| 1. |
- Bandaru, Sunith, et al.
(author)
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Development, analysis and applications of a quantitative methodology for assessing customer satisfaction using evolutionary optimization
- 2015
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In: Applied Soft Computing. - : Elsevier. - 1568-4946 .- 1872-9681. ; 30, s. 265-278
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Journal article (peer-reviewed)abstract
- Consumer-oriented companies are getting increasingly more sensitive about customer's perception of their products, not only to get a feedback on their popularity, but also to improve the quality and service through a better understanding of design issues for further development. However, a consumer's perception is often qualitative and is achieved through third party surveys or the company's recording of after-sale feedback through explicit surveys or warranty based commitments. In this paper, we consider an automobile company's warranty records for different vehicle models and suggest a data mining procedure to assign a customer satisfaction index (CSI) to each vehicle model based on the perceived notion of the level of satisfaction of customers. Based on the developed CSI function, customers are then divided into satisfied and dissatisfied customer groups. The warranty data are then clustered separately for each group and analyzed to find possible causes (field failures) and their relative effects on customer's satisfaction (or dissatisfaction) for a vehicle model. Finally, speculative introspection has been made to identify the amount of improvement in CSI that can be achieved by the reduction of some critical field failures through better design practices. Thus, this paper shows how warranty data from customers can be utilized to have a better perception of ranking of a product compared to its competitors in the market and also to identify possible causes for making some customers dissatisfied and eventually to help percolate these issues at the design level. This closes the design cycle loop in which after a design is converted into a product, its perceived level of satisfaction by customers can also provide valuable information to help make the design better in an iterative manner. The proposed methodology is generic and novel, and can be applied to other consumer products as well.
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| 2. |
- Gaur, Rahul, et al.
(author)
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Diet-dependent depletion of queuosine in tRNAs in Caenorhabditis elegans does not lead to a developmental block.
- 2007
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In: J Biosci. - 0250-5991. ; 32:4, s. 747-54
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Journal article (peer-reviewed)abstract
- Queuosine (Q), a hypermodified nucleoside,occurs at the wobble position of transfer RNAs (tRNAs)with GUN anticodons. In eubacteria, absence of Q affects messenger RNA (mRNA) translation and reduces the virulence of certain pathogenic strains. In animal cells,changes in the abundance of Q have been shown to correlate with diverse phenomena including stress tolerance, cell proliferation and tumour growth but the function of Q in animals is poorly understood. Animals are thought to obtain Q (or its analogues) as a micronutrient from dietary sources such as gut micro flora. However,the difficulty of maintaining animals under bacteria-free conditions on Q-deficient diets has severely hampered the study of Q metabolism and function in animals. In this study,we show that as in higher animals, tRNAs in the nematode Caenorhabditis elegans are modified by Q and its sugar derivatives. When the worms were fed on Q-deficient Escherichia coli, Q modification was absent from the worm tRNAs suggesting that C.elegans lacks a de novo pathway of Q biosynthesis. The inherent advantages of C.elegans as a model organism, and the simplicity of conferring a Q-deficient phenotype on it make it an ideal system to investigate the function of Q modification in tRNA.
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| 3. |
- Lidström, Tommy, et al.
(author)
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Extracellular galectin 4 drives immune evasion and promotes T-cell apoptosis in pancreatic cancer
- 2023
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In: Cancer immunology research. - : American Association for Cancer Research. - 2326-6066 .- 2326-6074. ; 11:1, s. 72-92
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Journal article (peer-reviewed)abstract
- Pancreatic ductal adenocarcinoma (PDAC) is characterized by rich deposits of extracellular matrix (ECM), affecting the pathophysiology of the disease. Here, we identified galectin 4 (gal 4) as a cancer cell produced protein deposited into the ECM of PDAC tumors and detected high circulating levels of gal 4 in PDAC patients. In orthotopic transplantation experiments we observed increased infiltration of T-cells and prolonged survival in immunocompetent mice transplanted with cancer cells with reduced expression of gal 4. Increased survival was not observed in immunodeficient RAG1-/- mice, demonstrating that the effect was mediated by the adaptive immune system. Furthermore, by performing single-cell RNA-sequencing we found that the myeloid compartment and cancer-associated fibroblast (CAF) subtypes were altered in the transplanted tumors. Reduced gal 4 expression was associated with higher proportion of myofibroblastic CAFs and reduced numbers of inflammatory CAFs. We also found higher proportions of M1 macrophages, T-cells and antigen presenting dendritic cells in tumors with reduced gal 4 expression. Using a co-culture system, we observed that extracellular gal 4 induced apoptosis in T-cells by binding N-glycosylation residues on CD3 epsilon/delta. Hence, we show that gal 4 is involved in immune evasion and identify gal 4 as a promising drug target for overcoming immunosuppression in PDAC.
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| 4. |
- Natarajan, Balasubramanian, 1981-, et al.
(author)
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Depletion of the ER chaperone ENPL-1 sensitizes C. elegans to the anticancer drug cisplatin
- 2013
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In: Worm. - : Landes Bioscience. - 2162-4046 .- 2162-4054. ; 2:1
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Journal article (peer-reviewed)abstract
- Cisplatin is an essential chemotherapeutic drug in the treatment of many cancers. Its use, however, is limited by the development of resistance in many tumors. The ability to re-sensitize resistant tumors could significantly strengthen cisplatin therapy in patients. Caenorhabditis elegans is a suitable model for studying the cytoplasmic role of cisplatin in tumor cells. We have previously shown that the ATPase ASNA-1 has similar roles as a factor governing cisplatin sensitivity in mammalian tumor cells and C. elegans. Here we study the endoplasmic reticulum (ER) resident chaperone ENPL-1/GRP94 and find that its depletion makes worms sensitive to cisplatin. Elevated ER stress levels in enpl-1 mutants is the likely cause of this sensitivity because a correlation can be made between cisplatin sensitivity and the high ER stress levels. We also find that asna-1 mutants have elevated unfolded protein response (UPR) activity and that the intrinsically cisplatin resistant wild-type worms become sensitive when ER stress is high. We conclude that enpl-1 is a cisplatin sensitizing factor and suggest that manipulation of its levels or of UPR activity will enhance the effects of cisplatin based cancer therapy.
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| 5. |
- Sheng, Ming, et al.
(author)
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Aberrant Fat Metabolism in Caenorhabditis elegans Mutants with Defects in the Defecation Motor Program
- 2015
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In: PLOS ONE. - : PLOS one. - 1932-6203. ; 10:4
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Journal article (peer-reviewed)abstract
- The molecular mechanisms by which dietary fatty acids are absorbed by the intestine, and the way in which the process is regulated are poorly understood. In a genetic screen for mutations affecting fat accumulation in the intestine of Caenorhabditis elegans, nematode worms, we have isolated mutations in the aex-5 gene, which encodes a Kex2/subtilisinfamily, Ca2+-sensitive proprotein convertase known to be required for maturation of certain neuropeptides, and for a discrete step in an ultradian rhythmic phenomenon called the defecation motor program. We demonstrate that aex-5 mutants have markedly lower steadystate levels of fat in the intestine, and that this defect is associated with a significant reduction in the rate at which labeled fatty acid derivatives are taken up from the intestinal lumen. Other mutations affecting the defecation motor program also affect steady-state levels of triglycerides, suggesting that the program is required per se for the proper accumulation of neutral lipids. Our results suggest that an important function of the defecation motor program in C. elegans is to promote the uptake of an important class of dietary nutrients. They also imply that modulation of the program might be one way in which worms adjust nutrient uptake in response to altered metabolic status.
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