| 1. |
- Linder, Astrid, 1959-, et al.
(författare)
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Open-Source Tools for Road User Safety Abessment from the VIRTUAL Project
- 2023
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Ingår i: TRA Lisbon 2022 Conference Proceedings Transport Research Arena. - : Elsevier. ; 72, s. 423-430
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Konferensbidrag (refereegranskat)abstract
- In the abessment of road user and vehicle occupant safety, physical testing is limited to a few scenarios. Virtual testing (VT) offers an opportunity to advance transport safety by introducing additional test cases. The objective of the VIRTUAL project is to provide tools such as finite element models, guidelines and a corresponding platform to foster the uptake of VT. A VT platform, OpenVT, has been established and provides open-source human body models (HBMs) of both an average female and male, seated and standing, as well as a seat, generic vehicle and tram front models. The tool chain from virtual to physical testing has been illustrated in the low severity impact case where the seat evaluation tool was developed. The newly established organisation OVTO will run the OpenVT platform in the future and govern the evolution of the results of the VIRTUAL project after its completion.
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| 2. |
- Neumann, Philipp-Alexander, et al.
(författare)
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Gut commensal bacteria and regional Wnt gene expression in the proximal versus distal colon
- 2014
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Ingår i: American Journal of Pathology. - : Elsevier. - 0002-9440 .- 1525-2191. ; 184:3, s. 592-9
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Tidskriftsartikel (refereegranskat)abstract
- Regional expression of Wingless/Int (Wnt) genes plays a central role in regulating intestinal development and homeostasis. However, our knowledge of such regional Wnt proteins in the colon remains limited. To understand further the effect of Wnt signaling components in controlling intestinal epithelial homeostasis, we investigated whether the physiological heterogeneity of the proximal and distal colon can be explained by differential Wnt signaling. With the use of a Wnt signaling-specific PCR array, expression of 84 Wnt-mediated signal transduction genes was analyzed, and a differential signature of Wnt-related genes in the proximal versus distal murine colon was identified. Several Wnt agonists (Wnt5a, Wnt8b, and Wnt11), the Wnt receptor frizzled family receptor 3, and the Wnt inhibitory factor 1 were differentially expressed along the colon length. These Wnt signatures were associated with differential epithelial cell proliferation and migration in the proximal versus distal colon. Furthermore, reduced Wnt/beta-catenin activity and decreased Wnt5a and Wnt11 expression were observed in mice lacking commensal bacteria, an effect that was reversed by conventionalization of germ-free mice. Interestingly, myeloid differentiation primary response gene 88 knockout mice showed decreased Wnt5a levels, indicating a role for Toll-like receptor signaling in regulating Wnt5a expression. Our results suggest that the morphological and physiological heterogeneity within the colon is in part facilitated by the differential expression of Wnt signaling components and influenced by colonization with bacteria.
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