| 1. |
- Callaway, EM, et al.
(author)
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A multimodal cell census and atlas of the mammalian primary motor cortex
- 2021
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In: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 598:7879, s. 86-102
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Journal article (peer-reviewed)abstract
- Here we report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties and cellular resolution input–output mapping, integrated through cross-modal computational analysis. Our results advance the collective knowledge and understanding of brain cell-type organization1–5. First, our study reveals a unified molecular genetic landscape of cortical cell types that integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a consensus taxonomy of transcriptomic types and their hierarchical organization that is conserved from mouse to marmoset and human. Third, in situ single-cell transcriptomics provides a spatially resolved cell-type atlas of the motor cortex. Fourth, cross-modal analysis provides compelling evidence for the transcriptomic, epigenomic and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types. We further present an extensive genetic toolset for targeting glutamatergic neuron types towards linking their molecular and developmental identity to their circuit function. Together, our results establish a unifying and mechanistic framework of neuronal cell-type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties.
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| 2. |
- Wang, H. Y., et al.
(author)
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Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol
- 2020
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In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 11
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Journal article (peer-reviewed)abstract
- Phenol is a ubiquitous pollutant and can contaminate natural water resources. Hence, the removal of phenol from wastewater is of significant importance. A series of biological methods were used to remove phenol based on the natural ability of microorganisms to degrade phenol, but the tolerance mechanism of phenol-degraded strains to phenol are not very clear. Morphological observation on Candida tropicalis showed that phenol caused the reactive oxygen species (ROS) accumulation, damaging the mitochondrial and the endoplasmic reticulum. On the basis of transcriptome data and cell wall susceptibility analysis, it was found that C. tropicalis prevented phenol-caused cell damage through improvement of cell wall resistance, maintenance of high-fidelity DNA replication, intracellular protein homeostasis, organelle integrity, and kept the intracellular phenol concentration at a low level through cell-wall remodeling and removal of excess phenol via MDR/MXR transporters. The knowledge obtained will promote the genetic modification of yeast strains in general to tolerate the high concentrations of phenol and improve their efficiency of phenol degradation.
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| 3. |
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| 4. |
- Liu, Y., et al.
(author)
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Increases in China's wind energy production from the recovery of wind speed since 2012
- 2022
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In: Environmental Research Letters. - : IOP Publishing. - 1748-9326. ; 17:11
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Journal article (peer-reviewed)abstract
- China has realized a 56-fold increase in installed wind capacity, from 5.9 GW in 2007 to 328 GW in 2021. In addition to increasing installed capacity, plans to substantially increase wind energy production for climate change mitigation also depend on future wind speeds, which strongly influences the efficiencies of installed turbines within individual wind farms. A reversal in globally decreasing wind speeds over several decades has been reported previously. However, subsequent studies using other data sources reported only a slight increase or no reversal in China. These uncertainties regarding China's wind energy production hamper estimates of wind energy production potential. Here, our analysis of quality-controlled wind speed measurements from in-situ stations shows that the wind speed decline in China reversed significantly since 2012 (P < 0.001), but with substantial spatio-temporal variability. We further estimated the capacity factor (CF) growth and the wind power gain solely associated with the changes in wind speed ranges from 31.6 to 56.5 TWh yr(-1) based on the 2019 installed capacity. This estimate explains 22.0%-39.3% of the rapid increase in wind generation CF in China during 2012-2019. The result implies that the site selection of wind farms should consider both current wind situation and future wind speed trends. Further studies are needed to understand the driving factor of wind speed recovery in support of the wind energy industry.
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| 5. |
- Pi, X. H., et al.
(author)
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Chlorophyll-a concentrations in 82 large alpine lakes on the Tibetan Plateau during 2003-2017: temporal-spatial variations and influencing factors
- 2021
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In: International Journal of Digital Earth. - : Informa UK Limited. - 1753-8947 .- 1753-8955. ; 14:6, s. 714-735
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Journal article (peer-reviewed)abstract
- As essential parts of the unique ecosystem of Tibetan Plateau (TP), the sizes and associated physical properties of alpine lakes have long been investigated. However, little is known about one of the most critical biogeochemical properties, i.e. the Chlorophyll-a (Chl-a) concentrations. Here, for the first time, we presented a comprehensive investigation of the temporal-spatial variations in Chl-a in 82 lakes (>50 km(2)) across the entire TP region, based on MODIS observations in the period of 2003-2017. The results showed that the 82 lakes exhibited an average long-term mean Chl-a of 3.3 +/- 4.3 mg m(-3), with high Chl-a lakes concentrated in the eastern and southern inner TP basin and northeastern parts of the TP. An interannual trend analysis revealed that lakes exhibiting (significantly) decreasing Chl-a trends and (significantly) increasing Chl-a trends were comparable in numbers but differed in distribution patterns. A correlation analysis indicated that at least 70% of the interannual variability in Chl-a values of lakes was significantly correlated with one of the four environmental factors (wind speed, ice cover duration, lake water surface temperature and surface runoff) and lake size. In addition, glacier meltwater tended to reduce lake Chl-a while salinity levels showed minor influences.
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| 6. |
- Zhang, X., et al.
