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- Turkez, H., et al.
(author)
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In vitro transcriptome response to propolis in differentiated SH-SY5Y neurons
- 2021
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In: Journal of food biochemistry. - : Wiley. - 0145-8884 .- 1745-4514. ; 45:12
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Journal article (peer-reviewed)abstract
- Propolis is the extract of a resinous compound that protects plants from both cold and microorganism attack and has gained a strong and sticky property because it is transformed after being collected by honey bees. Up to date, many studies have shown that propolis exhibited various beneficial biological activities, such as antifungal, antibacterial, antiviral, antioxidant, antimutagenic, and antitumor effects. Recent reports propounded the in vitro and in vivo neuroprotective effect of propolis; however, the exact molecular genetic mechanisms are still unclear. Therefore, we aimed to investigate the toxicogenomic and beneficial properties, including cytotoxic, antioxidant, apoptotic/necrotic as well as genotoxic effects of propolis (1.56–200 µg/ml) on differentiated SH-SY5Y neuronal cells. Additionally, microarray analysis was conducted on cell cultures following propolis application to explore gene differentiation. Differentially expressed genes were further analyzed using string software to characterize protein–protein interactions between gene pathways. Our results revealed that propolis applications could not have a prominent effect on cell viability even at concentrations up to 200 µg/ml. The highest propolis concentration induced apoptotic rather than necrotic cell death. The alterations in gene expression profiles, including CYP26A1, DHRS2, DHRS3, DYNC1I1, IGF2, ITGA4, SVIL, TGFβ1, and TGM2 could participate in the neuroprotective effects of propolis. In conclusion, propolis supplementation exerted remarkable advantageous; thus, it may offer great potential as a natural component in the prevention and treatment of neurodegenerative disorders. Whole-genome gene expression pattern following propolis application was investigated for the first time in neuronal cell culture to fill a gap in the literature about propolis toxicogenomics. Practical applications: Propolis is a very rich product in terms of benefits. In addition to its antibacterial, antiviral, antifungal, and anti-inflammatory content, it is known to have preventive and therapeutic properties for many different ailments. On the other hand, molecular mechanisms of propolis on gene expression differentiations haven't been investigated until now. Moreover, gene expression pattern is vital for all living organisms to maintain homeostasis. Thus, we conduct an experiment series for analyzing gene expression differentiation effects on neuronal cells to understand beneficial properties of propolis. Hence, it could be possible to comment on the use of propolis as a nutritional factor and beneficial diet.
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| 3. |
- Çaglar, E, et al.
(author)
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Effect of yoghurt with Bifidobacterium DN-173010 on salivary mutans streptococci and lactobacilli
- 2005
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In: Acta odontologica Scandinavica. ; 63:6, s. 317-320
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Journal article (peer-reviewed)abstract
- Abstract. Probiotic bacteria are thought to reduce the risk of disease. Previous studies have suggested that lactobacilli-derived probiotics in dairy products may affect the oral ecology but the effect of bifidobacteria has not previously been reported. The aim of the present study was to examine whether or not a short-term consumption of yogurt containing bifidobacteria would affect the salivary levels of mutans streptococci and lactobacilli in young adults. A double blind, randomized crossover study was performed and 21 healthy subjects were followed over four periods. During period 2 and 4 (two weeks each), they ingested 200 g yogurt containing Bifidobacterium DN-173 010 once daily or a control yogurt without viable bacteria. Periods 1 and 3 were run-in and washout periods, respectively. Salivary mutans streptococci and lactobacilli were enumerated with chair-side kits. A statistically significant reduction (p<0.05) of salivary mutans streptococci was recorded after the probiotic yogurt consumption, which was in contrast to the controls. A similar trend was seen for lactobacilli but this decrease failed to reach statistical significance. In conclusion, probiotic bifidobacteria in yogurt may reduce the levels of selected caries-associated microorganisms in saliva.
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| 4. |
- Çaglar, E, et al.
(author)
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Probiotics and oral health.
