| 1. |
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| 2. |
- Saveer, Ahmed, et al.
(author)
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A herbivore-induced plant volatile interferes with host plant and mate location in moths through suppression of olfactory signalling pathways
- 2015
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In: BMC Biology. - : Springer Science and Business Media LLC. - 1741-7007. ; 13
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Journal article (peer-reviewed)abstract
- Background: Plants under herbivore attack release volatiles that attract natural enemies, and herbivores in turn avoid such plants. Whilst herbivore-induced plant volatile blends appeared to reduce the attractiveness of host plants to herbivores, the volatiles that are key in this process and particularly the way in which deterrence is coded in the olfactory system are largely unknown. Here we demonstrate that herbivore-induced cotton volatiles suppress orientation of the moth Spodoptera littoralis to host plants and mates.Results: We found that (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), an induced volatile, is key in herbivore deterrence: DMNT suppressed plant odour-and pheromone-induced behaviours. We then dissected the neurophysiological basis of this interaction. DMNT-responding glomeruli were also activated by other plant compounds, suggesting that S. littoralis possesses no segregated olfactory circuit dedicated exclusively to DMNT. Instead, DMNT suppressed responses to the main pheromone component, (Z)-9-(E)-11-tetradecenyl acetate, and primarily to (Z)-3-hexenyl acetate, a host plant attractant.Conclusion: Our study shows that olfactory sensory inhibition, which has previously been reported without reference to an animal's ecology, can be at the core of coding of ecologically relevant odours. As DMNT attracts natural enemies and deters herbivores, it may be useful in the development or enhancement of push-pull strategies for sustainable agriculture.
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| 3. |
- Zakir, Ali, et al.
(author)
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Behavioural Identification of Electrophysiologically Active Compounds From Damaged Cotton Affecting Oviposition Decision in Female Spodoptera littoralis
- 2010
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Conference paper (other academic/artistic)abstract
- Nocturnal insects rely on olfaction for selection of a suitable site for oviposition. Female moths of Spodoptera littoralis have shown oviposition avoidance from caterpillar damaged cotton plant – Gossypium hirsutum – patches in dual choice bioassay. Night headspace volatile analyses showed that larval damaged cotton plants tend to emit a complex mixture of induced compounds belonging to different classes such as: green leaf volatiles (GLVs), terpenoids, as well as cis-jasmone and indole. Gas chromatographic coupled with electroantennographic detection (GC-EAD) studies on headspace collections from damaged cotton plants revealed antennal response to at least eighteen compounds in gravid S. littoralis females. Electroantennographic (EAG) studies on a synthetic mixture of these antennal active coumpounds showed similar response as natural headspace. Further studies are in process to identify the behaviourally active components, affecting the oviposition decision in S. littoralis females, under the application of different synthetic mixtures of the peripherally detected compounds from caterpillar damaged cotton plants
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| 4. |
- Zakir, Ali, et al.
(author)
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Herbivore-Induced Changes in Cotton Modulates Reproductive Behavior in the Moth Spodoptera littoralis
- 2017
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In: Frontiers in Ecology and Evolution. - : Frontiers Media SA. - 2296-701X. ; 5
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Journal article (peer-reviewed)abstract
- Plants produce chemical defense compounds to resist herbivore attack either by repelling the herbivores or attracting natural enemies of the herbivores. We have previously shown that volatile compounds from cotton released in response to herbivory by conspecifics reduce oviposition in cotton leafworm moth Spodoptera littoralis. It remained, however, unclear whether herbivore-induced changes also affect moth pre-mating and mating behaviors. In this study we examined the effect of herbivore-induced changes in cotton on reproductive behaviors i.e., female calling,male attraction and investment, and mating behavior in S. littoralis. We found a reduction in the number of females calling i.e., females releasing pheromone, in the presence of cotton plants damaged by larvae of S. littoralis compared to undamaged plants. Females also spent significantly less time calling and showed a delay in calling in the presence of damaged plants. Furthermore, males exhibited significantly delayed activation and reduced attraction toward female sex pheromone in the presence of damaged plants. We also found that mating success and the number of matings were significantly reduced in the presence of damaged plants whereas male investment i.e., spermatophore weight, was not affected. Thus, our study provides evidence that herbivory by conspecifics on host plants affect pre-mating and mating behaviors in an insect herbivore.
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| 5. |
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| 6. |
- Zakir, Ali, et al.
