| 1. |
- Junaid, Sara, et al.
(författare)
-
Multifaceted biofuel production by microalgal isolates from Pakistan
- 2019
-
Ingår i: Biofuels, Bioproducts and Biorefining. - : WILEY. - 1932-104X .- 1932-1031. ; 13:5, s. 1187-1201
-
Tidskriftsartikel (refereegranskat)abstract
- Third-generation biofuels are currently considered to be the most resourceful medium for generating bioenergy. In the present study, microalgal strains were isolated from soil samples collected in Pakistan and characterized by 18S rRNA sequencing. The strains were identified as green algae Gloeocystis sp. MFUM-4, Sphaerocystis sp. MFUM-34, and Dictyochloropsis sp. MFUM-35. They were further studied for their potential to produce popular biofuels such as biodiesel, bioethanol, and biohydrogen. Under the test conditions, Gloeocystis sp. MFUM-4 emerged as the most suitable candidate, amongst the three new isolates, for biofuel production with a biodiesel production potential of 33.3% (w/v). Eight different environmental conditions were also tested to identify the most suitable condition for biohydrogen and bioethanol production using the newly isolated strains. Under light but in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), Gloeocystis recorded the highest capacity to produce both biohydrogen and bioethanol compared with the other strains that were examined.
|
|
| 2. |
- Khanna, Namita, et al.
(författare)
-
Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited: : Recent Progress and Future Prospects
- 2015
-
Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 16:5, s. 10537-10561
-
Forskningsöversikt (refereegranskat)abstract
- Cyanobacteria have garnered interest as potential cell factories for hydrogen production. In conjunction with photosynthesis, these organisms can utilize inexpensive inorganic substrates and solar energy for simultaneous biosynthesis and hydrogen evolution. However, the hydrogen yield associated with these organisms remains far too low to compete with the existing chemical processes. Our limited understanding of the cellular hydrogen production pathway is a primary setback in the potential scale-up of this process. In this regard, the present review discusses the recent insight around ferredoxin/flavodoxin as the likely electron donor to the bidirectional Hox hydrogenase instead of the generally accepted NAD(P)H. This may have far reaching implications in powering solar driven hydrogen production. However, it is evident that a successful hydrogen-producing candidate would likely integrate enzymatic traits from different species. Engineering the [NiFe] hydrogenases for optimal catalytic efficiency or expression of a high turnover [FeFe] hydrogenase in these photo-autotrophs may facilitate the development of strains to reach target levels of biohydrogen production in cyanobacteria. The fundamental advancements achieved in these fields are also summarized in this review.
|
|
| 3. |
|
|
| 4. |
- Khanna, Namita, et al.
(författare)
-
In vivo activation of an [FeFe] hydrogenase using synthetic cofactors
- 2017
-
Ingår i: Energy & Environmental Science. - : Royal Society of Chemistry (RSC). - 1754-5692 .- 1754-5706. ; 10:7, s. 1563-1567
-
Tidskriftsartikel (refereegranskat)abstract
- [FeFe] hydrogenases catalyze the reduction of protons, and oxidation of hydrogen gas, with remarkable efficiency. The reaction occurs at the H-cluster, which contains an organometallic [2Fe] subsite. The unique nature of the [2Fe] subsite makes it dependent on a specific set of maturation enzymes for its biosynthesis and incorporation into the apo-enzyme. Herein we report on how this can be circumvented, and the apo-enzyme activated in vivo by synthetic active site analogues taken up by the living cell.
|
|
| 5. |
- Khanna, Namita
(författare)
-
Perspectives on Algal Engineering for Enhanced Biofuel Production
- 2016
-
Ingår i: Algal Biorefinery. - Cham : Springer Publishing Company. - 9783319228136 - 9783319228129 ; , s. 73-101
-
Bokkapitel (refereegranskat)abstract
- Algae as photoautotrophs can trap the solar energy and convert it into usable form. Solar energy is the most abundant and ultimate energy source. The total amount of solar energy absorbed by the Earth’s surface is 1.74 × 105 terawatts (TW) (Bhattacharya S et al., Biochem Biotechnol 120:159–167, 2005), which is a tremendous amount as compared to the world’s energy consumption (~13 TW) (Walter JM et al., Curr Opin Biotechnol 21:265–270, 2010). Therefore, conversion of solar energy to fuels may constitute the most sustainable way to solve the energy crisis.
|
|
| 6. |
- Khetorn, Wanthanee, et al.
(författare)
-
Metabolic and genetic engineering of cyanobacteria for enhanced hydrogen production
- 2013
-
Ingår i: Biofuels. - : Informa UK Limited. - 1759-7269 .- 1759-7277. ; 4:5, s. 535-561
-
Tidskriftsartikel (refereegranskat)abstract
- There is an urgent need to develop sustainable solutions to convert solar energy into energy carriers used in the society. In addition to solar cells generating electricity, there are several options to generate solar fuels with molecular hydrogen (H2) being an interesting and promising option. Native and engineered cyanobacteria have been used as model systems to examine, develop and demonstrate photobiological hydrogen production. In the present review we present and discuss recent progress with respect to (i) native biological systems to generate hydrogen, (ii) metabolic modulations, and (iii) genetic engineering of metabolic pathways, as well as the (iv) introduction of custom-designed, non-native enzymes and complexes for enhanced hydrogen production in cyanobacteria. In conclusion, metabolic and genetic engineering of native cyanobacterial hydrogen metabolism can significantly increase the hydrogen production, and introduction of custom-designed non-native capacities open up new possibilities to further enhance cyanobacterial based hydrogen production.
|
|
| 7. |
- Lindblad, Peter, et al.
(författare)
-
CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels
- 2019
-
Ingår i: Algal Research. - : Elsevier. - 2211-9264. ; 41
-
Forskningsöversikt (refereegranskat)abstract
- CyanoFactory, Design, construction and demonstration of solar biofuel production using novel (photo) synthetic cell factories, was an R&D project developed in response to the European Commission FP7-ENERGY-2012-1 call "Future Emerging Technologies" and the need for significant advances in both new science and technologies to convert solar energy into a fuel. CyanoFactory was an example of "purpose driven" research and development with identified scientific goals and creation of new technologies. The present overview highlights significant outcomes of the project, three years after its successful completion. The scientific progress of CyanoFactory involved: (i) development of a ToolBox for cyanobacterial synthetic biology; (ii) construction of DataWarehouse/Bioinformatics web-based capacities and functions; (iii) improvement of chassis growth, functionality and robustness; (iv) introduction of custom designed genetic constructs into cyanobacteria, (v) improvement of photosynthetic efficiency towards hydrogen production; (vi) biosafety mechanisms; (vii) analyses of the designed cyanobacterial cells to identify bottlenecks with suggestions on further improvements; (viii) metabolic modelling of engineered cells; (ix) development of an efficient laboratory scale photobioreactor unit; and (x) the assembly and experimental performance assessment of a larger (1350 L) outdoor flat panel photobioreactor system during two seasons. CyanoFactory - Custom design and purpose construction of microbial cells for the production of desired products using synthetic biology - aimed to go beyond conventional paths to pursue innovative and high impact goals. CyanoFactory brought together ten leading European partners (universities, research organizations and enterprises) with a common goal - to develop the future technologies in Synthetic biology and Advanced photobioreactors.
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
