| 1. |
- Biel, Lena, et al.
(författare)
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ECG analysis : a new approach in human identification
- 2001
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In this paper a new approach in human identification is investigated, For this purpose, a standard 12-lead electrocardiogram (ECG) recorded during rest is used. Selected features extracted from the ECG are used to identify a person in a predetermined group. Multivariate analysis is used for the identification task. Experiments show that it is possible to identify a person by features extracted from one lead only. Hence, only three electrodes have to be attached on the person to be identified. This makes the method applicable without too much effort.
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| 2. |
- Dahl, JoAnne, et al.
(författare)
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Behavioral medicine treatment in chronic constipation with paradoxical anal sphincter contraction
- 1991
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Ingår i: Diseases of the Colon & Rectum. - 0012-3706 .- 1530-0358. ; 34:9, s. 769-776
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Tidskriftsartikel (refereegranskat)abstract
- Nine women and five children with severe chronic constipation received behavioral medicine therapy. Before treatment, all patients had a paradoxical contraction of the external anal sphincter at defecation attempts as demonstrated with electromyography and/or anorectal manometry. An electromyographic biofeedback device connected to an anal probe was used for the training that was performed on a regular toilet seat during five 1-hour sessions. Thirteen of the patients improved considerably and could learn to defecate spontaneously, and the use of laxatives ceased or diminished. Simultaneously with improvement, the paradoxical anal contraction disappeared. The results remained after 6 months, although two of the patients had received booster sessions of biofeedback training during follow-up.
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| 3. |
- Lindberg, Uno, et al.
(författare)
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Isolation and Characterization of Adenovirus Messenger Ribonucleic Acid in Productive Infection
- 1972
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Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 10:5, s. 909-919
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Tidskriftsartikel (refereegranskat)abstract
- Messenger ribonucleic acid (mRNA) from cells productively infected with adenovirus type 2 was isolated by affinity chromatography on polyuridylic acid [poly (U)] bound to Sepharose. At least 90% of the polyadenylic acid [poly (A)]-containing polysomal mRNA was retained by the poly (U) Sepharose and thus separated from more than 95% of the ribosomal RNA and transfer RNA. In these experiments, 65% of the early (3 to 5 hr postinfection) and 85% of the late (14 to 16 hr postinfection) virus-specific RNA was retained by the poly (U) Sepharose. Early in the infection 18%, and late in the infection more than 95%, of the poly (A)-containing fraction, eluted from the poly (U) Sepharose with 90% formamide, was adenovirus-specific, as shown by exhaustive hybridization. Different patterns, containing several distinct species of viral mRNA, were detected early and late in the infectious cycle. No distinct viral mRNA lacking poly (A) was discovered.
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| 4. |
- Loskog, Angelica, et al.
(författare)
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Human urinary bladder carcinomas express adenovirus attachment and internalization receptors
- 2002
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Ingår i: Gene Therapy. - : Springer Science and Business Media LLC. - 0969-7128 .- 1476-5462. ; 9:9, s. 547-553
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Tidskriftsartikel (refereegranskat)abstract
- The use of adenoviral vectors as potent gene delivery systems requires expression of the Coxsackievirus/adenovirus receptor (CVADR) on the target cell surface. This receptor is important for virus attachment to the cell surface. For effective internalization of the vector into the target cell the integrins alpha(v)beta(3) and/or alpha(v)beta(5) are needed. Since there have been reports of loss of CVADR in bladder cancer cell lines, we wanted to investigate the expression of this receptor in bladder carcinoma biopsies. Surgical biopsies, as well as five human bladder cancer cell lines, were analyzed for expression of CVADR, the integrins alpha(v)beta(3) and alpha(v)beta(5) and MHC class I. Further, we studied the ability to transduce these cell lines using adenoviral vectors. Immunohistochemistry revealed that all biopsies (27/27) were positive for CVADR. Some variation in expression was evident, and superficially growing tumors stained more strongly than invasive ones. Most human tumors expressed the integrin alpha(v)beta(5) (14/24), whereas integrin alpha(v)beta(3) was less frequently seen (3/20). The established cell lines were efficiently transduced with adenoviral vectors, and transduction could be reduced with anti-CVADR antibodies. The abundance of appropriate viral receptors on tumor biopsy cells is a further argument for using adenoviral vectors in gene therapy of bladder cancer.