(author)
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Specific response to herbivore-induced de novo synthesized plant volatiles provides reliable information for host plant selection in a moth
- 2013
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In: Journal of Experimental Biology. - : The Company of Biologists. - 0022-0949 .- 1477-9145. ; 216, s. 3257-3263
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Journal article (peer-reviewed)abstract
- Animals depend on reliable sensory information for accurate behavioural decisions. For herbivorous insects it is crucial to find host plants for feeding and reproduction, and these insects must be able to differentiate suitable from unsuitable plants. Volatiles are important cues for insect herbivores to assess host plant quality. It has previously been shown that female moths of the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae), avoid oviposition on damaged cotton Gossypium hirsutum, which may mediated by herbivore-induced plant volatiles (HIPVs). Among the HIPVs, some volatiles are released following any type of damage while others are synthesized de novo and released by the plants only in response to herbivore damage. In behavioural experiments we here show that oviposition by S. littoralis on undamaged cotton plants was reduced by adding volatiles collected from plants with ongoing herbivory. Gas chromatography–electroantennographic detection (GC–EAD) recordings revealed that antennae of mated S. littoralis females responded to 18 compounds from a collection of headspace volatiles of damaged cotton plants. Among these compounds, a blend of the seven de novo synthesized volatile compounds was found to reduce oviposition in S. littoralis on undamaged plants under both laboratory and ambient (field) conditions in Egypt. Volatile compounds that are not produced de novo by the plants did not affect oviposition. Our results show that ovipositing females respond specifically to the de novo synthesized volatiles released from plants under herbivore attack. We suggest that these volatiles provide reliable cues for ovipositing females to detect plants that could provide reduced quality food for their offspring and an increased risk of competition and predation.
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| 7. |
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| 8. |
- Andreadis, Stefanos, et al.
(author)
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Survey of arthropod assemblages responding to live yeasts in an organic apple orchard
- 2015
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In: Frontiers in Ecology and Evolution. - : Frontiers Media SA. - 2296-701X. ; 3
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Journal article (peer-reviewed)abstract
- Associations between yeasts and insect herbivores are widespread, and these interkingdom interactions play a crucial role in yeast and insect ecology and evolution. We report a survey of insect attraction to live yeast from a community ecology perspective. In the summer of 2013 we screened live yeast cultures of Metschnikowia pulcherrima, M. andauensis, M. hawaiiensis, M. lopburiensis, and Cryptococcus tephrensis in an organic apple orchard. More than 3000 arthropods from 3 classes, 15 orders, and 93 species were trapped; ca. 79% of the trapped specimens were dipterans, of which 43% were hoverflies (Syrphidae), followed by Sarcophagidae, Phoridae, Lauxaniidae, Cecidomyidae, Drosophilidae, and Chironomidae. Traps baited with M. pulcherrima, M. andauensis, and C. tephrensis captured typically 2.4 times more specimens than control traps; traps baited with M. pulcherrima, M. hawaiiensis, M. andauensis, M. lopburiensis, and C. tephrensis were more species-rich than unbaited control traps. We conclude that traps baited with live yeasts of the genera Metschnikowia and Cryprococcus are effective attractants and therefore of potential value for pest control. Yeast-based monitoring or attract-and-kill techniques could target pest insects or enhance the assemblage of beneficial insects. Manipulation of insect behavior through live yeast cultures should be further explored for the development of novel plant protection techniques.
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| 9. |
- Becher, Paul G., et al.
