| 1. |
|
|
| 2. |
- Ahlberg, Mats Steinholtz, 1979-
(author)
-
Surveillance and follow-up of early prostate cancer
- 2024
-
Doctoral thesis (other academic/artistic)abstract
- Active surveillance (AS) for prostate cancer was introduced to address overtreatment resulting from prostate-specific antigen (PSA) testing. Despite advancements such as Magnetic Resonance Imaging (MRI) and targeted biopsies, PSA remains crucial in prostate cancer diagnostics, leading to ongoing challenges of overdiagnosis and overtreatment. This thesis aimed to investigate different aspects of AS and follow-up of early prostate cancer and provide new insights to reduce overtreatment and enhance surveillance and follow-up. In Paper I, the rationale and methodology of a randomized controlled trial, the Prostate Cancer Active Surveillance Trigger trial/Scandinavian Prostate Cancer Group study no. 17 (PCASTt/SPCG17), were outlined. This trial's objective is to evaluate the safety of an AS protocol based on MRI and standardized triggers for repeat biopsies and transition to radical treatment. Patient recruitment is anticipated to conclude in 2024. Paper II investigated the risks of biochemical recurrence, metastatic disease, and prostate cancer-related death in patients following radical prostatectomy. The analysis was conditioned on time after radical prostatectomy without biochemical recurrence. For patients with favourable histopathology in prostatectomy specimens and no biochemical recurrence five years post-prostatectomy, the probability of developing metastatic disease or dying from prostate cancer within 20 years after surgery was very low. This suggests shorter follow-up for selected patients. Paper III compared outcomes of AS for men from different healthcare regions in Sweden with varying traditions of AS. Regions with lower uptake in AS demonstrated a higher probability of transitioning from AS to radical treatment, but no difference in AS failure. The results suggest overtreatment in regions with low uptake in AS. Paper IV explored the associations between potential triggers for transitioning from AS to radical treatment and the transition to treatment. We analysed how this association changed with the introduction of prostate MRI. We found an increasingly strong association between triggers, particularly histopathological progression, and transition. However, most treated men had not experienced histopathological progression. The introduction of MRI did not contribute much to the change. In conclusion, this thesis outlines an ongoing study on defined triggers for transitioning from AS to radical treatment, suggests shorter follow-up after radical prostatectomy for selected patients, reveals overtreatment in regions with low uptake in AS, and shows an increasing use of histopathological progression as a trigger for transition to radical treatment.
|
|
| 3. |
- Carlquist, Magnus, et al.
(author)
-
Physiological heterogeneities in microbial populations and implications for physical stress tolerance
- 2012
-
In: Microbial Cell Factories. - : Springer Science and Business Media LLC. - 1475-2859. ; 11
-
Journal article (peer-reviewed)abstract
- Background: Traditionally average values of the whole population are considered when analysing microbial cell cultivations. However, a typical microbial population in a bioreactor is heterogeneous in most phenotypes measurable at a single-cell level. There are indications that such heterogeneity may be unfavourable on the one hand (reduces yields and productivities), but also beneficial on the other hand (facilitates quick adaptation to new conditions - i.e. increases the robustness of the fermentation process). Understanding and control of microbial population heterogeneity is thus of major importance for improving microbial cell factory processes. Results: In this work, a dual reporter system was developed and applied to map growth and cell fitness heterogeneities within budding yeast populations during aerobic cultivation in well-mixed bioreactors. The reporter strain, which was based on the expression of green fluorescent protein (GFP) under the control of the ribosomal protein RPL22a promoter, made it possible to distinguish cell growth phases by the level of fluorescence intensity. Furthermore, by exploiting the strong correlation of intracellular GFP level and cell membrane integrity it was possible to distinguish subpopulations with high and low cell membrane robustness and hence ability to withstand freeze-thaw stress. A strong inverse correlation between growth and cell membrane robustness was observed, which further supports the hypothesis that cellular resources are limited and need to be distributed as a trade-off between two functions: growth and robustness. In addition, the trade-off was shown to vary within the population, and the occurrence of two distinct subpopulations shifting between these two antagonistic modes of cell operation could be distinguished. Conclusions: The reporter strain enabled mapping of population heterogeneities in growth and cell membrane robustness towards freeze-thaw stress at different phases of cell cultivation. The described reporter system is a valuable tool for understanding the effect of environmental conditions on population heterogeneity of microbial cells and thereby to understand cell responses during industrial process-like conditions. It may be applied to identify more robust subpopulations, and for developing novel strategies for strain improvement and process design for more effective bioprocessing.
|
|
| 4. |
|
|
| 5. |
- Fernandes, Rita Lencastre, et al.
