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- Pfaller, M.A., et al.
(författare)
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Twelve years of fluconazole in clinical practice : Global-trends in species distribution and fluconazole susceptibility of bloodstream isolates of Candida
- 2004
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Ingår i: Clinical Microbiology and Infection. - : Elsevier BV. - 1198-743X .- 1469-0691. ; 10:SUPPL. 1, s. 11-23
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Tidskriftsartikel (refereegranskat)abstract
- We determined the species distribution and in-vitro susceptibility of 6082 bloodstream infection (BSI) isolates of Candida spp. collected from 250 medical centres in 32 nations over a 10-year period from 1992 through 2001. The species included 3401 C. albicans, 984 C. glabrata, 796 C. parapsilosis, 585 C. tropicalis, 153 C. krusei, 67 C. lusitaniae, 48 C. guilliermondii, 10 C. famata, 10 C. kefyr, six C. pelliculosa, five C. rugosa, four C. lipolytica, three C. dubliniensis, three C. inconspicua, two C. sake and one isolate each of C. lambica, C. norvegensis and C. zeylanoides. Minimum inhibitory concentration determinations were made using the National Committee for Clinical Laboratory Standards reference broth microdilution method. Variation in the rank order and frequency of the different species of Candida was observed over time and by geographic area. The proportion of BSI due to C. albicans and C. glabrata increased and C. parapsilosis decreased over time in Canada, the USA and Europe. C. glabrata was an infrequent cause of BSI in Latin America and the Asia-Pacific region. Very little variation in fluconazole susceptibility was observed among isolates of C. albicans, C. tropicalis and C. parapsilosis. These species accounted for 78% of all BSI and remained highly susceptible (91-100% susceptible) to fluconazole from 1992 to 2001 irrespective of geographic origin. The prevalence of fluconazole resistance among C. glabrata isolates was variable both over time and among the various countries and regions. Resistance to fluconazole among C. glabrata isolates was greatest in the USA and varied by US census region (range 0-23%). These observations are generally encouraging relative to the sustained usefulness of fluconazole as a systemically active antifungal agent for the treatment of candida BSI. © 2004 Copyright by the European Society of Clinical Microbiology and Infectious Diseases.
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- Bruin, WB, et al.
(författare)
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Structural neuroimaging biomarkers for obsessive-compulsive disorder in the ENIGMA-OCD consortium: medication matters
- 2020
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Ingår i: Translational psychiatry. - : Springer Science and Business Media LLC. - 2158-3188. ; 10:1, s. 342-
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Tidskriftsartikel (refereegranskat)abstract
- No diagnostic biomarkers are available for obsessive-compulsive disorder (OCD). Here, we aimed to identify magnetic resonance imaging (MRI) biomarkers for OCD, using 46 data sets with 2304 OCD patients and 2068 healthy controls from the ENIGMA consortium. We performed machine learning analysis of regional measures of cortical thickness, surface area and subcortical volume and tested classification performance using cross-validation. Classification performance for OCD vs. controls using the complete sample with different classifiers and cross-validation strategies was poor. When models were validated on data from other sites, model performance did not exceed chance-level. In contrast, fair classification performance was achieved when patients were grouped according to their medication status. These results indicate that medication use is associated with substantial differences in brain anatomy that are widely distributed, and indicate that clinical heterogeneity contributes to the poor performance of structural MRI as a disease marker.
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- Bandelow, B., et al.