(author)
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Chemical signaling and insect attraction is a conserved trait in yeasts
- 2018
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In: Ecology and Evolution. - : Wiley. - 2045-7758. ; , s. 2962-2974
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Journal article (peer-reviewed)abstract
- Yeast volatiles attract insects, which apparently is of mutual benefit, for both yeasts and insects. However, it is unknown whether biosynthesis of metabolites that attract insects is a basic and general trait, or if it is specific for yeasts that live in close association with insects. Our goal was to study chemical insect attractants produced by yeasts that span more than 250 million years of evolutionary history and vastly differ in their metabolism and lifestyle. We bioassayed attraction of the vinegar fly Drosophila melanogaster to odors of phylogenetically and ecologically distinct yeasts grown under controlled conditions. Baker's yeast Saccharomyces cerevisiae, the insect-associated species Candida californica, Pichia kluyveri and Metschnikowia andauensis, wine yeast Dekkera bruxellensis, milk yeast Kluyveromyces lactis, the vertebrate pathogens Candida albicans and Candida glabrata, and oleophilic Yarrowia lipolytica were screened for fly attraction in a wind tunnel. Yeast headspace was chemically analyzed, and co-occurrence of insect attractants in yeasts and flowering plants was investigated through a database search. In yeasts with known genomes, we investigated the occurrence of genes involved in the synthesis of key aroma compounds. Flies were attracted to all nine yeasts studied. The behavioral response to baker's yeast was independent of its growth stage. In addition to Drosophila, we tested the basal hexapod Folsomia candida (Collembola) in a Y-tube assay to the most ancient yeast, Y. lipolytica, which proved that early yeast signals also function on clades older than neopteran insects. Behavioral and chemical data and a search for selected genes of volatile metabolites underline that biosynthesis of chemical signals is found throughout the yeast clade and has been conserved during the evolution of yeast lifestyles. Literature and database reviews corroborate that yeast signals mediate mutualistic interactions between insects and yeasts. Moreover, volatiles emitted by yeasts are commonly found also in flowers and attract many insect species. The collective evidence suggests that the release of volatile signals by yeasts is a widespread and phylogenetically ancient trait, and that insect-yeast communication evolved prior to the emergence of flowering plants. Co-occurrence of the same attractant signals in yeast and flowers suggests that yeast-insect communication may have contributed to the evolution of insect-mediated pollination in flowers.
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| 10. |
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| 11. |
- Becher, Paul, et al.
(author)
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The Scent of the Fly
- 2018
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In: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 44:5, s. 431-435
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Journal article (peer-reviewed)abstract
- (Z)-4-undecenal (Z4-11Al) is the volatile pheromone produced by females of the vinegar fly Drosophila melanogaster. Female flies emit Z4-11Al for species-specific communication and mate-finding. A sensory panel finds that synthetic Z4-11Al has a characteristic flavour, which can be perceived even at the small amounts produced by a single female fly. Since only females produce Z4-11Al, and not males, we can reliably distinguish between single D. melanogaster males and females, according to their scent. Females release Z4-11Al at 2.4 ng/h and we readily sense 1 ng synthetic Z4-11Al in a glass of wine (0.03 nmol/L), while a tenfold concentration is perceived as a loud off-flavour. This corroborates the observation that a glass of wine is spoilt by a single D. melanogaster fly falling into it, which we here show is caused by Z4-11Al. The biological role of Z4-11Al or structurally related aldehydes in humans and the basis for this semiochemical convergence remains yet unclear.
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| 12. |
- Becher, Paul, et al.
(author)
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Yeast, not fruit volatiles mediate Drosophila melanogaster attraction, oviposition and development
- 2012
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In: Functional Ecology. - : Wiley. - 1365-2435 .- 0269-8463. ; 26:4, s. 822-828
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Journal article (peer-reviewed)abstract
- 1.In nature, the fruit fly Drosophila melanogaster is attracted to fermenting fruit. Micro-organisms like Saccharomyces yeasts growing on fruit occupy a commonly overlooked trophic level between fruit and insects. Although the dietary quality of yeast is well established for D.melanogaster, the individual contribution of fruit and yeast on host finding and reproductive success has not been established. 2.Here, we show that baker's yeast Saccharomyces cerevisiae on its own is sufficient for fruit fly attraction, oviposition and larval development. In contrast, attraction and oviposition were significantly lower if non-fermented grape juice or growth media were used, and yeast-free grapes did not support larval development either. 3.Despite a strong preference for fermented substrates, moderate attraction to and oviposition on unfermented fruit might be adaptive in view of the fly's capacity to vector yeast. 4.Signals emitted by fruit were only of secondary importance because fermenting yeast without fruit induced the same fly behaviour as yeast fermenting on fruit. We identified a synthetic mimic of yeast odour, comprising ethanol, acetic acid, acetoin, 2-phenyl ethanol and 3-methyl-1-butanol, which was as attractive for the fly as fermenting grape juice or fermenting yeast minimal medium. 5.Yeast odours represent the critical signal to establish the flyfruityeast relationship. The traditional plantherbivore niche concept needs to be updated, to accommodate for the role of micro-organisms in insectplant interactions.
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| 13. |
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| 14. |
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| 15. |
- Bengtsson, Marie, et al.
