| 1. |
- Johansson, Emil, et al.
(författare)
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Pentavalent Sialic Acid Conjugates Block Coxsackievirus A24 Variant and Human Adenovirus Type 37-Viruses That Cause Highly Contagious Eye Infections
- 2020
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Ingår i: ACS Chemical Biology. - : American Chemical Society (ACS). - 1554-8929 .- 1554-8937. ; 15:10, s. 2683-2691
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Tidskriftsartikel (refereegranskat)abstract
- Coxsackievirus A24 variant (CVA24v) and human adenovirus 37 (HAdV-37) are leading causative agents of the severe and highly contagious ocular infections acute hemorrhagic conjunctivitis and epidemic keratoconjunctivitis, respectively. Currently, neither vaccines nor antiviral agents are available for treating these diseases, which affect millions of individuals worldwide. CVA24v and HAdV-37 utilize sialic acid as attachment receptors facilitating entry into host cells. Previously, we and others have shown that derivatives based on sialic acid are effective in preventing HAdV-37 binding and infection of cells. Here, we designed and synthesized novel pentavalent sialic acid conjugates and studied their inhibitory effect against CVA24v and HAdV-37 binding and infection of human corneal epithelial cells. The pentavalent conjugates are the first reported inhibitors of CVA24v infection and proved efficient in blocking HAdV-37 binding. Taken together, the pentavalent conjugates presented here form a basis for the development of general inhibitors of these highly contagious ocular pathogens.
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| 2. |
- Johansson, Niklas, 1987-
(författare)
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On the Power of Quantum Computation: Oracles
- 2018
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Quantum computation solve some computational problems faster than the best-known alternative in classical computation. The evidence for this consists of examples where a quantum algorithm outperforms the best-known classical algorithm. A large body of these examples relies on oracle query complexity, where the performance (complexity) of the algorithms is measured by the number of times they need to access an oracle. Here, an oracle is usually considered to be a black box that computes a specific function at unit cost.However, the quantum algorithm is given access to an oracle with more structure than the classical algorithm. This thesis argues that the two oracles are so vastly different that comparing quantum and classical query complexity should not be considered evidence, but merely hints for a quantum advantage.The approach used is based on a model that can be seen as an approximation of quantum theory, but can be efficiently simulated on a classical computer. This model solves several oracular problems with the same performance as their quantum counterparts, showing that there is no genuine quantum advantage for these problems. This approach also clarifies the assumptions made in quantum computation, and which properties that can be seen as resources in these algorithms.
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| 3. |
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| 4. |
- Shu, Jiangpeng, 1987, et al.
(författare)
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Punching/Shear Strength of a full-scale tested bridge deck slab
- 2017
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Ingår i: IABSE Symposium, Vancouver, 2017. - Zürich, Switzerland : IABSE - International Association for Bridges and Structural Engineering. - 9783857481536 ; , s. 1750-1757, s. 1750-1757
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Konferensbidrag (refereegranskat)abstract
- For reinforced concrete (RC) slabs without shear reinforcement, shear and punching can be the governing failure mode at ultimate limit state if subjected to large concentrated loads. Punching of RC slabs without shear reinforcement has been a challenging problem in assessment based on current standards. To examine a previously developed enhanced analysis approach, this study was conducted by applying continuum FE analyses to a 55-year old RC bridge deck slab subjected to concentrated loads near the main girder in a field failure test. The influence of parameters such as boundary conditions, location of concentrated loads and shear force distribution were investigated.
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| 5. |
- Shu, Jiangpeng, 1987, et al.
(författare)
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Shear Capacity of a RC Bridge Deck Slab: Comparison between Multilevel Assessment and Field Test
- 2018
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Ingår i: Journal of Structural Engineering. - : American Society of Civil Engineers (ASCE). - 0733-9445 .- 1943-541X. ; 144:7
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Tidskriftsartikel (refereegranskat)abstract
- For reinforced concrete (RC) slabs without shear reinforcement, shear and punching can be the governing failure mode at the ultimate limit state if subjected to large concentrated loads. Shear and punching of RC slabs without shear reinforcement has been a challenging problem in assessment based on current standards. To examine a previously developed enhanced analysis approach, this study was conducted by applying a multilevel assessment strategy to a 55-year old RC bridge deck slab subjected to concentrated loads near the main girder in a field failure test. This strategy clearly provides the engineering community a framework for using successively improved structural analysis methods for enhanced assessment in a straightforward manner. The differences between analysis methods at different levels of assessment were discussed regarding one-way shear and punching shear behavior of the slab. The influences of parameters, such as boundary conditions, location of concentrated loads, and shear force distribution, were investigated.