(författare)
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World Federation of Societies of Biological Psychiatry (WFSBP) guidelines for treatment of anxiety, obsessive-compulsive and posttraumatic stress disorders - Version 3. Part II: OCD and PTSD
- 2023
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Ingår i: World Journal of Biological Psychiatry. - : Informa UK Limited. - 1562-2975 .- 1814-1412. ; 24:2, s. 118-134
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Tidskriftsartikel (refereegranskat)abstract
- Aim: This is the third version of the guideline of the World Federation of Societies of Biological Psychiatry (WFSBP) Task Force for the Pharmacological Treatment of Anxiety, Obsessive-Compulsive and Posttraumatic Stress Disorders which was published in 2002 and revised in 2008. Method: A consensus panel of 34 international experts representing 22 countries developed recommendations based on efficacy and acceptability of the treatments. In this version, not only medications but also psychotherapies and other non-pharmacological interventions were evaluated, applying the same rigorous methods that are standard for the assessment of medication treatments. Result: The present paper (Part II) contains recommendations based on published randomised controlled trials (RCTs) for the treatment of OCD (n = 291) and PTSD (n = 234) in children, adolescents, and adults. The accompanying paper (Part I) contains the recommendations for the treatment of anxiety disorders. For OCD, first-line treatments are selective serotonin reuptake inhibitors (SSRIs) and cognitive behavioural therapy (CBT). Internet-CBT was also superior to active controls. Several second-line medications are available, including clomipramine. For treatment-resistant cases, several options are available, including augmentation of SSRI treatment with antipsychotics and other drugs. Other non-pharmacological treatments, including repetitive transcranial magnetic stimulation (rTMS), deep brain stimulation (DBS) and others were also evaluated. For PTSD, SSRIs and the SNRI venlafaxine are first-line treatments. CBT is the psychotherapy modality with the best body of evidence. For treatment-unresponsive patients, augmentation of SSRI treatment with antipsychotics may be an option. Conclusion: OCD and PTSD can be effectively treated with CBT and medications.
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- Bandelow, B., et al.
(författare)
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World Federation of Societies of Biological Psychiatry (WFSBP) guidelines for treatment of anxiety, obsessive-compulsive and posttraumatic stress disorders - Version 3. Part I: Anxiety disorders
- 2023
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Ingår i: World Journal of Biological Psychiatry. - : Informa UK Limited. - 1562-2975 .- 1814-1412. ; 24:2, s. 118-134
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Tidskriftsartikel (refereegranskat)abstract
- Aim This is the third version of the guideline of the World Federation of Societies of Biological Psychiatry (WFSBP) Task Force for the Pharmacological Treatment of Anxiety, Obsessive-Compulsive and Posttraumatic Stress Disorders (published in 2002, revised in 2008). Method A consensus panel of 33 international experts representing 22 countries developed recommendations based on efficacy and acceptability of available treatments. In total, 1007 RCTs for the treatment of these disorders in adults, adolescents, and children with medications, psychotherapy and other non-pharmacological interventions were evaluated, applying the same rigorous methods that are standard for the assessment of medications. Result This paper, Part I, contains recommendations for the treatment of panic disorder/agoraphobia (PDA), generalised anxiety disorder (GAD), social anxiety disorder (SAD), specific phobias, mixed anxiety disorders in children and adolescents, separation anxiety and selective mutism. Selective serotonin reuptake inhibitors (SSRI) and serotonin-norepinephrine reuptake inhibitors (SNRIs) are first-line medications. Cognitive behavioural therapy (CBT) is the first-line psychotherapy for anxiety disorders. The expert panel also made recommendations for patients not responding to standard treatments and recommendations against interventions with insufficient evidence. Conclusion It is the goal of this initiative to provide treatment guidance for these disorders that has validity throughout the world.
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- Weeland, CJ, et al.
(författare)
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The thalamus and its subnuclei-a gateway to obsessive-compulsive disorder
- 2022
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Ingår i: Translational psychiatry. - : Springer Science and Business Media LLC. - 2158-3188. ; 12:1, s. 70-
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Tidskriftsartikel (refereegranskat)abstract
- Larger thalamic volume has been found in children with obsessive-compulsive disorder (OCD) and children with clinical-level symptoms within the general population. Particular thalamic subregions may drive these differences. The ENIGMA-OCD working group conducted mega- and meta-analyses to study thalamic subregional volume in OCD across the lifespan. Structural T1-weighted brain magnetic resonance imaging (MRI) scans from 2649 OCD patients and 2774 healthy controls across 29 sites (50 datasets) were processed using the FreeSurfer built-in ThalamicNuclei pipeline to extract five thalamic subregions. Volume measures were harmonized for site effects using ComBat before running separate multiple linear regression models for children, adolescents, and adults to estimate volumetric group differences. All analyses were pre-registered (https://osf.io/73dvy) and adjusted for age, sex and intracranial volume. Unmedicated pediatric OCD patients (<12 years) had larger lateral (d = 0.46), pulvinar (d = 0.33), ventral (d = 0.35) and whole thalamus (d = 0.40) volumes at unadjusted p-values <0.05. Adolescent patients showed no volumetric differences. Adult OCD patients compared with controls had smaller volumes across all subregions (anterior, lateral, pulvinar, medial, and ventral) and smaller whole thalamic volume (d = −0.15 to −0.07) after multiple comparisons correction, mostly driven by medicated patients and associated with symptom severity. The anterior thalamus was also significantly smaller in patients after adjusting for thalamus size. Our results suggest that OCD-related thalamic volume differences are global and not driven by particular subregions and that the direction of effects are driven by both age and medication status.
