| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Liang, Xinyue, et al.
(författare)
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AVIATOR: fAst Visual Perception and Analytics for Drone-Based Traffic Operations
- 2023
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Ingår i: 2023 IEEE 26th International Conference on Intelligent Transportation Systems, ITSC 2023. - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 2959-2964
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Konferensbidrag (refereegranskat)abstract
- Drone-based system is an emerging technology for advanced applications in Intelligent Transport Systems (ITS). This paper presents our latest developments of a visual perception and analysis system, called AVIATOR, for drone-based road traffic management. The system advances from the previous SeeFar system in several aspects. For visual perception, deep-learning based computer vision models still play the central role but the current system development focuses on fast and efficient detection and tracking performance during real-time image processing. To achieve that, YOLOv7 and ByteTrack models have replaced the previous perception modules to gain better computational performance. Meanwhile, a lane-based traffic steam detection module is added for recognizing detailed traffic flow per lane, enabling more detailed estimation of traffic flow patterns. The traffic analytics module has been modified to estimate traffic states using lane-based data collection. This includes detailed lane-based traffic flow counting as well as traffic density estimation according to vehicle arrival patterns per lane.
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| 3. |
- Ma, Xiaoliang, Docent, et al.
(författare)
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METRIC : Toward a Drone-based Cyber-Physical Traffic Management System
- 2022
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Ingår i: Conference Proceedings - IEEE International Conference on Systems, Man and Cybernetics. - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 3324-3329
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Konferensbidrag (refereegranskat)abstract
- Drone-based system has a big potential to be applied for traffic monitoring and other advanced applications in Intelligent Transport Systems (ITS). This paper introduces our latest efforts of digitalising road traffic by various types of sensing systems, among which visual detection by drones provides a promising technical solution. A platform, called METRIC, is under recent development to carry out real-time traffic measurement and prediction using drone-based data collection. The current system is designed as a cyber-physical system (CPS) with essential functions aiming for visual traffic detection and analysis, real-time traffic estimation and prediction as well as decision supports based on simulation. In addition to the computer vision functions developed in the earlier stage, this paper also presents the CPS system architecture and the current implementation of the drone front-end system and a simulation-based system being used for further drone operations.
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