally, we will compare the results obtained with different sensors and biosensors for the detection of important biomarkers such as glucose, hydrogen peroxide and cancer biomarkers and highlight the advantages and disadvantages of the use of graphene and graphene-like 2D materials in different sensing platforms.
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| 9. |
- Bollella, Paolo, et al.
(författare)
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Direct electron transfer of dehydrogenases for development of 3rd generation biosensors and enzymatic fuel cells
- 2018
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Ingår i: Sensors (Switzerland). - : MDPI AG. - 1424-8220. ; 18:5
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Forskningsöversikt (refereegranskat)abstract
- Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process. In this review we briefly describe the electron transfer mechanism of dehydrogenase enzymes and some of the characteristics required for bioelectrocatalysis reactions via a direct electron transfer mechanism. Special attention is given to cellobiose dehydrogenase and fructose dehydrogenase, which showed efficient direct electron transfer reactions. An overview of the most recent biosensors and biofuel cells based on the two dehydrogenases will be presented. The various strategies to prepare modified electrodes in order to improve the electron transfer properties of the device will be carefully investigated and all analytical parameters will be presented, discussed and compared.
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| 10. |
- Bollella, Paolo, et al.
(författare)
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Enhanced Direct Electron Transfer of Fructose Dehydrogenase Rationally Immobilized on a 2-Aminoanthracene Diazonium Cation Grafted Single-Walled Carbon Nanotube Based Electrode
- 2018
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Ingår i: ACS Catalysis. - : American Chemical Society (ACS). - 2155-5435. ; , s. 10279-10289
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy-carbon electrode (GCE) upon which anthracene-modified single-walled carbon nanotubes were deposited. The SWCNTs were activated in situ with a diazonium salt synthesized through the reaction of 2-aminoanthracene with NaNO2 in acidic media (0.5 M HCl) for 5 min at 0 °C. After the in situ reaction, the 2-aminoanthracene diazonium salt was electrodeposited by running cyclic voltammograms from +1000 to -1000 mV. The anthracene-SWCNT-modified GCE was further incubated in an FDH solution, allowing enzyme adsorption. Cyclic voltammograms of the FDH-modified electrode revealed two couples of redox waves possibly ascribed to the heme c1 and heme c3 of the cytochrome domain. In the presence of 10 mM fructose two catalytic waves could clearly be seen and were correlated with two heme cs (heme c1 and c2), with a maximum current density of 485 ± 21 μA cm-2 at 0.4 V at a sweep rate of 10 mV s-1. In contrast, for the plain SWCNT-modified GCE only one catalytic wave and one couple of redox waves were observed. Adsorbing FDH directly onto a GCE showed no non-turnover electrochemistry of FDH, and in the presence of fructose only a slight catalytic effect could be seen. These differences can be explained by considering the hydrophobic pocket close to heme c1, heme c2, and heme c3 of the cytochrome domain at which the anthracenyl aromatic structure could interact through π-π interactions with the aromatic side chains of the amino acids present in the hydrophobic pocket of FDH.
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