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Träfflista för sökning "WFRF:(Svedendahl M) "

Search: WFRF:(Svedendahl M)

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1.
  • Engelmark Cassimjee, Karim, et al. (author)
  • A general protein purification and immobilization method on controlled porosity glass : biocatalytic applications
  • 2014
  • In: Chemical Communications. - : Royal Society of Chemistry. - 1359-7345 .- 1364-548X. ; 50:65, s. 9134-9137
  • Journal article (peer-reviewed)abstract
    • A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
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2.
  • Chen, Si, 1985, et al. (author)
  • Ultrahigh sensitivity made simple : nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics.
  • 2009
  • In: Nanotechnology. - : Institute of Physics (IOP). - 0957-4484 .- 1361-6528. ; 20:43
  • Journal article (peer-reviewed)abstract
    • We present a simple and robust scheme for biosensing with an ultralow limit-of-detection down to several pg cm(-2) (or several tens of attomoles cm(-2)) based on optical label-free biodetection with localized surface plasmon resonances. The scheme utilizes cost-effective optical components and comprises a white light source, a properly functionalized sensor surface enclosed in a simple fluidics chip, and a spectral analyzer. The sensor surface is produced by a bottom-up nanofabrication technique with hole mask colloidal lithography. Despite its simplicity, the method is able to reliably detect protein-protein binding events at low picomolar and femtomolar concentrations, which is exemplified by the label-free detection of the extracellular adherence protein (EAP) found on the outer surface of the bacterium Staphylococcus aureus and of prostate-specific antigen (PSA), which is believed to be a prostate cancer marker. These experiments pave the way towards an ultra-sensitive yet compact biodetection platform for point-of-care diagnostics applications.
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