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- Glasbey, JC, et al.
(författare)
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- 2021
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swepub:Mat__t
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- Echelmeier, A., et al.
(författare)
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Segmented flow generator for serial crystallography at the European X-ray free electron laser
- 2020
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Ingår i: Nature Communications. - : Nature Research. - 2041-1723. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.
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- Kirkwood, S., et al.
(författare)
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Polar mesosphere winter echoes by ESRAD, EISCAT and lidar
- 2002
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- The ESRAD 52 MHz MST radar (67° 53 ‘ N, 21 ° 06 ‘ E) has observed thin layers of enhanced radar echoes in the winter mesosphere during several recent solar proton events. The detection of these polar mesosphere winter echoes (PMWE) is generally found to correlates well with low values of λ (the ratio of negative ion density to electron density). However PMWE are found to persist for values of λ up to ~100. Present knowledge of the nature of neutral turbulence in the winter mesosphere suggests that such turbulence cannot generate electron density fluctuations with scale-sizes as short as the 3 m needed to produce radar echoes at 52 MHz. This is particularly true as λ increases to ~100. Joint observations from ESRAD and the EISCAT 224 MHz radar suggest that PMWE is also detectable at 67 cm scale-sizes, further increasing the difficulty in explaining the echoes by neutral turbulence. Joint observations from ESRAD and lidar are also inconsistent with the expected behaviour of turbulence. Together with results concerning the thickness, echo aspect-sensitivity and echo spectral-width of the PMWE, these observation leads to the conclusion that the layers cannot be explained by turbulence alone. A role for charged aerosols in creating PMWE is proposed. The presence of aerosols is supported by the lidar observations.
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