| 1. |
- Dong, Mei, et al.
(author)
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Cold Exposure Promotes Atherosclerotic Plaque Growth and Instability via UCP1-Dependent Lipolysis
- 2013
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In: Cell Metabolism. - : Elsevier (Cell Press). - 1550-4131 .- 1932-7420. ; 18:1, s. 118-129
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Journal article (peer-reviewed)abstract
- Molecular mechanisms underlying the cold-associated high cardiovascular risk remain unknown. Here, we show that the cold-triggered food-intake-independent lipolysis significantly increased plasma levels of small low-density lipoprotein (LDL) remnants, leading to accelerated development of atherosclerotic lesions in mice. In two genetic mouse knockout models (apolipoprotein E-/- [ApoE(-/-)] and LDL receptor(-/-) [Ldlr(-/-)] mice), persistent cold exposure stimulated atherosclerotic plaque growth by increasing lipid deposition. Furthermore, marked increase of inflammatory cells and plaque-associated microvessels were detected in the cold-acclimated ApoE(-/-) and Ldlr(-/-) mice, leading to plaque instability. Deletion of uncoupling protein 1 (UCP1), a key mitochondrial protein involved in thermogenesis in brown adipose tissue (BAT), in the ApoE(-/-) strain completely protected mice from the cold-induced atherosclerotic lesions. Cold acclimation markedly reduced plasma levels of adiponectin, and systemic delivery of adiponectin protected ApoE(-/-) mice from plaque development. These findings provide mechanistic insights on low-temperature-associated cardiovascular risks.
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| 2. |
- Zhang, Fan, et al.
(author)
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Proliferative and Survival Effects of PUMA Promote Angiogenesis
- 2012
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In: Cell Reports. - : Elsevier (Cell Press). - 2211-1247. ; 2:5, s. 1272-1285
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Journal article (peer-reviewed)abstract
- The p53 upregulated modulator of apoptosis (PUMA) is known as an essential apoptosis inducer. Here, we report the seemingly paradoxical finding that PUMA is a proangiogenic factor critically required for the proliferation and survival of vascular and microglia cells. Strikingly, Puma deficiency by genetic deletion or small hairpin RNA knockdown inhibited developmental and pathological angiogenesis and reduced microglia numbers in vivo, whereas Puma gene delivery increased angiogenesis and cell survival. Mechanistically, we revealed that PUMA plays a critical role in regulating autophagy by modulating Erk activation and intracellular calcium level. Our findings revealed an unexpected function of PUMA in promoting angiogenesis and warrant more careful investigations into the therapeutic potential of PUMA in treating cancer and degenerative diseases.
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| 3. |
- Lee, Chunsik, et al.
(author)
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VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway
- 2023
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In: SIGNAL TRANSDUCTION AND TARGETED THERAPY. - : SPRINGERNATURE. - 2095-9907 .- 2059-3635. ; 8:1
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Journal article (peer-reviewed)abstract
- Although VEGF-B was discovered as a VEGF-A homolog a long time ago, the angiogenic effect of VEGF-B remains poorly understood with limited and diverse findings from different groups. Notwithstanding, drugs that inhibit VEGF-B together with other VEGF family members are being used to treat patients with various neovascular diseases. It is therefore critical to have a better understanding of the angiogenic effect of VEGF-B and the underlying mechanisms. Using comprehensive in vitro and in vivo methods and models, we reveal here for the first time an unexpected and surprising function of VEGF-B as an endogenous inhibitor of angiogenesis by inhibiting the FGF2/FGFR1 pathway when the latter is abundantly expressed. Mechanistically, we unveil that VEGF-B binds to FGFR1, induces FGFR1/VEGFR1 complex formation, and suppresses FGF2-induced Erk activation, and inhibits FGF2-driven angiogenesis and tumor growth. Our work uncovers a previously unrecognized novel function of VEGF-B in tethering the FGF2/FGFR1 pathway. Given the anti-angiogenic nature of VEGF-B under conditions of high FGF2/FGFR1 levels, caution is warranted when modulating VEGF-B activity to treat neovascular diseases.
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| 4. |
- Drottberger, Annie, et al.