(author)
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Flying the Fly: Long-range Flight Behavior of Drosophila melanogaster to Attractive Odors
- 2010
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In: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 36, s. 599-607
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Journal article (peer-reviewed)abstract
- The fruit fly, Drosophila melanogaster Meigen (Diptera: Drosophilidae), is a model for how animals sense, discriminate, and respond to chemical signals. However, with D. melanogaster our knowledge of the behavioral activity of olfactory receptor ligands has relied largely on close-range attraction, rather than on long-range orientation behavior. We developed a flight assay to relate chemosensory perception to behavior. Headspace volatiles from vinegar attracted 62% of assayed flies during a 15-min experimental period. Flies responded irrespective of age, sex, and mating state, provided they had been starved. To identify behaviorally relevant chemicals from vinegar, we compared the responses to vinegar and synthetic chemicals. Stimuli were applied by a piezoelectric sprayer at known and constant release rates. Re-vaporized methanol extracts of Super Q-trapped vinegar volatiles attracted as many flies as vinegar. The main volatile component of vinegar, acetic acid, elicited significant attraction as a single compound. Two other vinegar volatiles, 2-phenyl ethanol and acetoin, produced a synergistic effect when added to acetic acid. Geosmin, a microbiological off-flavor, diminished attraction to vinegar. This wind tunnel assay based on a conspicuous and unambiguous behavioral response provides the necessary resolution for the investigation of physiologically and ecologically relevant odors and will become an essential tool for the functional analysis of the D. melanogaster olfactory system
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| 16. |
- Bengtsson, Marie, et al.
(author)
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Pheromone races of Cydia splendana (Lepidoptera, Tortricidae) overlap in host plant association and geographic distribution
- 2014
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In: Frontiers in Ecology and Evolution. - : Frontiers Media S.A.. - 2296-701X. ; 2, s. 1-6
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Journal article (peer-reviewed)abstract
- Identification of the sex pheromone of Cydia splendana (Lepidoptera, Tortricidae) bypheromone gland analysis followed by field trapping with synthetic compounds showsthe occurrence of two pheromone races. Acorn moth females from Sweden, whereoak Quercus robur is the only host plant, use a blend of the E,Z and E,E isomers of8,10-dodecadien-1-yl acetate. In Central and Southern Europe, where C. splendana feedson chestnut Castanea sativa and several species of oak, males respond to another isomerblend, E,E and Z,E. The distribution of the two pheromone races of C. splendana overlapsin Northern France, where they share oak as plant host. Differences in sex communicationsignals between these populations of C. splendana corroborate the role of specific materecognition in speciation events.
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| 17. |
- Bengtsson, Marie, et al.
(author)
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Plant odor and sex pheromone are integral elements of specific mate recognition in an insect herbivore
- 2018
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In: Evolution. - : Wiley. - 0014-3820 .- 1558-5646. ; 72, s. 2225-2233
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Journal article (peer-reviewed)abstract
- Specific mate recognition relies on the chemical senses in most animals, and especially in nocturnal insects. Two signal types mediate premating olfactory communication in terrestrial habitats: sex pheromones, which blend into an atmosphere of plant odorants. We show that host plant volatiles affect the perception of sex pheromone in males of the African cotton leafworm Spodoptera littoralis and that pheromone and plant volatiles are not perceived as independent messages. In clean air, S. littoralis males are attracted to single synthetic pheromone components or even the pheromone of a sibling species, oriental cotton leafworm S. litura. Presence of host plant volatiles, however, reduces the male response to deficient or heterospecific pheromone signals. That plant cues enhance discrimination of sex pheromone quality confirms the idea that specific mate recognition in noctuid moths has evolved in concert with adaptation to host plants. Shifts in either female host preference or sex pheromone biosynthesis give rise to new communication channels that have the potential to initiate or contribute to reproductive isolation.
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| 18. |
- Borrero, Felipe, et al.
(author)
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Flight attraction of Spodoptera littoralis (Lepidoptera, Noctuidae) to cotton headspace and synthetic volatile blends.