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- Yun, JY, et al.
(författare)
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Corrigendum
- 2020
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Ingår i: Brain : a journal of neurology. - : Oxford University Press (OUP). - 1460-2156. ; 143:5, s. e44-
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Tidskriftsartikel (refereegranskat)
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25. |
- Abdellaoui, A, et al.
(författare)
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Phenome-wide investigation of health outcomes associated with genetic predisposition to loneliness
- 2019
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Ingår i: Human molecular genetics. - : Oxford University Press (OUP). - 1460-2083 .- 0964-6906. ; 28:22, s. 3853-3865
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Tidskriftsartikel (refereegranskat)abstract
- Humans are social animals that experience intense suffering when they perceive a lack of social connection. Modern societies are experiencing an epidemic of loneliness. Although the experience of loneliness is universally human, some people report experiencing greater loneliness than others. Loneliness is more strongly associated with mortality than obesity, emphasizing the need to understand the nature of the relationship between loneliness and health. Although it is intuitive that circumstantial factors such as marital status and age influence loneliness, there is also compelling evidence of a genetic predisposition toward loneliness. To better understand the genetic architecture of loneliness and its relationship with associated outcomes, we extended the genome-wide association study meta-analysis of loneliness to 511 280 subjects, and detect 19 significant genetic variants from 16 loci, including four novel loci, as well as 58 significantly associated genes. We investigated the genetic overlap with a wide range of physical and mental health traits by computing genetic correlations and by building loneliness polygenic scores in an independent sample of 18 498 individuals with EHR data to conduct a PheWAS with. A genetic predisposition toward loneliness was associated with cardiovascular, psychiatric, and metabolic disorders and triglycerides and high-density lipoproteins. Mendelian randomization analyses showed evidence of a causal, increasing, the effect of both BMI and body fat on loneliness. Our results provide a framework for future studies of the genetic basis of loneliness and its relationship to mental and physical health.
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- Brewer, Judson, et al.
(författare)
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Interventions and Implications
- 2021
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Ingår i: Intrusive Thinking. - : MIT Press. - 9780262542371 ; , s. 347-377
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Bokkapitel (refereegranskat)
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28. |
- Denys, R. V., et al.
(författare)
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Crystal chemistry and thermodynamic properties of anisotropic Ce2Ni7H4.7 hydride
- 2007
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Ingår i: Journal of Solid State Chemistry. - : Elsevier BV. - 0022-4596 .- 1095-726X. ; 180:9, s. 2566-2576
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Tidskriftsartikel (refereegranskat)abstract
- A new intermetallic deuteride Ce2Ni7D4.7 with an anomalous volume expansion has been studied. Its structure was solved on the basis of in situ neutron diffraction data. Expansion proceeds along the c-axis and within the CeNi2 slabs only. All D atoms are located inside these slabs and on the border between CeNi2 and CeNi5. Ordering of D atoms in the bulk of CeNi2 is accompanied by substantial deformation of these slabs thus lowering the hexagonal symmetry to orthorhombic [space group Pmcn (No. 62); a = 4.9251(3) angstrom b = 8.4933(4) angstrom, c = 29.773(1) angstrom]. Inside the CeNi2 layer the hydrogen sublattice is completely ordered; all D-D distances exceed 2.0 angstrom Local coordination of Ni by D inside the CeNi, blocks is of "open", saddle-like type. Hydrogen ordering is mainly determined by Ce-H and H-H interactions. The press ure-composition-temperature measurements yielded the following thermodynamic parameters of the formation of the hydride: Delta H = -22.4 kJ/MOl(H), Delta S = -59.9 J/(K mol(H)).
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29. |
- Denys, R. V., et al.