(author)
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Revisiting Lightning Activity and Parameterization Using Geostationary Satellite Observations
- 2021
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In: Remote Sensing. - : MDPI AG. - 2072-4292. ; 13:19
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Journal article (peer-reviewed)abstract
- The Geostationary Lightning Mapper (GLM) on the Geostationary Operational Environmental Satellite 16 (GOES-16) detects total lightning continuously, with a high spatial resolution and detection efficiency. Coincident data from the GLM and the Advanced Baseline Imager (ABI) are used to explore the correlation between the cloud top properties and flash activity across the continental United States (CONUS) sector from May to September 2020. A large number of collocated infrared (IR) brightness temperature (TBB), cloud top height (CTH) and lightning data provides robust statistics. Overall, the likelihood of lightning occurrence and high flash density is higher if the TBB is colder than 225 K. The higher CTH is observed to be correlated with a larger flash rate, a smaller flash size, stronger updraft, and larger optical energy. Furthermore, the cloud top updraft velocity (w) is estimated based on the decreasing rate of TBB, but it is smaller than the updraft velocity of the convective core. As a result, the relationship between CTH and lightning flash rate is investigated independently of w over the continental, oceanic and coastal regimes in the tropics and mid-latitudes. When the CTH is higher than 12 km, the flash rates of oceanic lightning are 38% smaller than those of both coastal and continental lightning. In addition, it should be noted that more studies are necessary to examine why the oceanic lightning with low clouds (CTH < 8 km) has higher flash rates than lightning over land and coast. Finally, the exponents of derived power relationship between CTH and lightning flash rate are smaller than four, which is underestimated due to the GLM detection efficiency and the difference between IR CTH and 20 dBZ CTH. The results from combining the ABI and GLM products suggest that merging multiple satellite datasets could benefit both lightning activity and parameterization studies, although the parallax corrections should be considered.
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| 7. |
- Kuang, H. Y., et al.
(author)
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Acupuncture and Clomiphene Citrate for Live Birth in Polycystic Ovary Syndrome: Study Design of a Randomized Controlled Trial
- 2013
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In: Evidence-Based Complementary and Alternative Medicine. - : Hindawi Limited. - 1741-427X .- 1741-4288.
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Journal article (peer-reviewed)abstract
- Acupuncture is an alternative therapy to induce ovulation in women with polycystic ovary syndrome (PCOS), but there is no study reporting the live birth rate following ovulation induction by acupuncture or its potential as an adjuvant treatment to clomiphene citrate (CC). We assess the efficacy of acupuncture with or without CC in achieving live births among 1000 PCOS women in Mainland China. This paper reports the methodology of an ongoing multicenter randomized controlled trial. The randomization scheme is coordinated through the central mechanism and stratified by the participating sites. Participants will be randomized into one of the four treatment arms: (A) true acupuncture and CC, (B) control acupuncture and CC, (C) true acupuncture and placebo CC, and (D) control acupuncture and placebo CC. To ensure the quality and integrity of the trial we have developed a unique multinational team of investigators and Data and Safety Monitoring Board. Up to the end of April 2013, 326 subjects were recruited. In conclusion, the success of this trial will allow us to evaluate the additional benefit of acupuncture beyond the first line medicine for fertility treatment in PCOS women in an unbiased manner.
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| 8. |
- Qi, W., et al.
(author)
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Divergent and Changing Importance of Glaciers and Snow as Natural Water Reservoirs in the Eastern and Southern Tibetan Plateau
- 2022
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In: Journal of Geophysical Research-Atmospheres. - : American Geophysical Union (AGU). - 2169-897X .- 2169-8996. ; 127:7
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Journal article (peer-reviewed)abstract
- Glaciers and snow are natural water reservoirs in the Tibetan Plateau (TP), affecting ecosystems, water and food security, and more than one billion downstream people. Meltwater volumes are traditionally estimated using the degree-day concept considering only air temperature, which cannot consider the influence from downward solar and longwave radiation, humidity, wind and resultant turbulent heat fluxes. Here, we used a physically based energy budget approach considering the full energy balance in seven large river basins in the eastern and southern TP. For 1982-2011, the estimated average glacier melt was 0.32 +/- 0.007 m water equivalent/yr with large spatial variability. Air temperature, downward longwave radiation, humidity, and wind speed influenced the overall glacier melt trend, and glacier melt accelerated with a rate of 0.42 mm/yr. Yet, downward shortwave radiation played an additional role in influencing the glacier melt rate fluctuation. On average, snow and glacier melt contributed 17.6% of annual river discharge during 1982-2011, including 10.0% from snow and 7.6% from glaciers. The highest and lowest relative melt contributions were in the Yarlung Tsangpo and Yalong Rivers, respectively. Mainly due to decreasing snow melt, glacier and snow melt contributions to discharge would decrease to 11.9% during 2021-2050 under the extreme climate scenario (RCP8.5), and the greatest change in the relative contributions would occur in the Upper Nu River (-9.2%). These findings indicate the divergent and changing importance of glaciers and snow as natural water reservoirs, potentially affecting socioeconomic development and adaptation to climate change in South, Southeast, and East Asia.
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