(author)
-
Applying Mechanistic Models in Bioprocess Development
- 2013
-
In: Advances in Biochemical Engineering, Biotechnology. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 0724-6145. ; 132, s. 137-166
-
Journal article (peer-reviewed)abstract
- The available knowledge on the mechanisms of a bioprocess system is central to process analytical technology. In this respect, mechanistic modeling has gained renewed attention, since a mechanistic model can provide an excellent summary of available process knowledge. Such a model therefore incorporates process-relevant input (critical process variables)-output (product concentration and product quality attributes) relations. The model therefore has great value in planning experiments, or in determining which critical process variables need to be monitored and controlled tightly. Mechanistic models should be combined with proper model analysis tools, such as uncertainty and sensitivity analysis. When assuming distributed inputs, the resulting uncertainty in the model outputs can be decomposed using sensitivity analysis to determine which input parameters are responsible for the major part of the output uncertainty. Such information can be used as guidance for experimental work; i.e., only parameters with a significant influence on model outputs need to be determined experimentally. The use of mechanistic models and model analysis tools is demonstrated in this chapter. As a practical case study, experimental data from Saccharomyces cerevisiae fermentations are used. The data are described with the well-known model of Sonnleitner and Kappeli (Biotechnol Bioeng 28: 927-937, 1986) and the model is analyzed further. The methods used are generic, and can be transferred easily to other, more complex case studies as well.
|
|
| 6. |
- Fernandes, Rita Lencastre, et al.
(author)
-
Cell mass and cell cycle dynamics of an asynchronous budding yeast population: Experimental observations, flow cytometry data analysis, and multi-scale modeling
- 2013
-
In: Biotechnology and Bioengineering. - : Wiley. - 1097-0290 .- 0006-3592. ; 110:3, s. 812-826
-
Journal article (peer-reviewed)abstract
- Despite traditionally regarded as identical, cells in a microbial cultivation present a distribution of phenotypic traits, forming a heterogeneous cell population. Moreover, the degree of heterogeneity is notably enhanced by changes in micro-environmental conditions. A major development in experimental single-cell studies has taken place in the last decades. It has however not been fully accompanied by similar contributions within data analysis and mathematical modeling. Indeed, literature reporting, for example, quantitative analyses of experimental single-cell observations and validation of model predictions for cell property distributions against experimental data is scarce. This study focuses on the experimental and mathematical description of the dynamics of cell size and cell cycle position distributions, of a population of Saccharomyces cerevisiae, in response to the substrate consumption observed during batch cultivation. The good agreement between the proposed multi-scale model (a population balance model [PBM] coupled to an unstructured model) and experimental data (both the overall physiology and cell size and cell cycle distributions) indicates that a mechanistic model is a suitable tool for describing the microbial population dynamics in a bioreactor. This study therefore contributes towards the understanding of the development of heterogeneous populations during microbial cultivations. More generally, it consists of a step towards a paradigm change in the study and description of cell cultivations, where average cell behaviors observed experimentally now are interpreted as a potential joint result of various co-existing single-cell behaviors, rather than a unique response common to all cells in the cultivation. Biotechnol. Bioeng. 2013; 110: 812826. (c) 2012 Wiley Periodicals, Inc.
|
|
| 7. |
- Heins, Anna Lena, et al.
