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- Asplund, Annika, 1979-, et al.
(author)
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One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells
- 2016
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In: Stem Cell Reviews and Reports. - : Springer Science and Business Media LLC. - 1550-8943 .- 1558-6804. ; 12:1, s. 90-104
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Journal article (peer-reviewed)abstract
- Human hepatocytes display substantial functional inter-individual variation regarding drug metabolizing functions. In order to investigate if this diversity is mirrored in hepatocytes derived from different human pluripotent stem cell (hPSC) lines, we evaluated 25 hPSC lines originating from 24 different donors for hepatic differentiation and functionality. Homogenous hepatocyte cultures could be derived from all hPSC lines using one standardized differentiation procedure. To the best of our knowledge this is the first report of a standardized hepatic differentiation procedure that is generally applicable across a large panel of hPSC lines without any adaptations to individual lines. Importantly, with regard to functional aspects, such as Cytochrome P450 activities, we observed that hepatocytes derived from different hPSC lines displayed inter-individual variation characteristic for primary hepatocytes obtained from different donors, while these activities were highly reproducible between repeated experiments using the same line. Taken together, these data demonstrate the emerging possibility to compile panels of hPSC-derived hepatocytes of particular phenotypes/genotypes relevant for drug metabolism and toxicity studies. Moreover, these findings are of significance for applications within the regenerative medicine field, since our stringent differentiation procedure allows the derivation of homogenous hepatocyte cultures from multiple donors which is a prerequisite for the realization of future personalized stem cell based therapies.
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| 2. |
- Fransson, Åsa, 1971, et al.
(author)
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A swedish grouting design concept: Hydraulic testing and selection of grout
- 2012
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In: Geotechnical Special Publication. - Reston, VA : American Society of Civil Engineers. - 0895-0563. - 9780784412350 ; :228 GSP, s. 1691-1700
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Conference paper (peer-reviewed)abstract
- Some grouting boreholes take no grout and some boreholes take too much, two extremes related to grouting technique, grout properties and the properties of fractures intersecting the boreholes. Successful sealing of rock and soil demands an adequate description of the system to be grouted as a basis for grouting design and selection of grouting material. The basis for this Swedish concept of grouting design is the individual fractures and the hydraulic apertures, b, of these fractures. The hydraulic aperture is an important parameter to describe the grouting behavior and is used to determine if the grout can enter the fractures, the penetrability. The hydraulic aperture also determines the penetration length in addition to grout parameters e.g. yield stress, τ0, and viscosity, μg as well as grouting pressure and time. Knowing these parameters, a proper grouting technique can be adapted. Important input for both design and performance are simple and practical tests of rock and grout and the intention of this paper is to present a testing procedure and give examples from laboratory and field experiences that the approach actually works. © 2012 American Society of Civil Engineers.
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