- 2015
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In: Frontiers in Ecology and Evolution. - : Frontiers Media SA. - 2296-701X. ; 3
-
Journal article (peer-reviewed)abstract
- The insect olfactory system discriminates odor signals of different biological relevance, which drive innate behavior. Identification of stimuli that trigger upwind flight attraction toward host plants is a current challenge, and is essential in developing new, sustainable plant protection methods, and for furthering our understanding of plant-insect interactions. Using behavioral, analytical and electrophysiological studies, we here show that both females and males of the Egyptian cotton leafworm,Spodoptera littoralis(Lepidoptera, Noctuidae), use blends of volatile compounds to locate their host plant, cotton,Gossypium hirsutum(Malvales, Malvaceae). FemaleS. littoraliswere engaged in upwind orientation flight in a wind tunnel when headspace collected from cotton plants was delivered through a piezoelectric sprayer. Although males took off toward cotton headspace significantly fewer males than females flew upwind toward the sprayed headspace. Subsequent assays with antennally active synthetic compounds revealed that a blend of nonanal, (Z)-3 hexenyl acetate, (E)-β-ocimene, and (R)-(+)-limonene was as attractive as cotton headspace to females and more attractive to males. Two compounds, 4,8-dimethyl-1,3(E),7-nonatriene (DMNT) and (R)-(−)-linalool, both known plant defense compounds may have reduced the flight attraction of both females and males; more moths were attracted to blends without these two compounds, however, other compounds such as benzaldehyde may also be behavioral antagonists. Our findings provide a platform for further investigations on host plant signals mediating innate behavior, and for the development of novel insect plant protection strategies againstS. littoralis.
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| 19. |
- Borrero, Felipe, et al.
(author)
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The female sex pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
- 2022
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In: Journal of insect physiology. - : Elsevier BV. - 0022-1910 .- 1879-1611. ; 137
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Journal article (peer-reviewed)abstract
- Specific mate communication and recognition underlies reproduction and hence speciation. Our study provides new insights in Drosophila melanogaster premating olfactory communication. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Female-produced, species-specific volatile pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone (Z)-11-octadecenyl acetate (cVA) interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males. Two isoforms of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. However, only Z4-11Al mediates courtship in experienced males, not (R)-linalool. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds. Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in contrast to Z4-11Al. A blend of the food odour vinegar and cVA attracted females, while a blend of vinegar and female pheromone Z4-11Al attracted males, instead. Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects.
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| 20. |
- Borrero, Felipe, et al.
(author)
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Yeast Volatomes Differentially Affect Larval Feeding in an Insect Herbivore
- 2019
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In: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 85
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Journal article (peer-reviewed)abstract
- Yeasts form mutualistic interactions with insects. Hallmarks of this interaction include provision of essential nutrients, while insects facilitate yeast dispersal and growth on plant substrates. A phylogenetically ancient chemical dialogue coordinates this interaction, where the vocabulary, the volatile chemicals that mediate the insect response, remains largely unknown. Here, we used gas chromatography-mass spectrometry, followed by hierarchical cluster and orthogonal partial least-squares discriminant analyses, to profile the volatomes of six Metschnikowia spp., Cryptococcus nemorosus, and brewer's yeast (Saccharomyces cerevisiae). The yeasts, which are all found in association with insects feeding on foliage or fruit, emit characteristic, species-specific volatile blends that reflect the phylogenetic context. Species specificity of these volatome profiles aligned with differential feeding of cotton leafworm (Spodoprera littoralis) larvae on these yeasts. Bioactivity correlates with yeast ecology; phylloplane species elicited a stronger response than fruit yeasts, and larval discrimination may provide a mechanism for establishment of insect-yeast associations. The yeast volatomes contained a suite of insect attractants known from plant and especially floral headspace, including (Z)-hexenyl acetate, ethyl (2E,4Z)-deca-2,4-dienoate (pear ester), (3E)-4,8-dimethylnona-1,3,7-triene (DMNT), linalool, alpha-terpineol, beta-myrcene, or (E,E)-alpha-farnesene. A wide overlap of yeast and plant volatiles, notably floral scents, further emphasizes the prominent role of yeasts in plant-microbe-insect relationships, including pollination. The knowledge of insect-yeast interactions can be readily brought to practical application, as live yeasts or yeast metabolites mediating insect attraction provide an ample tool-box for the development of sustainable insect management.IMPORTANCE Yeasts interface insect herbivores with their food plants. Communication depends on volatile metabolites, and decoding this chemical dialogue is key to understanding the ecology of insect-yeast interactions. This study explores the volatomes of eight yeast species which have been isolated from foliage, from flowers or fruit, and from plant-feeding insects. These yeasts each release a rich bouquet of volatile metabolites, including a suite of known insect attractants from plant and floral scent. This overlap underlines the phylogenetic dimension of insect-yeast associations, which according to the fossil record long predate the appearance of flowering plants. Volatome composition is characteristic for each species, aligns with yeast taxonomy, and is further reflected by a differential behavioral response of cotton leafworm larvae, which naturally feed on foliage of a wide spectrum of broad-leaved plants. Larval discrimination may establish and maintain associations with yeasts and is also a substrate for designing sustainable insect management techniques.