(author)
-
Quantitative flow cytometry to understand population heterogeneity in response to changes in substrate availability in escherichia coli and saccharomyces cerevisiae chemostats
- 2019
-
In: Frontiers in Bioengineering and Biotechnology. - : Frontiers Media SA. - 2296-4185. ; 7:AUG
-
Journal article (peer-reviewed)abstract
- Microbial cells in bioprocesses are usually described with averaged parameters. But in fact, single cells within populations vary greatly in characteristics such as stress resistance, especially in response to carbon source gradients. Our aim was to introduce tools to quantify population heterogeneity in bioprocesses using a combination of reporter strains, flow cytometry, and easily comprehensible parameters. We calculated mean, mode, peak width, and coefficient of variance to describe distribution characteristics and temporal shifts in fluorescence intensity. The skewness and the slope of cumulative distribution function plots illustrated differences in distribution shape. These parameters are person-independent and precise. We demonstrated this by quantifying growth-related population heterogeneity of Saccharomyces cerevisiae and Escherichia coli reporter strains in steady-state of aerobic glucose-limited chemostat cultures at different dilution rates and in response to glucose pulses. Generally, slow-growing cells showed stronger responses to glucose excess than fast-growing cells. Cell robustness, measured as membrane integrity after exposure to freeze-thaw treatment, of fast-growing cells was strongly affected in subpopulations of low membrane robustness. Glucose pulses protected subpopulations of fast-growing but not slower-growing yeast cells against membrane damage. Our parameters could successfully describe population heterogeneity, thereby revealing physiological characteristics that might have been overlooked during traditional averaged analysis.
|
|
| 8. |
|
|
| 9. |
- Alvekrans, Anna-Lena, et al.
(author)
-
From Knowledge to Decision - A Case Study of Sales and Operations Planning in Health Care
- 2016
-
In: Production planning & control. - : Informa UK Limited. - 0953-7287 .- 1366-5871. ; 27:12, s. 1019-1026
-
Journal article (peer-reviewed)abstract
- One of the most important challenges in health care management is balancing resources with fluctuating and uncertain demand. This study examines whether introducing a standardised process for Sales and Operations planning at a large university hospital improved specialty department managers’ knowledge of planning conditions and if so, whether decisions were made based on this knowledge. Using responses from a survey of 30 specialty department managers at Sahlgrenska University Hospital in Sweden, the analyses indicate that operational knowledge strengthens insights about the needs for strategic decisions, and vice versa. Moreover, knowledge is a necessary, but not sufficient, condition for making decisions at the department level. An overall planning and decision structure is required and should be implemented first at the hospital level to effectively allocate resources.
|
|
| 10. |
|
|
| 11. |
- Arndt, D. S., et al.
(author)
-
State of the Climate in 2016
- 2017
-
In: Bulletin of The American Meteorological Society - (BAMS). - 0003-0007 .- 1520-0477. ; 98:8, s. S1-S280
-
Journal article (peer-reviewed)abstract
- In 2016, the dominant greenhouse gases released into Earth's atmosphere-carbon dioxide, methane, and nitrous oxide-continued to increase and reach new record highs. The 3.5 +/- 0.1 ppm rise in global annual mean carbon dioxide from 2015 to 2016 was the largest annual increase observed in the 58-year measurement record. The annual global average carbon dioxide concentration at Earth's surface surpassed 400 ppm (402.9 +/- 0.1 ppm) for the first time in the modern atmospheric measurement record and in ice core records dating back as far as 800000 years. One of the strongest El Nino events since at least 1950 dissipated in spring, and a weak La Nina evolved later in the year. Owing at least in part to the combination of El Nino conditions early in the year and a long-term upward trend, Earth's surface observed record warmth for a third consecutive year, albeit by a much slimmer margin than by which that record was set in 2015. Above Earth's surface, the annual lower troposphere temperature was record high according to all datasets analyzed, while the lower stratospheric temperature was record low according to most of the in situ and satellite datasets. Several countries, including Mexico and India, reported record high annual temperatures while many others observed near-record highs. A week-long heat wave at the end of April over the northern and eastern Indian peninsula, with temperatures surpassing 44 degrees C, contributed to a water crisis for 330 million people and to 300 fatalities. In the Arctic the 2016 land surface temperature was 2.0 degrees C above the 1981-2010 average, breaking the previous record of 2007, 2011, and 2015 by 0.8 degrees C, representing a 3.5 degrees C increase since the record began in 1900. The increasing temperatures have led to decreasing Arctic sea ice extent and thickness. On 24 March, the sea ice extent at the end of the growth season saw its lowest maximum in the 37-year satellite record, tying with 2015 at 7.2% below the 