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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Luo, Jiaxin, et al.
(författare)
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Solar cells sensitized by donor-linked concerted companion dyes
- 2023
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Ingår i: Journal of Materials Chemistry C. - : ROYAL SOC CHEMISTRY. - 2050-7526 .- 2050-7534. ; 11:16, s. 5450-5460
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Tidskriftsartikel (refereegranskat)abstract
- Recently, concerted companion (CC) dyes have been developed by covalently linking the acceptors of organic and porphyrin dye units. Herein, a new class of CC dyes XW85 and XW86 have been designed by linking the donors of porphyrin and organic dye units with C6H12 and C12H24 chains, respectively. The DSSCs of XW85 based on the I-3(-)/I- electrolyte show significant J(SC) (17.20 mA cm(-2)) and PCE (8.96%), and XW86 exhibits higher J(SC) (18.55 mA cm(-2)) and PCE (9.76%), which are also higher than those of the corresponding cosensitization systems. However, the PCEs for XW85 and XW86 are lower than that of the acceptor-linked reference dye XW76 despite the obviously larger dye adsorption amounts. Desorption studies reveal that the CC dyes may be either double-anchored or single-anchored, with the double/single anchoring ratios lying in a sequence of XW85 (1.31) < XW86 (1.88) < XW76 (6.34), consistent with that of increasing PCE. These observations indicate that the non-adsorbed sub-dye unit cannot effectively contribute to electron injection, and thus relatively large mono-anchoring proportions for XW85 and XW86 result in their relatively low J(SC), and the difference between XW85 and XW86 indicates that a longer linking chain is beneficial for partially alleviating the unfavorable single anchoring, resulting in superior performance. The results indicate that the photovoltaic behavior for the CC dyes may be further enhanced by avoiding the unfavorable alignment of the two carboxyl groups in opposite directions and thus simultaneously anchoring the two carboxyl groups, which may be realized through more rational molecular design.
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| 4. |
- Peng, Jiaxin, et al.
(författare)
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A Deep Neural Network Accelerator using Residue Arithmetic in a Hybrid Optoelectronic System
- 2022
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Ingår i: ACM Journal on Emerging Technologies in Computing Systems. - New York : Association for Computing Machinery (ACM). - 1550-4832 .- 1550-4840. ; 18:4
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Tidskriftsartikel (refereegranskat)abstract
- The acceleration of Deep Neural Networks (DNNs) has attracted much attention in research. Many critical real-time applications benefit from DNN accelerators but are limited by their compute-intensive nature. This work introduces an accelerator for Convolutional Neural Network (CNN), based on a hybrid optoelectronic computing architecture and residue number system (RNS). The RNS reduces the optical critical path and lowers the power requirements. In addition, the wavelength division multiplexing (WDM) allows high-speed operation at the system level by enabling high-level parallelism. The proposed RNS compute modules use one-hot encoding, and thus enable fast switching between the electrical and optical domains. We propose a new architecture that combines residue electrical adders and optical multipliers as the matrix-vector multiplication unit. Moreover, we enhance the implementation of different CNN computational kernels using WDM-enabled RNS based integrated photonics. The area and power efficiency of the proposed accelerator are 0.39 TOPS/s/mm(2) and 3.22 TOPS/s/W, respectively. In terms of computation capability, the proposed chip is 12.7x and 4.02x better than other optical implementation and memristor implementation, respectively. Our experimental evaluation using DNN benchmarks illustrates that our architecture can perform on average more than 72 times faster than GPU under the same power budget. © 2023 ACM, Inc.
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