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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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- Aad, G, et al.
(författare)
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- 2015
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swepub:Mat__t
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- Wang, Ning, et al.
(författare)
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Boride-derived oxygen-evolution catalysts
- 2021
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Metal borides/borates have been considered promising as oxygen evolution reaction catalysts; however, to date, there is a dearth of evidence of long-term stability at practical current densities. Here we report a phase composition modulation approach to fabricate effective borides/borates-based catalysts. We find that metal borides in-situ formed metal borates are responsible for their high activity. This knowledge prompts us to synthesize NiFe-Boride, and to use it as a templating precursor to form an active NiFe-Borate catalyst. This boride-derived oxide catalyzes oxygen evolution with an overpotential of 167 mV at 10 mA/cm2 in 1 M KOH electrolyte and requires a record-low overpotential of 460 mV to maintain water splitting performance for over 400 h at current density of 1 A/cm2. We couple the catalyst with CO reduction in an alkaline membrane electrode assembly electrolyser, reporting stable C2H4 electrosynthesis at current density 200 mA/cm2 for over 80 h.
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- Zhao, Meng, et al.
(författare)
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Three-dimensional cross-linked sugarcane bagasse carbon material: A substitute for graphene with excellent performance in capacitive deionization and highly efficient Cu2+removal
- 2024
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Ingår i: Colloids and Surfaces A. - : ELSEVIER. - 0927-7757 .- 1873-4359. ; 684
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Tidskriftsartikel (refereegranskat)abstract
- Capacitive deionization (CDI) is a high-performance, low-energy consumption, and environmentally friendly water treatment technology with a broad application prospect in heavy metal removal. Selecting electrode materials with high capacitance and low resistance is essential for improving CDI's desalting efficiency. This article discusses the utilization of sugarcane bagasse (C-N-X) and the production procedures of CDI materials. The unique 3D cross-linked structure of C-N-X provides excellent mass transfer properties and significant advantages in capacitance and conductivity. The results of X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared Spectrometer (FTIR) show that bagasse biochar with graphene-like structure and abundant functional groups provides active sites for Cu2+ removal. In this paper, C-N-X is first used as CDI electrode material to remove Cu2+. Electrochemical tests show that the specific capacitance of C-N-X is still stable at about 47 F g ? 1, and the removal capacity of Cu2+ (25 mg L-1) reaches 66.79 mg g-1 within 4 h after 700 cycles. The experimental results and DFT calculations confirm the adsorption selectivity of C -N-700 for Cu2+.
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