1981-2010 average. The September 2016 Arctic sea ice minimum extent tied with 2007 for the second lowest value on record, 33% lower than the 1981-2010 average. Arctic sea ice cover remains relatively young and thin, making it vulnerable to continued extensive melt. The mass of the Greenland Ice Sheet, which has the capacity to contribute similar to 7 m to sea level rise, reached a record low value. The onset of its surface melt was the second earliest, after 2012, in the 37-year satellite record. Sea surface temperature was record high at the global scale, surpassing the previous record of 2015 by about 0.01 degrees C. The global sea surface temperature trend for the 21st century-to-date of +0.162 degrees C decade(-1) is much higher than the longer term 1950-2016 trend of +0.100 degrees C decade(-1). Global annual mean sea level also reached a new record high, marking the sixth consecutive year of increase. Global annual ocean heat content saw a slight drop compared to the record high in 2015. Alpine glacier retreat continued around the globe, and preliminary data indicate that 2016 is the 37th consecutive year of negative annual mass balance. Across the Northern Hemisphere, snow cover for each month from February to June was among its four least extensive in the 47-year satellite record. Continuing a pattern below the surface, record high temperatures at 20-m depth were measured at all permafrost observatories on the North Slope of Alaska and at the Canadian observatory on northernmost Ellesmere Island. In the Antarctic, record low monthly surface pressures were broken at many stations, with the southern annular mode setting record high index values in March and June. Monthly high surface pressure records for August and November were set at several stations. During this period, record low daily and monthly sea ice extents were observed, with the November mean sea ice extent more than 5 standard deviations below the 1981-2010 average. These record low sea ice values contrast sharply with the record high values observed during 2012-14. Over the region, springtime Antarctic stratospheric ozone depletion was less severe relative to the 1991-2006 average, but ozone levels were still low compared to pre-1990 levels. Closer to the equator, 93 named tropical storms were observed during 2016, above the 1981-2010 average of 82, but fewer than the 101 storms recorded in 2015. Three basins-the North Atlantic, and eastern and western North Pacific-experienced above-normal activity in 2016. The Australian basin recorded its least active season since the beginning of the satellite era in 1970. Overall, four tropical cyclones reached the Saffir-Simpson category 5 intensity level. The strong El Nino at the beginning of the year that transitioned to a weak La Nina contributed to enhanced precipitation variability around the world. Wet conditions were observed throughout the year across southern South America, causing repeated heavy flooding in Argentina, Paraguay, and Uruguay. Wetter-than-usual conditions were also observed for eastern Europe and central Asia, alleviating the drought conditions of 2014 and 2015 in southern Russia. In the United States, California had its first wetter-than-average year since 2012, after being plagued by drought for several years. Even so, the area covered by drought in 2016 at the global scale was among the largest in the post-1950 record. For each month, at least 12% of land surfaces experienced severe drought conditions or worse, the longest such stretch in the record. In northeastern Brazil, drought conditions were observed for the fifth consecutive year, making this the longest drought on record in the region. Dry conditions were also observed in western Bolivia and Peru; it was Bolivia's worst drought in the past 25 years. In May, with abnormally warm and dry conditions already prevailing over western Canada for about a year, the human-induced Fort McMurray wildfire burned nearly 590000 hectares and became the costliest disaster in Canadian history, with $3 billion (U.S. dollars) in insured losses.
|
|
| 12. |
|
|
| 13. |
- Artman, Henrik, et al.
(author)
-
Acquisition of usable IT : Acquisition projects to reflect on
- 2009
-
Reports (other academic/artistic)abstract
- By examining how several organizations have gone through the process of procuring IT systems, we have seen that there is a great need for procurer organizations themselves to understand their role in systems development. What is their responsibility for the outcome of the acquisition process? What is their responsibility for the outcome of the system-in-use? Can they actually take responsibility for the usability of systems? This collection of papers is meant to be a starting point for procurer organizations to reflect on that responsibility, as well as on how they manage the acquisition process. The papers are informed by academic research and grounded in scientific studies, but they are also to be taken as practical efforts to describe the process. We hope they will nurture reflection, and encourage those who are taking a stand to make IT systems usable. Our assumption is that the sooner an organization comes to terms with how the future system will actually be used, the sooner it will be profitable or beneficial.
|
|
| 14. |
|
|
| 15. |
- Battistoni, Giuseppe, et al.
