| 1. |
- Bernal, Ximena E., et al.
(författare)
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Empowering Latina scientists
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 363:6429, s. 825-826
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
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| 3. |
- Garcia, E., et al.
(författare)
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Robustness of Paracentrotus lividus larval and post-larval development to pH levels projected for the turn of the century
- 2015
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Ingår i: Marine Biology. - : Springer Science and Business Media LLC. - 0025-3162 .- 1432-1793. ; 162:10, s. 2047-2055
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Tidskriftsartikel (refereegranskat)abstract
- Ocean acidification is causing changes to the chemistry and biology of the marine environment, in ways that we are only just beginning to understand. Growing evidences demonstrate that ocean acidification can influence the survival, growth, development, and physiology of marine invertebrates. Here, we assessed the impact of ocean acidification on the sea urchin Paracentrotus lividus larval development (from embryos exposed to experimental conditions from the 24 h gastrula stage to 35 days) and settlement. Samples were collected from the Canary Islands (28A degrees 24'N, 16A degrees 18'W) in March 2012. Three pH treatments were tested: (1) pH 8.1, the present average pH; (2) pH 7.7, the average predicted for the year 2100, but already experienced in the natural environment during extremes of variability; and (3) pH 7.4, predicted extremes of natural variability by 2100. The mortality rate was significantly increased by 40 % at the lowest pH. Time required by larvae to achieve each developmental stage (from gastrula to competent stage) was decreased at pH 7.7 (larval development speed increased by 18 %), but larval morphology at a given size did not differ from the other pH treatments. Settlement was delayed by 8 days at pH 7.7 compared to pH 8.1, and no settlement was observed at pH 7.4. Overall, only sublethal effects were observed in larvae exposed to pH 7.7, while pH 7.4 induced both lethal and sublethal effects. Our results support the hypothesis that P. lividus is robust to survive in an environment with the present natural variation. However, the species is sensitive to extreme levels of pH that are predicted within the next 90 years.
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| 4. |
- Singh, Vinayak, et al.
(författare)
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Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria.
- 2017
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Ingår i: Molecular Microbiology. - : Wiley. - 0950-382X .- 1365-2958. ; 103:1, s. 13-25
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Tidskriftsartikel (refereegranskat)abstract
- There is an urgent need to discover new anti-tubercular agents with novel mechanisms of action in order to tackle the scourge of drug-resistant tuberculosis. Here, we report the identification of such a molecule - an AminoPYrimidine-Sulfonamide (APYS1) that has potent, bactericidal activity against M. tuberculosis. Mutations in APYS1-resistant M. tuberculosis mapped exclusively to wag31, a gene that encodes a scaffolding protein thought to orchestrate cell elongation. Recombineering confirmed that a Gln201Arg mutation in Wag31 was sufficient to cause resistance to APYS1, however, neither overexpression nor conditional depletion of wag31 impacted M. tuberculosis susceptibility to this compound. In contrast, expression of the wildtype allele of wag31 in APYS1-resistant M. tuberculosis was dominant and restored susceptibility to APYS1 to wildtype levels. Time-lapse imaging and scanning electron microscopy revealed that APYS1 caused gross malformation of the old pole of M. tuberculosis, with eventual lysis. These effects resembled the morphological changes observed following transcriptional silencing of wag31 in M. tuberculosis. These data show that Wag31 is likely not the direct target of APYS1, but the striking phenotypic similarity between APYS1 exposure and genetic depletion of Wag31 in M. tuberculosis suggests that APYS1 might indirectly affect Wag31 through an as yet unknown mechanism.
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