1. |
- Botella Mascarell, Carmen, 1979, et al.
(författare)
-
EU FP7 INFSO-ICT-247223 ARTIST4G, D1.3 Innovative scheduling and cross-layer design techniques for interference avoidance
- 2011
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This document provides an overview of the proposed innovations and activities in Task 1.2 of Work Package 1 (WP1) of the ARTIST4G project, related to interference avoidance. Focus is on the technical approaches applicable at layer 2, which are grouped into five different classes of innovations related to clustering & user grouping, inter-cell interference coordination, coordinated scheduling, scheduling for joint processing and gametheory based scheduling. Descriptions of the proposed innovations are given including basic ideas, potential of performance, simulation results, realization options and possible implementation restrictions.
|
|
2. |
- Pons, Benoît J, et al.
(författare)
-
Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not
- 2019
-
Ingår i: PLOS ONE. - : Public Library Science. - 1932-6203. ; 14:3
-
Tidskriftsartikel (refereegranskat)abstract
- The Cytolethal Distending Toxin (CDT) is produced by many pathogenic bacteria. CDT is known to induce genomic DNA damage to host eukaryotic cells through its catalytic subunit, CdtB. CdtB is structurally homologous to DNase I and has a nuclease activity, dependent on several key residues. Yet some differences between various CdtB subunit activities, and discrepancies between biochemical and cellular data, have been observed. To better characterise the role of CdtB in the induction of DNA damage, we affinity-purified wild-type and mutants of CdtB, issued from E. coli and H. ducreyi, under native and denaturing conditions. We then compared their nuclease activity by a classic in vitro assay using plasmid DNA, and two different eukaryotic assays-the first assay where host cells were transfected with a plasmid encoding CdtB, the second assay where host cells were directly transfected with purified CdtB. We show here that in vitro nuclease activities are difficult to quantify, whereas CdtB activities in host cells can be easily interpreted and confirmed the loss of function of the catalytic mutant. Our results highlight the importance of performing multiple assays while studying the effects of bacterial genotoxins, and indicate that the classic in vitro assay should be complemented with cellular assays.
|
|