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| 5. |
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| 6. |
- Philipson, Lennart, 1952-
(författare)
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The electromyographic signal used for control of upper extremity prostheses and for quantification of motor blockade during epidural anaesthesia
- 1987
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- A contribution of biomedical research engineering has been the development of detection and analysis methods for the electromyographic (EMG) signal and its application to health care problems. The present thesis describes the use of EMG signals for two such applications: Aid in the control of multifunctional upper limb prostheses and for quantification of motor blockade during epidural anaesthesia. A myoelectric control system operated by synergies from the EMG signals generated by two muscles is presented in papers I, II, and III. Results from paper I suggest that microprocessor based control systems for upper extremity prostheses could emulate all previous controllers based on discrete components. The study presented in paper II includes a graphic presentation of the EMG signal to this computer based control system and a visualization of the control algorithm and prosthetic movement. A series of single subject case studies of amputees showed successful operation of the prosthesis using the described system. Paper III presents a method for the quantification of success rate and response time for multiple-state control systems when applied to a group of non-disabled volunteers. Results showed that an increase in complexity of the control system was correlated to a decrease in performance. A prosthesis with four myoelectrically controlled joint movements was shown to be operable with a mean control accuracy of 53 percent subsequent to introductory training. In paper IV a presentation is made of the relationships between the isometric force exerted by the biceps brachii and six different quantities of the EMG simultaneously detected with bipolar surface and concentric needle electrodes. Results implied that amplitude sensitive parameters may be the best descriptors of isometric force from 0 to 100 percent of maximal voluntary contraction. In addition it was shown that bipolar surface electrodes may be superior to concentric needle electrodes for detection of EMG as a substitute measure of force. Paper V describes the use of EMG signals as a method for quantifying motor blockade during epidural anaesthesia. Surface EMG was detected from muscles with innervations from spinal levels T7, T9, T11 and L2-L4. Quantification of the recorded EMG during onset, duration and regression of anaesthesia appeared to enable quantitative mapping of both the temporal and spatial motor blockade behavior of the drug used in epidural anaesthesia.It is concluded that the applications of detected and quantified EMG signals as presented in this thesis may lead to improvements both in the areas of prosthesis operation for amputees and for detection of motor blockade during epidural anaesthesia.
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| 7. |
- Ranjbar, Parivash, et al.
(författare)
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Auditive identification of signal-processed environmental sounds : monitoring the environment
- 2008
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Ingår i: International Journal of Audiology. - London : Taylor & Francis. - 1499-2027 .- 1708-8186. ; 47:12, s. 724-736
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Tidskriftsartikel (refereegranskat)abstract
- The goal of the present study was to compare six transposing signal-processing algorithms based on different principles (Fourier-based and modulation based), and to choose the algorithm that best enables identification of environmental sounds, i.e. improves the ability to monitor events in the surroundings. Ten children (12-15 years) and 10 adults (21-33 years) with normal hearing listened to 45 representative environmental (events) sounds processed using the six algorithms, and identified them in three different listening experiments involving an increasing degree of experience. The sounds were selected based on their importance for normal hearing and deaf-blind subjects. Results showed that the algorithm based on transposition of 1/3 octaves (fixed frequencies) with large bandwidth was better (p<0.015) than algorithms based on modulation. There was also a significant effect of experience (p<0.001). Adults were significantly (p<0.05) better than children for two algorithms. No clear gender difference was observed. It is concluded that the algorithm based on transposition with large bandwidth and fixed frequencies is the most promising for development of hearing aids to monitor environmental sounds.
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| 8. |
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| 9. |
- Sollerbrant, Kerstin, et al.
(författare)
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The Coxsackievirus and adenovirus receptor (CAR) forms a complex with the PDZ domain-containing protein ligand-of-numb protein-X (LNX)
- 2003
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 278:9, s. 7439-7444
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Tidskriftsartikel (refereegranskat)abstract
- The Coxsackievirus and adenovirus receptor (CAR) functions as a virus receptor, but its primary biological function is unknown. A yeast two-hybrid screen was used to identify Ligand-of-Numb protein-X (LNX) as a binding partner to the intracellular tail of CAR. LNX harbors several protein-protein interacting domains, including four PDZ domains, and was previously shown to bind to and regulate the expression level of the cell-fate determinant Numb. CAR was able to bind LNX both in vivo and in vitro. Efficient binding to LNX required not only the consensus PDZ domain binding motif in the C terminus of CAR but also upstream sequences. The CAR binding region in LNX was mapped to the second PDZ domain. CAR and LNX were also shown to colocalize in vivo in mammalian cells. We speculate that CAR and LNX are part of a larger protein complex that might have important functions at discrete subcellular localizations in the cell.
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| 10. |
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