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| 6. |
- Bergkvist, Linda, Assistant professor, 1979-, et al.
(författare)
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Redesigning Professional Development on Digital Transformation Using Andragogy as a Theoretical Lens
- 2023
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Ingår i: Proceedings of the 22nd European Conference on e-Learning - ECEL 2023. - : Academic Conferences and Publishing International Limited. - 9781914587900 - 9781914587917 ; , s. 25-32, s. 25-32
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Konferensbidrag (refereegranskat)abstract
- Regarding the ongoing digitalisation in the knowledge society, professional development seems more crucial than ever. The need for upskilling and reskilling is described as continuous lifelong learning, which must be combined and synchronised with the life of full-time working learners. Content, pedagogical models and instructional design in university courses are often created for students in Bachelor's and Master's programs instead of tailored for adults working full-time. This study describes and discusses andragogy as a potential knowledge base for redesigning professional development courses on digital transformation. Evaluations from two instances of a course for professionals on digital transformation showed that the course participants overall are satisfied with the course. However, only a few course participants take the exam to get credits. Therefore, the research question that guided this study was, "What redesign options for increased pass rates and learner satisfaction in professional development for adult learners can be identified using andragogy as a theoretical lens?" The course is on distance and contains four modules with synchronous and asynchronous learning activities, resulting in five European Credit Transfer System (ECTS). The empirical material consisted of course participants' check-in presentation before the course started, mid-term evaluation, final evaluations, and a learning diary containing 58 entries. The data was deductively analysed using the theory of andragogy as an analytical lens. The findings imply that instructors should put effort into how different parts of the course are connected, supporting learners' need to know. Further, to enhance the course participants' prior experience as a resource for learning by adding learning activities, they exchange experiences and examples with each other, adding to their learning process and networking. The pedagogic parts of understanding the theoretical course material could be split into pieces through exercises where the participants apply conceptual models and concepts to real-situation problems. The learning diary could help the participants align the new knowledge with their prior knowledge with a focus on professional roles and work situations. The identified redesign options create opportunities to increase pedagogical parts like readiness, orientation, and motivation to learn according to the current higher education system.
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| 7. |
- Bitenc, Jan, et al.
(författare)
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Concept and electrochemical mechanism of an Al metal anode - organic cathode battery
- 2020
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Ingår i: Energy Storage Materials. - : Elsevier BV. - 2405-8297 .- 2405-8289. ; 24, s. 379-383
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Tidskriftsartikel (refereegranskat)abstract
- Aluminum (Al) batteries are fundamentally a promising future post-Li battery technology. The recently demonstrated concept of an Al-graphite battery represents some significant progress for the technology, but the cell energy density is still very modest and limited by the quantity of the AlCl3 based electrolyte, as it relies on AlCl4- intercalation. For further progress, cathode materials capable of an electrochemical reaction with Al positively charged species are needed. Here such a concept of an Al metal anode - organic cathode battery based on anthraquinone (AQ) electrochemistry with a discharge voltage of 1.1 V is demonstrated. Further improvement of both the cell capacity retention and rate capability is achieved by nano-structured and polymerized cathodes. The intricate electrochemical mechanism is proven to be that the anthraquinone groups undergo reduction of their carbonyl bonds during discharge and become coordinated by AlCl2+ species. Altogether the Al metal anode - AQ cathode cell has almost the double energy density of the state-of-the-art Al-graphite battery.
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| 8. |
- Brodén, Karin, 1963-, et al.