- 2006
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In: Prophylaxe impuls. ; :10, s. 62-68
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Journal article (peer-reviewed)abstract
- Probiotics are living microorganisms that beneficially affect the host by improving its intestinal microbial balance. While the role of probiotics in preventive and therapeutic medicine is firmly established, only limited research is available on its effect on oral health. Recent findings have however suggested that strains of Lactobacillus and Bifidobacterium may inhibit mutans streptococci and candida species in the oral cavity. The present overview focuses on vehicles, installation in the mouth and possible benefits on oral ecology. Although an increasing number of studies suggest a role of lactobacilli-derived probiotics on caries pathogens, its efficacy as a preventive measure is still to be determined in randomized controlled trials.
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| 5. |
- Çaglar, E, et al.
(author)
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Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws and tablets.
- 2006
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In: Acta odontologica Scandinavica. ; :64, s. 314-318
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Journal article (peer-reviewed)abstract
- OBJECTIVE: Previous studies have suggested that lactobacilli-derived probiotics in dairy products may affect oral ecology, but the impact of different vehicles of ingestion has received little attention. The aim of the present study was to investigate the effect of the probiotic bacterium Lactobacillus reuteri ATCC 55730 on the levels of salivary mutans streptococci and lactobacilli in young adults when ingested by two different delivery systems. MATERIAL AND METHODS: The material comprised 120 healthy young adults (21-24 years) and a placebo-controlled study design with parallel arms was utilized. The subjects were randomly assigned to four equally sized groups: group A drank 200 ml of water through a prepared straw containing L. reuteri ATCC 55730 once daily for 3 weeks, while group B took 200 ml water through a placebo straw during the same period. Group C was given one tablet containing L. reuteri ATCC 55730 once daily for 3 weeks, while group D received placebo tablets without bacteria. Salivary mutans streptococci and lactobacilli were enumerated with chair-side kits at baseline and 1 day after the final ingestion. RESULTS: A statistically significant reduction of the mutans streptococci levels was recorded after ingestion of the probiotic bacteria via the straw (p<0.05) and the tablets (p<0.01), which was in contrast to the placebo controls. A similar but non-significant trend was seen for lactobacilli. CONCLUSIONS: A short-term daily ingestion of lactobacilli-derived probiotics delivered by prepared straws or lozenges reduced the levels of salivary mutans streptococci in young adults.
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| 6. |
- Caglar, K, et al.
(author)
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Serum fetuin-a concentration and endothelial dysfunction in chronic kidney disease
- 2008
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In: Nephron. Clinical practice. - : S. Karger AG. - 1660-2110. ; 108:3, s. C233-C240
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Journal article (peer-reviewed)abstract
- <i>Background:</i> Defective endothelial function, an initial step in the development of atherosclerotic plaque, is prevalent in moderate to advanced chronic kidney disease (CKD). In this study, the investigators hypothesized that fetuin-A, a calcification inhibitor, is a novel risk factor for the development of endothelial dysfunction in patients. <i>Methods:</i> 198 nondiabetic patients with a mean age of 44.0 ± 12.4 years and with different stages of CKD were studied. In addition to a detailed metabolic panel, flow-mediated dilatation assessed by high-resolution brachial ultrasonography was performed to determine endothelial dysfunction. Carotid intima-media thickness was also estimated by ultrasonography. Serum fetuin-A concentrations were determined by using a human ELISA method. <i>Results:</i> Endothelial dysfunction was observed in all stages (1–5) of CKD and worsened in parallel to the reduction in estimated glomerular filtration rate. Serum fetuin-A concentrations were also found to be decreased in all but stage 1 CKD. On multiple regression analysis, endothelial dysfunction was independently associated with fetuin-A (β = 0.745, p < 0.001) and intact parathyroid hormone concentrations (β = –0.216, p < 0.001). <i>Conclusion:</i> These data in a selected cohort of CKD patients indicate that fetuin-A may be one of the contributing factors for the development of endothelial dysfunction in CKD patients.
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