(author)
-
Applications of FLUKA Monte Carlo code for nuclear and accelerator physics
- 2011
-
In: Nuclear Instruments and Methods in Physics Research Section B. - : Elsevier BV. - 0168-583X .- 1872-9584. ; 269:24, s. 2850-2856
-
Journal article (peer-reviewed)abstract
- FLUKA is a general purpose Monte Carlo code capable of handling all radiation components from thermal energies (for neutrons) or 1 keV (for all other particles) to cosmic ray energies and can be applied in many different fields. Presently the code is maintained on Linux. The validity of the physical models implemented in FLUKA has been benchmarked against a variety of experimental data over a wide energy range, from accelerator data to cosmic ray showers in the Earth atmosphere. FLUKA is widely used for studies related both to basic research and to applications in particle accelerators, radiation protection and dosimetry, including the specific issue of radiation damage in space missions, radiobiology (including radiotherapy) and cosmic ray calculations.After a short description of the main features that make FLUKA valuable for these topics, the present paper summarizes some of the recent applications of the FLUKA Monte Carlo code in the nuclear as well high energy physics. In particular it addresses such topics as accelerator related applications.
|
|
| 16. |
- Battistoni, Giuseppe, et al.
(author)
-
FLUKA Capabilities and CERN Applications for the Study of Radiation Damage to Electronics at High-Energy Hadron Accelerators
- 2011
-
Conference paper (peer-reviewed)abstract
- The assessment of radiation damage to electronics is a complex process and requires a detailed description of the full particle energy spectra, as well as a clear characterization of the quantities used to predict radiation damage. FLUKA, a multi-purpose particle interaction and transport code, is capable of calculating proton-proton and heavy ion collisions at LHC energies and beyond. It correctly describes the entire hadronic and electromagnetic particle cascade initiated by secondary particles from TeV energies down to thermal neutrons, and provides direct scoring capabilities essential to estimate in detail the possible risk of radiation damage to electronics. This paper presents the FLUKA capabilities for applications related to radiation damage to electronics, providing benchmarking examples and showing the practical applications of FLUKA at CERN facilities such as CNGS and LHC. Related applications range from the study of device effects, the detailed characterization of the radiation field and radiation monitor calibration, to the input requirements for important mitigation studies including shielding, relocation or other options.
|
|
| 17. |
- Battistoni, Giuseppe, et al.
(author)
-
The Application of the Monte Carlo Code FLUKA in Radiation Protection Studies for the Large Hadron Collider
- 2011
-
Conference paper (peer-reviewed)abstract
- The multi-purpose particle interaction and transport code FLUKA is integral part of all radiation protection studies for the design and operation of the Large Hadron Collider (LHC) at CERN. It is one of the very few codes available for this type of calculations which is capable to calculate in one and the same simulation proton-proton and heavy ion collisions at LHC energies as well as the entire hadronic and electromagnetic particle cascade initiated by secondary particles in detectors and beam-line components from TeV energies down to energies of thermal neutrons. The present paper reviews these capabilities of FLUKA in sketching the relevant physics models along with examples ofradiation protection studies for the LHC such as shielding studies for underground areas occupied by personnel during LHC operation and the simulation of induced radioactivity around beam loss points. Integral part of the FLUKA development is a careful benchmarking of specific models as well as the code performance in actual, complex applications which is demonstrated with examples of studies relevant to radiation protection at the LHC.
|
|
| 18. |
- Bratt, Ola, 1963, et al.