(författare)
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Mg substitution effect on the hydrogenation behaviour, thermodynamic and structural properties of the La2Ni7-H(D)(2) system
- 2008
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Ingår i: Journal of Solid State Chemistry. - : Elsevier BV. - 0022-4596 .- 1095-726X. ; 181:4, s. 812-821
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Tidskriftsartikel (refereegranskat)abstract
- The present work is focused on studies of the influence of magnesium on the hydrogenation behaviour of the (La,Mg)(2)Ni-7 alloys. Substitution of La in La2Ni7 by Mg to form La1.5Mg0.5Ni7 preserves the initial Ce2Ni7 type of the hexagonal P6(3)/mmc structure and leads to contraction of the unit cell. The system La1.5Mg0.5Ni7-H2 (D-2) was studied using in situ synchrotron X-ray and neutron powder diffraction in H-2/D-2 gas and pressure-composition-temperature measurements. La replacement by Mg was found to proceed in an ordered way, only within the Laves-type parts of the hybrid crystal structure, yielding formation of LaMgNi4 slabs with statistic and equal occupation of one site by La and Mg atoms. Mg alters structural features of the hydrogenation process. Instead of a strong unilateral anisotropic expansion which takes place on hydrogenation of La2Ni7, the unit cell of La1.5Mg0.5Ni7D9.1 is formed by nearly equal hydrogen-induced expansions proceeding in the basal plane (Delta a/a = 7.37%) and along [001] (Delta c/c = 9.67%). In contrast with La2Ni7D6.5 where only LaNi2 layers absorb hydrogen atoms, in La1.5Mg0.5Ni7D9.1 both LaNi5 and LaMgNi4 layers become occupied. Nine types of sites were found to be filled by D in total, including tetrahedral (La,Mg)(2)Ni-2, (La,Mg)Ni-3, Ni-4, tetragonal pyramidal La2Ni3 and trigonal bipyramidal (La,Mg)(3)Ni-2 interstices. The hydrogen sublattice around the La/Mg site shows formation of two co-ordination spheres of D atoms: an octahedron MgD6 and a 16-vertex polyhedron LaD16 around La. The interatomic distances are in the following ranges: La-D (2.28-2.71), Mg-D (2.02-2.08), Ni-D (1.48-1.86 angstrom). All D-D distances exceed 1.9 angstrom. Thermodynamic PCT studies yielded the following values for the Delta H and Delta S of hydrogenation/decomposition; Delta H-H = -15.7 +/- 0.9 kJ (mol(H))(-1) and Delta S-H = -46.0 +/- 3.7 J (K mol(H))(-1) for H-2 absorption, and Delta H-H = 16.8 +/- 0.4 kJ (mol(H))(-1) and Delta S-H = 48.1 +/- 1.5 J (K mol(H))(-1) for H-2 desorption.
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30. |
- Visser-Vandewalle, Veerle, et al.
(författare)
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Deep brain stimulation for obsessive-compulsive disorder : a crisis of access
- 2022
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Ingår i: Nature Medicine. - : Nature Publishing Group. - 1078-8956 .- 1546-170X. ; 28:8, s. 1529-1532
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Tidskriftsartikel (refereegranskat)abstract
- Deep brain stimulation is an effective treatment for obsessive–compulsive disorder but is rarely used. Action is needed by psychologists, psychiatrists and insurers so that patients with otherwise intractable cases can receive this therapy to improve their mental health.
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31. |
- Ahlstrom, Christina A., et al.
