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Träfflista för sökning "WFRF:(Kjærbølling I.) "

Sökning: WFRF:(Kjærbølling I.)

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1.
  • Siedler, S., et al. (författare)
  • Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production
  • 2017
  • Ingår i: ACS Synthetic Biology. - : American Chemical Society (ACS). - 2161-5063. ; 6:10, s. 1860-1869
  • Tidskriftsartikel (refereegranskat)abstract
    • Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
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2.
  • Siedler, S., et al. (författare)
  • High throughput droplet sorting of yeast for p-Coumaric acid production detected by co-encapsulated E. coli biosensor bacteria
  • 2016
  • Ingår i: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. - : Chemical and Biological Microsystems Society. - 9780979806490 ; , s. 551-552
  • Konferensbidrag (refereegranskat)abstract
    • We present the optimization of E. coli biosensor bacteria and demonstrate their use to detect and sort co-encapsulated S. cerevisiae yeast cells by their p-Coumaric acid (pCA) production in picoliter microfluidic droplets at high throughput. This strategy allows us to enrich pCA producing cell factories by the cell permeable product pCA using a biosensor in a separate cell.
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  • Resultat 1-2 av 2

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