(author)
-
Population-based Organised Prostate Cancer Testing: Results from the First Invitation of 50-year-old Men
- 2024
-
In: European Urology. - 0302-2838 .- 1873-7560. ; 85:3, s. 207-214
-
Journal article (peer-reviewed)abstract
- Background: The European Union recently recommended evaluation of the feasibility of organised prostate cancer screening. In Sweden, regional population-based organised prostate cancer testing (OPT) programmes were introduced in 2020. Objective: To describe initial participation rates and diagnostic outcomes. Design, setting, and participants: The three most populated Swedish regions invited all men aged 50 yr to OPT by a letter in 2020–2022. Men with prostate-specific antigen (PSA) ≥3 ng/ml were referred for prostate magnetic resonance imaging (MRI). PSA assays differed across regions. Men with Prostate Imaging Reporting and Data System (PI-RADS) 1–3 and PSA density ≥0.15 ng/ml/cm3 or PI-RADS 4–5 were referred for a biopsy. Data were obtained from the Swedish Register for Organised Prostate Cancer Testing. Outcome measurements and statistical analysis: Overall and regional participation rates, PSA distributions, PI-RADS score distributions, cancer detection, and treatment were evaluated. Results and limitations: A total of 23 855 (35%) of 68 060 invited men participated; 696 (2.9%) had PSA ≥3 ng/ml, and of them, 306 (44%) had a biopsy indication and 221 (32%) had a biopsy. On biopsy, 93 (42%) had Gleason grade group ≥2 (0.39% of PSA-tested men) and 44 (20%) Gleason grade group 1 cancer. Most men with cancer had treatment with curative intent (70%) or were under active surveillance (28%). Across regions, proportions of men with PSA ≥3 ng/ml ranged from 2.3% to 4.0%, and those with PI-RADS score 4–5 ranged from 12% to 21%. A limitation is that results are applicable only to first testing of men in their early 50s. Conclusions: The OPT programmes are feasible with good compliance to the diagnostic pathway. The use of MRI and PSA density avoided a biopsy for over half of the men with PSA ≥3 ng/ml. Inter-regional differences in diagnostic outcomes show a need for standardisation of the diagnostic pathway's components. Patient summary: We report the diagnostic outcomes of inviting 68 000 50-yr-old men to organised prostate cancer testing.
|
|
| 19. |
- Carlsson, Stefan, et al.
(author)
-
Salvage radiotherapy after radical prostatectomy: functional outcomes in the LAPPRO trial after 8-year follow-up.
- 2023
-
In: Scandinavian journal of urology. - 2168-1813. ; 58, s. 11-19
-
Journal article (peer-reviewed)abstract
- Radical prostatectomy reduces mortality among patients with localized prostate cancer, however up to 35% of patients will experience biochemical recurrence, often treated with salvage radiotherapy. The objective of the study was to investigate long-term effects of salvage radiotherapy.A prospective, controlled, non-randomized trial at 14 Swedish center's including 4,003 patients scheduled for radical prostatectomy 2008-2011. A target trial emulation approach was used to identify eligible patients that was treated with salvage radiotherapy. The control group received no salvage radiotherapy. Outcomes were assessed by patient questionnaires on ordinal scales and statistical group comparisons were made using ordered logit regression with adjustment for baseline outcome and confounding factors. The primary endpoints were bowel, urinary and sexual function and bothering due to dysfunction at 8 years.Eleven percent (330/3,139) of the analyzed study population received salvage radiotherapy. Fecal leakage, leakage of mucus and hematochezia were more common after receiving salvage radiotherapy compared with the control group; 4.5% versus 2.6% odds ratio (95% confidence interval [CI]): (1.90 [1.38; 2.62]), 6.8% versus 1.5% 4.14 (2.98; 5.76) and 8.6% versus 1.2% 4.14 (2.98; 5.76), respectively. Urinary incontinence, erectile dysfunction and hematuria were more common after receiving salvage radiotherapy, 34% versus 23% 2.23 (2.65; 3.00), 65% versus 57% 1.65 (1.18; 2.29) and 16% versus 1.6% 11.17 (5.68; 21.99), respectively.Salvage radiotherapy was associated with increased risk for fecal leakage, hematochezia, urinary incontinence and hematuria. Our results emphasize the importance of selecting patients for salvage radiotherapy to avoid overtreatment and to give high quality pre-treatment information to ensure patients' preparedness for late side-effects.
|
|
| 20. |
|
|
| 21. |
- Dominguez Del Angel, Victoria, et al.
(author)
-
Ten steps to get started in Genome Assembly and Annotation.