(författare)
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Genomically diverse carbapenem resistant Enterobacteriaceae from wild birds provide insight into global patterns of spatiotemporal dissemination
- 2022
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Ingår i: Science of the Total Environment. - : Elsevier. - 0048-9697 .- 1879-1026. ; 824
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Tidskriftsartikel (refereegranskat)abstract
- Carbapenem resistant Enterobacteriaceae (CRE) are a threat to public health globally, yet the role of the environment in the epidemiology of CRE remains elusive. Given that wild birds can acquire CRE, likely from foraging in anthropogenically impacted areas, and may aid in the maintenance and dissemination of CRE in the environment, a spatiotemporal comparison of isolates from different regions and timepoints may be useful for elucidating epidemiological information. Thus, we characterized the genomic diversity of CRE from fecal samples opportunistically collected from gulls (Larus spp.) inhabiting Alaska (USA), Chile, Spain, Turkey, and Ukraine and from black kites (Milvus migrans) sampled in Pakistan and assessed evidence for spatiotemporal patterns of dissemination. Within and among sampling locations, a high diversity of carbapenemases was found, including Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-beta-lactamase (NDM), oxacillinase (OXA), and Verona integron Metallo beta-lactamase (VIM). Although the majority of genomic comparisons among samples did not provide evidence for spatial dissemination, we did find strong evidence for dissemination among Alaska, Spain, and Turkey. We also found strong evidence for temporal dissemination among samples collected in Alaska and Pakistan, though the majority of CRE clones were transitory and were not repeatedly detected among locations where samples were collected longitudinally. Carbapenemase-producing hypervirulent K. pneumoniae was isolated from gulls in Spain and Ukraine and some isolates harbored antimicrobial resistance genes conferring resistance to up to 10 different antibiotic classes, including colistin. Our results are consistent with local acquisition of CRE by wild birds with spatial dissemination influenced by intermediary transmission routes, likely involving humans. Furthermore, our results support the premise that anthropogenicallyassociated wild birds may be good sentinels for understanding the burden of clinically-relevant antimicrobial resistance in the local human population.
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34. |
- Denys, R. V., et al.
(författare)
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Hydrogen storage properties and structure of La1-xMgx(Ni1-yMny)(3) intermetallics and their hydrides
- 2007
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Ingår i: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388 .- 1873-4669. ; 446, s. 166-172
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Tidskriftsartikel (refereegranskat)abstract
- 'Hybrid' RNi3 (R =rare earth metal) crystal structures are built of the slabs of simpler types, CaCu5 and MgZn2. Different affinities of these slabs to hydrogen result in unusual "anisotropic" expansion of the RNi3 and R2Ni7 (R = La, Cc) structures upon hydrogenation. This work focuses on studies of the hydrogenation behaviour of LaNi3 and on the properties of the hydrides of the modified by Mg and Mn La-Ni alloys. The crystal structure of LaNi3D2.8 and the crystal structure and hydrogen storage behaviours of the La1.5Mg0.5Ni7 and La(Ni-1-,Mn,)3 (x=0; 0.067-, 0.133; 0.2: 0.267; 0.3; 0.333; 0.4) alloys were in focus. The deuteration of LaNi3 with PuNi3 type of structure leads to the formation of LaNi3D2.8 and is accompanied by a deformation of the metal matrix causing a change of the initial rhombohedral symmetry (space group R3m) to a nionoclinic one (space group C2/m; a = 8.6408(7) angstrom, b=4.928](4) angstrom, c= 32.774(3) A; = 90.850(8)degrees; V= 1395.5(2) angstrom(3)). Similar to the earlier studied CeNi3D2.8, preferential occupation by deuterium atoms of the AB, layers takes place, leading to the "anisotropic" expansion of the unit cell along [0 0 1] (Delta c/c = 30.6%). 14 occupied D crystallographic sites have 4 chemically different types of metal-atom surroundings, including Ni-4 (2), La2Ni2 (2), La3Ni (6), and La3Ni3 (4). Modification of the La-Ni alloys by magnesium and manganese leads to the formation of intermetallic compounds crystallising with the PuNi3, CeNi3, and Ce2Ni7-type structures. An ordered substitution of La by Mg in the MgZn2-type slabs was observed, causing a complete alteration of the hydrogenation behaviour of the original LaNi3 alloy. La1.5Mg0.5Ni7D9 isotropically expands upon its formation and leads to a substantial increase of the stability against hydrogenation-induced aniorphisation. On the other hand, replacement of Ni by Mn leads to the change in crystal-structure type from PuNi3 to CeNi3 in the LaNi3-xMn. alloys (x>0.1). An ordered substitution of Ni by Mn proceeds inside the RNi5 slabs only. This decreases the stability of the initial alloy against amorphisation on hydrogenation.
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36. |
- Hirscher, Michael, et al.
