1. |
- BUCCIARELLI, SASKIA, et al.
(författare)
-
Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein
- 2016
-
Ingår i: The Journal of Physical Chemistry Letters. - : American Chemical Society (ACS). - 1948-7185. ; 7:9, s. 1610-1615
-
Tidskriftsartikel (refereegranskat)abstract
- Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.
|
|