(författare)
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Redesigning Technology-Enhanced Professional Development to Facilitate Lifelong Learning
- 2023
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Ingår i: ICERI2023 Proceedings. - Sevilla : IATED. - 9788409559428 ; , s. 604-611
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Konferensbidrag (refereegranskat)abstract
- Professional development is more crucial than ever, especially in supporting ongoing digitalisation in the knowledge society. The need for upskilling and reskilling is described as continuous lifelong learning, which must be combined and synchronised with the lives of full-time working learners. Content, pedagogical models and instructional design in university courses are often centred on students in their early twenties with little to no work experience instead of tailored for experienced professionals working full-time. One such course is the foundation for this study: a course on digitalisation and digital transformation targeting professionals with at least two years of work experience in digital transformation. Therefore, the research question that guided this study was: "How can a distance course for professionals be redesigned to facilitate lifelong learning?" The redesign aims to increase satisfaction with the course and the number of participants taking their exams. The course contains four modules with synchronous and asynchronous learning activities, resulting in 5 ECTS credits (the European Credit Transfer System). The analysed empirical material consists of course participants' check-in presentation before the course started, mid-term evaluation, final evaluation, and a learning diary containing 58 entries. The empirical material was inductively and iteratively analysed following the main steps of thematic analysis. Two of the researchers conducted the first steps of the analysis individually. The following steps were a joint coding procedure to reach a consensus. This part of the analysis included several discussions with the other researchers. The inductive analysis identified 70 initial codes that reflected issues related to course design, learning activities, course information, and instructions. The initial codes were clustered into seventeen more abstract themes based on similarities. The themes related to strengths and weaknesses/challenges with the course. The strengths were analysed as, among others, discussions with course participants in other domains, recordings introducing scientific articles, focused and direct instructions on the learning management system (LMS), and the course is held online at the same weekly time. Perceived challenges were, for example, reading scientific articles and understanding and transferring the content to their work situation, lack of correspondence among the modules, not all modules being published simultaneously on the LMS, and the general problem of mixing work and studies. Despite that, the material showed satisfied course participants, where only a few ended the course by taking the exam to get their credits, which is one of academia’s ways to quantify learning. Based on the data analysis, course and learning activities will be redesigned and developed to be tested and evaluated in a forthcoming course instance in autumn 2023.
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| 9. |
- Johansson, Emil, 1985-, et al.
(författare)
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Exploring divalent conjugates of 5-N-acetyl-neuraminic acid as inhibitors of coxsackievirus A24 variant (CVA24v) transduction
- 2022
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Ingår i: RSC Advances. - : Royal Society of Chemistry. - 2046-2069. ; 12:4, s. 2319-2331
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Tidskriftsartikel (refereegranskat)abstract
- Coxsackievirus A24 variant (CVA24v) is responsible for several outbreaks and two pandemics of the highly contagious eye infection acute hemorrhagic conjunctivitis (AHC). Currently, neither prevention (vaccines) nor treatments (antivirals) are available for combating this disease. CVA24v attaches to cells by binding Neu5Ac-containing glycans on the surface of cells which facilitates entry. Previously, we have demonstrated that pentavalent Neu5Ac conjugates attenuate CVA24v infection of human corneal epithelial (HCE) cells. In this study, we report on the structure-based design of three classes of divalent Neu5Ac conjugates, with varying spacer lengths, and their effect on CVA24v transduction in HCE cells. In relative terms, the most efficient class of divalent Neu5Ac conjugates are more efficient than the pentavalent Neu5Ac conjugates previously reported.
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| 10. |
- Johansson, Emil, 1985-, et al.
(författare)
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Exploring the effect of structure-based scaffold hopping on the inhibition of coxsackievirus a24v transduction by pentavalent n-acetylneuraminic acid conjugates
- 2021
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 22:16
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Tidskriftsartikel (refereegranskat)abstract
- Coxsackievirus A24 variant (CVA24v) is the primary causative agent of the highly contagious eye infection designated acute hemorrhagic conjunctivitis (AHC). It is solely responsible for two pandemics and several recurring outbreaks of the disease over the last decades, thus affecting millions of individuals throughout the world. To date, no antiviral agents or vaccines are available for combating this disease, and treatment is mainly supportive. CVA24v utilizes Neu5Ac-containing glycans as attachment receptors facilitating entry into host cells. We have previously reported that pentavalent Neu5Ac conjugates based on a glucose-scaffold inhibit CVA24v infection of human corneal epithelial cells. In this study, we report on the design and synthesis of scaffold-replaced pentavalent Neu5Ac conjugates and their effect on CVA24v cell transduction and the use of cryogenic electron microscopy (cryo-EM) to study the binding of these multivalent conjugates to CVA24v. The results presented here provide insights into the development of Neu5Ac-based inhibitors of CVA24v and, most significantly, the first application of cryo-EM to study the binding of a multivalent ligand to a lectin.
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