(author)
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Urban farming with rooftop greenhouses : A systematic literature review
- 2023
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In: Renewable and Sustainable Energy Reviews. - 1364-0321 .- 1879-0690. ; 188
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Research review (peer-reviewed)abstract
- The environmental impacts of food systems will increase in tandem with rapid urban population growth, which calls for alternative solutions, such as urban agriculture, to reach the United Nations Sustainable Development Goals. Among several urban agriculture systems, rooftop farming and its subset, rooftop greenhouses, are promising technologies. They optimize land use, increase profitability for building owners, deliver good yields per unit area, increase water use efficiency, and reduce the energy use of both greenhouse and host buildings while mitigating the urban heat island effect. A systematic literature review of the rooftop greenhouse technology was carried out to examine the benefits and challenges associated with this technology. This review was based on 45 articles, covering themes such as the impact of rooftop greenhouse technology on yields, energy use, water use, environmental impacts, and life-cycle costs; some benefits identified are the symbiotic heat, water, and CO2 exchanges between the rooftop greenhouse and its host building, and the possibility of delivering year-round production. The additional investment, operational costs, limited availability of flat roofs, and various regulations were challenges to overcome. The relevance of symbiosis between rooftop greenhouses and buildings to enhancing sustainability, and meeting the SDGs was explored. This review also outlines that rooftop greenhouses are increasing in scale, system diversity, societal acceptance and popularity among commercial operations in large cities. The future of rooftop farming lies in customizing the right technology for selected building typologies globally, where food production is fully integrated into the urban landscape.
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| 5. |
- Eleonora Hedlund, Eva-Maria, et al.
(author)
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Tumor cell-derived placental growth factor sensitizes antiangiogenic and antitumor effects of anti-VEGF drugs
- 2013
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In: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 110:2, s. 654-659
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Journal article (peer-reviewed)abstract
- The role of placental growth factor (PlGF) in modulation of tumor angiogenesis and tumor growth remains an enigma. Furthermore, anti-PlGF therapy in tumor angiogenesis and tumor growth remains controversial in preclinical tumor models. Here we show that in both human and mouse tumors, PlGF induced the formation of dilated and normalized vascular networks that were hypersensitive to anti-VEGF and anti-VEGFR-2 therapy, leading to dormancy of a substantial number of avascular tumors. Loss-of-function using plgf shRNA in a human choriocarcinoma significantly accelerated tumor growth rates and acquired resistance to anti-VEGF drugs, whereas gain-of-function of PlGF in a mouse tumor increased anti-VEGF sensitivity. Further, we show that VEGFR-2 and VEGFR-1 blocking antibodies displayed opposing effects on tumor angiogenesis. VEGFR-1 blockade and genetic deletion of the tyrosine kinase domain of VEGFR-1 resulted in enhanced tumor angiogenesis. These findings demonstrate that tumor-derived PlGF negatively modulates tumor angiogenesis and tumor growth and may potentially serve as a predictive marker of anti-VEGF cancer therapy.
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| 6. |
- Wells, Matthew P., et al.
(author)
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Pathway to high performance, low temperature thin-film solid oxide cells grown on porous anodised aluminium oxide
- 2024
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In: Nano Energy. - : ELSEVIER. - 2211-2855 .- 2211-3282. ; 119
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Journal article (peer-reviewed)abstract
- Reversible solid oxide cells (rSOCs) present a promising solution to future energy challenges through the efficient conversion between electrical and chemical energy. To date, the benefits of rSOC technology have been off-limits to portable power and electrolysis applications due to the excessive polarisation resistance of the oxygen electrode at low temperatures, characterised by high area specific resistance (ASR) values below 500 degrees C. In this work we demonstrate growth of symmetric and complete rSOC structures based on state-of-the-art vertically aligned nanocomposite (VAN) films grown by pulsed laser deposition (PLD) on porous Pt-coated anodised aluminium oxide (AAO) substrates. The symmetric rSOC structures give the first demonstration of an rSOC oxygen electrode with ASR below 0.1 ohm cm2 at temperatures less than 450 degrees C. This is achieved through oxygen vacancy tuning by annealing, as confirmed by Time-of-Flight Elastic Recoil Detection Analysis (ToF-ERDA) and Rutherford Backscattering Spectrometry (RBS) measurements. Thus, the present work describes a promising route towards future high-performance rSOC devices for portable power applications.
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