(författare)
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Materials for hydrogen-based energy storage - past, recent progress and future outlook
- 2020
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Ingår i: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388 .- 1873-4669. ; 827
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Tidskriftsartikel (refereegranskat)abstract
- Globally, the accelerating use of renewable energy sources, enabled by increased efficiencies and reduced costs, and driven by the need to mitigate the effects of climate change, has significantly increased research in the areas of renewable energy production, storage, distribution and end-use. Central to this discussion is the use of hydrogen, as a clean, efficient energy vector for energy storage. This review, by experts of Task 32, Hydrogen-based Energy Storage of the International Energy Agency, Hydrogen TCP, reports on the development over the last 6 years of hydrogen storage materials, methods and techniques, including electrochemical and thermal storage systems. An overview is given on the background to the various methods, the current state of development and the future prospects. The following areas are covered; porous materials, liquid hydrogen carriers, complex hydrides, intermetallic hydrides, electrochemical storage of energy, thermal energy storage, hydrogen energy systems and an outlook is presented for future prospects and research on hydrogen-based energy storage.
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38. |
- Kravtsov, Vadim, et al.
(författare)
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Orbital variability of the optical linear polarization of the gamma-ray binary LS I+61 degrees 303 and new constraints on the orbital parameters
- 2020
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 643
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Tidskriftsartikel (refereegranskat)abstract
- We studied the variability of the linear polarization and brightness of the gamma -ray binary LS I +61 degrees 303. High-precision BVR photopolarimetric observations were carried out with the Dipol-2 polarimeter on the 2.2 m remotely controlled UH88 telescope at Mauna Kea Observatory and the 60 cm Tohoku telescope at Haleakala bservatory (Hawaii) over 140 nights in 2016-2019. We also determined the degree and angle of the interstellar polarization toward LS I +61 degrees 303 using two out of four nearby field stars that have Gaia's parallaxes. After subtracting the interstellar polarization, we determined the position angle of the intrinsic polarization theta similar or equal to 11 degrees, which can either be associated with the projection of the Be star's decretion disk axis on the plane of sky, or can differ from it by 90 degrees. Using the Lomb-Scargle method, we performed timing analyses and period searches of our polarimetric and photometric data. We found statistically significant periodic variability of the normalized Stokes parameters q and u in all passbands. The most significant period of variability, P-Pol=13.244 +/- 0.012 d, is equal to one half of the orbital period P-orb=26.496 d. The fits of the polarization variability curves with Fourier series show a dominant contribution from the second harmonic which is typical for binary systems with circular orbits and nearly symmetric distribution of light scattering material with respect to the orbital plane. The continuous change of polarization with the orbital phase implies co-planarity of the orbit of the compact object and the Be star's decretion disk. Using a model of Thomson scattering by a cloud that orbits the Be star, we obtained constraints on the orbital parameters, including a small eccentricity e<0.2 and periastron phase of (p)approximate to 0.6, which coincides with the peaks in the radio, X-ray, and TeV emission. These constraints are independent of the assumption about the orientation of the decretion disk plane on the sky. We also extensively discuss the apparent inconsistency with the previous measurements of the orbital parameters from radial velocities. By folding the photometry data acquired during a three-year time span with the orbital period, we found a linear phase shift of the moments of the brightness maximum, confirming the possible existence of superorbital variability.
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39. |
- Marushchak, Denys, 1981-
(författare)
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Fluorescence studies of complex systems : organisation of biomolecules
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The homo and hetero dimerisation of two spectroscopically different chromophores were studied, namely: 4,4-difluoro-4-bora-3a,4a-diazas-indacene (g-BODIPY) and its 5-styryl-derivative (r-BODIPY). Various spectroscopic properties of the r-BODIPY in different common solvents were determined. It was shown that g- and r-BODIPY in the ground state can form homo- as well as hetero dimers. We demonstrate that the ganglioside GM1 in lipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibits a non-uniform lateral distribution, which is an argument in favour of self-aggregation of GM1 being an intrinsic property of the GM1. This was concluded from energy transfer/migration studies of BODIPY-labelled gangliosides. An algorithm is presented that quantitatively accounts for donor–donor energy migration (DDEM) among fluorophore-labelled proteins forming regular non-covalent polymers. The DDEM algorithm is based on Monte Carlo (MC) and Brownian dynamics (BD) simulations and applies to the calculation of fluorescence depolarisation data, such as the fluorescence anisotropy. Thereby local orientations, as well as reorienting motions of the fluorescent groups are considered in the absence and presence of DDEM among them. A new method, in which a genetic algorithm (GA) was combined with BD and MC simulations, was developed to analyse fluorescence depolarisation data collected by the time-correlated single photon counting technique. It was applied to study g-BODIPY-labelled filamentous actin (F-actin). The technique registered the local order and reorienting motions of the fluorophores, which were covalently coupled to cysteine 374 (C374) in actin and interacted by means of electronic energy migration within the polymer. Analyses of F-actin samples composed of different fractions of labelled actin molecules revealed the known helical organiszation of F-actin, and demonstrated the usefulness of this technique for structure determination of complex protein polymers. The distance from the filament axis to the fluorophore was found to be considerably less than expected from the proposed position of C374 at a high filament radius. In addition, polymerisation experiments with BODIPY-actin suggest a 25-fold more efficient signal for filament formation than pyrene-actin.
