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Sökning: WFRF:(STENDAHL P)

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  • Favre, Cécile J., et al. (författare)
  • Organization of Ca2+ stores in myeloid cells: association of SERCA2b and the type-1 inositol-1,4,5-trisphosphate receptor
  • 1996
  • Ingår i: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 316:1, s. 137-142
  • Tidskriftsartikel (refereegranskat)abstract
    • In this study, we have analysed the relationship between Ca2+ pumps and Ins(1,4,5)P3-sensitive Ca2+ channels in myeloid cells. To study whether sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA)-type Ca2+-ATPases are responsible for Ca2+ uptake into Ins(1,4,5)P3-sensitive Ca2+ stores, we used the three structurally unrelated inhibitors thapsigargin, 2,5-di-t-butylhydroquinone and cyclopiazonic acid. In HL-60 cells, all three compounds precluded formation of the phosphorylated intermediate of SERCA-type Ca2+-ATPases. They also decreased, in parallel, ATP-dependent Ca2+ accumulation and the amount of Ins(1,4,5)P3-releasable Ca2+. Immunoblotting with subtype-directed antibodies demonstrated that HL-60 cells contain the Ca2+ pump SERCA2 (subtype b), and the Ca2+-release-channel type-1 Ins(1,4,5)P3 receptor. In subcellular fractionation studies, SERCA2 and type-1 Ins(1,4,5)P3 receptor co-purified. Immunofluorescence studies demonstrated that both type-1 Ins(1,4,5)P3 receptor and SERCA2 were evenly distributed throughout the cell in moving neutrophils. During phagocytosis both proteins translocated to the periphagosomal space. Taken together, our results suggest that in myeloid cells (i) SERCA-type Ca2+-ATPases function as Ca2+ pumps of Ins(1,4,5)P3-sensitive Ca2+ stores, and (ii) SERCA2 and type-1 Ins(1,4,5)P3 receptor reside either in the same or two tightly associated subcellular compartments.
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  • Hammar, T., et al. (författare)
  • Life cycle assessment of climate impact of bioenergy from a landscape
  • 2017
  • Ingår i: European Biomass Conference and Exhibition Proceedings 2017. - : ETA-Florence Renewable Energies. ; , s. 1493-1497
  • Konferensbidrag (refereegranskat)abstract
    • Bioenergy is a renewable energy source that can replace fossil energy sources in order to decrease greenhouse gas emissions. Assessing the climate impact of bioenergy systems involves methodological choices that may influence the result. Choice of climate metric is one example that has been discussed in several papers recently, and choice of spatial scale is another factor that can impact the results. In this paper, different types of spatial scales (stand, theoretical landscape and real landscape) were used for assessing the time-dependent climate impact of bioenergy from short-rotation coppice willow and stumps harvested from conventional forests in Sweden. The result showed that the spatial scale has importance for the climate impact, especially for long-rotation forestry. However, the climate impact of both types of bioenergy systems was lower than for fossil coal over time, independently of spatial scale used. A landscape perspective was considered to be most relevant from a climate policy perspective.
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