- 2018
-
In: F1000Research. - : F1000 Research Ltd. - 2046-1402. ; 7
-
Journal article (peer-reviewed)abstract
- As a part of the ELIXIR-EXCELERATE efforts in capacity building, we present here 10 steps to facilitate researchers getting started in genome assembly and genome annotation. The guidelines given are broadly applicable, intended to be stable over time, and cover all aspects from start to finish of a general assembly and annotation project. Intrinsic properties of genomes are discussed, as is the importance of using high quality DNA. Different sequencing technologies and generally applicable workflows for genome assembly are also detailed. We cover structural and functional annotation and encourage readers to also annotate transposable elements, something that is often omitted from annotation workflows. The importance of data management is stressed, and we give advice on where to submit data and how to make your results Findable, Accessible, Interoperable, and Reusable (FAIR).
|
|
| 22. |
- Eliasson Lantz, Anna, et al.
(author)
-
On-line monitoring of fermentation processes in lignocellulose-to-bioalcohol production.
- 2010
-
In: Bioalcohol production: Biochemical conversion of lignocellulosic biomass. - : Elsevier. - 9781845695101 ; , s. 315-339
-
Book chapter (other academic/artistic)abstract
- Online monitoring of bioethanol production is challenging due to the complex, viscous sample matrix, also containing solids. Suitable targets for online measurement are identified and potential monitoring techniques in addition to standard physicochemical measurements are reviewed. Advantages and limitations of chromatographic techniques, spectroscopic methods and software sensors are discussed in detail. In conclusion, bioethanol production processes should certainly benefit from online measurement of key process variables, especially if measurements are incorporated in process control loops. However, convincing case studies are lacking, and therefore development of suitable sampling methods and online measurement techniques should first be prioritized.
|
|
| 23. |
- Erestam, Sofia, et al.
(author)
-
Associations between intraoperative factors and surgeons' self-assessed operative satisfaction.
- 2020
-
In: Surgical endoscopy. - : Springer Science and Business Media LLC. - 1432-2218 .- 0930-2794. ; 34:1, s. 61-68
-
Journal article (peer-reviewed)abstract
- Little is known concerning what may influence surgeon satisfaction with a surgical procedure and its associations with intraoperative factors. The objective was to explore the relationships between surgeons' self-assessed satisfaction with performed radical prostatectomies and intraoperative factors such as technical difficulties and intraoperative complications as reported by the surgeon subsequent to the operation.We utilized prospectively collected data from the controlled LAPPRO trial where 4003 patients with prostate cancer underwent open (ORP) or robot-assisted laparoscopic (RALP) radical prostatectomy. Patients were included from fourteen centers in Sweden during 2008-2011. Surgeon satisfaction was assessed by questionnaires at the end of each operation. Intraoperative factors included time for the surgical procedure as well as difficulties and complications in various steps of the operation. To model surgeon satisfaction, a mixed effect logistic regression was used. Results were presented as odds ratios (OR) with 95% confidence intervals (CI).The surgeons were satisfied in 2905 (81%) and dissatisfied in 702 (19%) of the surgical procedures. Surgeon satisfaction was not statistically associated with type of surgical technique (ORP vs. RALP) (OR 1.36, CI 0.76; 2.43). Intraoperative factors such as technical difficulties or complications, for example, suturing of the anastomosis was negatively associated with surgeon satisfaction (OR 0.24, CI 0.19; 0.30).Our data indicate that technical difficulties and/or intraoperative complications were associated with a surgeon's level of satisfaction with an operation.