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| 21. |
- Bosa Ochoa, Carlos Felipe, et al.
(author)
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Respuesta de Tecia solanivora (Lepidoptera: Gelechiidae) a compuestos volátiles de papa, Solanum tuberosum
- 2011
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In: Revista Colombiana De Entomologia. - 0120-0488. ; 37, s. 1-7
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Journal article (peer-reviewed)abstract
- Trampas cebadas con volátiles de plantas como fuente de atrayente pueden ser una herramienta importante para el monitoreo o control de insectos plaga. La polilla guatemalteca,Teciasolanivora, es una plaga limitante en el cultivo de la papa y aún se desconoce su relación con su planta hospedera, la papa. Se estudió el efecto que producen olores de las diferentes estructuras de la planta hospedera en el comportamiento del insecto. También se estudiaron los compuestos metilfenilacetato y sulcatón, dos compuestos volátiles de la planta identificados previamente que son emitidos por las flores y los tubérculos, además de producir respuestas antenales de T.solanivora. Se realizaron ensayos en olfatómetro y en una jaula recubierta con tul con la utilización de trampas de captura. En olfatómetro la estructura que más atrajo a las hembras fue la flor. En la jaula se registró una mayor captura de hembras en trampas cebadas con el compuesto metilfenilacetato a 100μg, y no se observó un efecto sinérgico al combinar los dos compuestos. Los resultados sugieren la evaluación de otros compuestos sintéticos de la planta de papa y sus mezclas para profundizar en el comportamiento de este insecto.
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| 22. |
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| 23. |
- Cattaneo, Alberto Maria, et al.
(author)
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Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones
- 2017
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
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Journal article (peer-reviewed)abstract
- Olfaction plays a dominant role in the mate-finding and host selection behaviours of the codling moth (Cydia pomonella), an important pest of apple, pear and walnut orchards worldwide. Antennal transcriptome analysis revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ORs) CpomOR1, CpomOR3 and CpomOR6a, which belong to the pheromone receptor (PR) lineage, and the co-receptor (CpomOrco). Using heterologous expression, in both Drosophila olfactory sensory neurones and in human embryonic kidney cells, together with electrophysiological recordings and calcium imaging, we characterize the basic physiological and pharmacological properties of these receptors and demonstrate that they form functional ionotropic receptor channels. Both the homomeric CpomOrco and heteromeric CpomOrco + OR complexes can be activated by the common Orco agonists VUAA1 and VUAA3, as well as inhibited by the common Orco antagonists amiloride derivatives. CpomOR3 responds to the plant volatile compound pear ester ethyl-(E, Z)-2,4-decadienoate, while CpomOR6a responds to the strong pheromone antagonist codlemone acetate (E, E)-8,10-dodecadien-1-yl acetate. These findings represent important breakthroughs in the deorphanization of codling moth pheromone receptors, as well as more broadly into insect ecology and evolution and, consequently, for the development of sustainable pest control strategies based on manipulating chemosensory communication.
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| 24. |
- Cattaneo, Alberto Maria, et al.
(author)
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Candidate pheromone receptors of codling moth Cydia pomonella respond to pheromones and kairomones
- 2018
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Conference paper (other academic/artistic)abstract
- Olfaction plays a dominant role in the mate-finding and host selection behaviors of the codling moth (Cydia pomonella), an important pest of apple, pear and walnut orchards. Antennal transcriptome analysis (Bengtsson et al. 2012, Walker et al. 2016) revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ORs) CpomOR1, CpomOR3 and CpomOR6a, which belong to the putative pheromone receptor (PR) lineage, and the co-receptor (CpomOrco). Using heterologous expression, in both human embryonic kidney (HEK293T) cells and in Drosophila olfactory sensory neurons, coupled with calcium imaging and electrophysiological recording, respectively, we characterize the basic physiological and pharmacological properties of these receptors and demonstrate that they form functional ionotropic receptor channels.
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| 25. |
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