|
|
| 24. |
- Haglind, Therése
(author)
-
Digitalisering av gymnasieskolans verksamhet : tillgång och användning
- 2015
-
Licentiate thesis (other academic/artistic)abstract
- Skolan är en del av samhället och den digitala utvecklingen ger upphov till både möjligheter och utmaningar för skolans verksamhet. Denna licentiatuppsats handlar om digitaliseringen av svensk gymnasieskola och tar sin utgångspunkt i en verksamhet där tillgången till digitala verktyg på flera sätt är tillgodosedd. Syftet med detta arbete att undersöka användningen av digitala verktyg för skolarbete, samt förutsättningar för denna användning, i en miljö med god tillgång till digitala teknologiska resurser och en utbyggd infrastruktur. Tre forskningsfrågor är i fokus: (1) Hur ser användningen av skoldatorer och mobiltelefoner ut för skolarbete, både inom och utanför skolan? (2) Vad är elevernas och lärarnas uppfattning om digitala verktyg och deras användbarhet för pedagogiska syften? och (3) Vilka funktioner har skolans lärplattform i lärares gemensamma lektionsplanering? Forskningen har bedrivits nära praktiken och datainsamlingen har genomförts på två 1:1 gymnasieskolor i en medelstor stad i Sverige. Elevenkäter, enskilda lärarintervjuer, grupputvärdering med lärare och loggning av lärares aktivitet på LMS:et har genomförts under åren 2013-2015. Arbetet utgår ifrån ett sociokulturellt perspektiv och grundtankar är att lärandet är socialt och sker i interaktion med andra och omvärlden (Sutherland et al., 2009; Säljö, 2014). Delstudie 1 handlar om elevers användning av skoldatorn och den egna privata mobiltelefonen (Haglind, 2014c). Texten baseras på enkätundersökningar och fokusgruppintervjuer med elever genomförda på två 1:1 gymnasieskolor 2013. Delstudie 2 undersöker elevers uppfattningar om mobiltelefonen som ett verktyg för skolarbete. Texten baseras på en enkätundersökning genomförd 2014 (Ott, Haglind & Lindström, 2014). Delstudie 3 undersöker relationen mellan elevers tillgång och användning av digitala verktyg för skolarbete (Haglind, Godhe & Lindström, 2015) och delstudie 4 fokuserar på användningen av ett LMS under ett kollegialt samarbete samt hur detta samarbete har uppfattats (Haglind, 2015). Delstudie 3 bygger på material från fokusgruppintervjuer, två enkätundersökningar och lärarintervjuer. Delstudie 4 använder material från lärarintervjuer, muntlig grupputvärdering med lärare och loggad LMS-aktivitet. Resultaten i delstudierna visar att digitaliseringen av gymnasieskolan är en komplex fråga där flera faktorer spelar roll. Trots god tillgång till digitala verktyg i skolmiljön så är användningen relativt låg och det finns en skillnad mellan elever ifråga om både användning av och uppfattning om digitala verktyg i skolan. Vidare indikerar resultaten att eleverna på de högskoleförberedande programmen har en annan uppfattning om digitala verktyg som redskap för skolarbete än elever på yrkesförberedande program samt att elever därmed ges olika förutsättningar att bemästra IKT för att lära baserat på programinriktning. Resultaten visar att eleverna i hög uträckning tar med sig sina privata mobiltelefoner till skolan. En del elever använder dessa för skolarbete både i skolan och hemma, men majoriteten av eleverna gör det inte utan identifierar både hinder och begränsningar med att använda mobiltelefonen för skolarbete. Dessa resultat tyder på att mobiltelefonen inte utmanar de etablerade verktygen för skolarbete eftersom den inte i någon större utsträckning används för skolarbete. Men det finns elever som använder mobiltelefonen och datorn för att utföra olika typer av uppgifter. De använder till exempel mobiltelefonen för att översätta ord och kommunicera med sms och datorn för att exempelvis skriva texter och anteckna (Ott, Haglind & Lindström, 2014). Dessa resultat indikerar att en del elever använder de olika digitala verktygen för olika ändamål och att de på eget initiativ kan välja bland de tillgängliga digitala verktygen. Delstudie 4 visar att LMS får olika funktioner i lärares gemensamma undervisningsplanering. Förutom att vara en plats att dela och förvara material så visar studien att LMS skapar lärtillfällen för lärarna när de på egen hand ”bläddrar” igenom materialet för att se vilket material de kan använda sig av; det blir det ett tillfälle för reflektion över både sin egen och andras undervisning (Haglind, 2015). Generellt sett är tillgången till digitala verktyg hög i svensk gymnasieskola, men resultaten visar att det finns ett glapp mellan tillgång och användning. Det räcker därmed inte att undersöka tillgång utan andra faktorer såsom elever och lärares uppfattningar om digitala verktyg är viktiga att undersöka och förhålla sig till. Detta arbete visar också vikten av det kollegiala samarbetet och en central fråga här är vilka stödstrukturer som blir viktiga i den digitaliserade skolan. Avslutningsvis så betonas här att det behövs bedrivas vidare forskning om gymnasieskolans digitalisering, med fördel genom studier där den beprövade erfarenheten ges möjlighet att grundas i vetenskap.
|
|
| 25. |
|
|