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40. |
- Marushchak, Denys, et al.
(författare)
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On the quantitative treatment of donor-donor energy migration in regularly aggregated proteins
- 2005
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Ingår i: Journal of Fluorescence. - New York : Plenum Press. - 1053-0509 .- 1573-4994. ; 15:5, s. 797-803
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Tidskriftsartikel (refereegranskat)abstract
- An algorithm is presented that quantitatively accounts for donor–donor energy migration (DDEM) among fluorophore-labeled proteins forming regular aggregates. The DDEM algorithm is based on Monte Carlo and Brownian dynamics simulations and applies to calculation of fluorescence depolarisation data, such as the fluorescence anisotropy. Thereby local orientations, as well as reorienting motions of the fluorescent group are considered in the absence and presence of DDEM and among, in principle, infinitely many proteins as they form regular aggregates. Here we apply the algorithm for calculating and illustrating the DDEM and the time-resolved fluorescence anisotropy under static as well as dynamic conditions within helical, linear and circular aggregate structures. A principal approach of the DDEM algorithm for analysing protein aggregates is also outlined.
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41. |
- Nazer, N. S., et al.
(författare)
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In operando neutron diffraction study of LaNdMgNi9H13 as a metal hydride battery anode
- 2017
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Ingår i: Journal of Power Sources. - : Elsevier BV. - 0378-7753. ; 343, s. 502-512
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Tidskriftsartikel (refereegranskat)abstract
- La2MgNi9-related alloys are superior metal hydride battery anodes as compared to the commercial AB5 alloys. Nd-substituted La2-yNdyMgNi9 intermetallics are of particular interest because of increased diffusion rate of hydrogen and thus improved performance at high discharge currents. The present work presents in operando characterization of the LaNdMgNi9 intermetallic as anode for the nickel metal hydride (Ni-MH) battery. We have studied the structural evolution of LaNdMgNi9 during its charge and discharge using in situ neutron powder diffraction. The work included experiments using deuterium gas and electrochemical charge-discharge measurements. The alloy exhibited a high electrochemical discharge capacity (373 mAh/g) which is 20% higher than the AB5 type alloys. A saturated β-deuteride synthesized by solid-gas reaction at PD2 = 1.6 MPa contained 12.9 deuterium atoms per formula unit (D/f.u.) which resulted in a volume expansion of 26.1%. During the electrochemical charging, the volume expansion (23.4%) and D-contents were found to be slightly reduced. The reversible electrochemical cycling is performed through the formation of a two-phase mixture of the α-solid solution and β-hydride phases. Nd substitution contributes to the high-rate dischargeability, while maintaining a good cyclic stability. Electrochemical Impedance Spectroscopy (EIS) was used to characterize the anode electrode on cycling. A mathematical model for the impedance response of a porous electrode was utilized. The EIS showed a decreased hydrogen transport rate during the long-term cycling, which indicated a corresponding slowing down of the electrochemical processes at the surface of the metal hydride anode.
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42. |
- Pasquini, Luca, et al.
(författare)
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Magnesium- and intermetallic alloys-based hydrides for energy storage : modelling, synthesis and properties
- 2022
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Ingår i: Progress in Energy. - : Institute of Physics Publishing (IOPP). - 2516-1083. ; 4:3
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Forskningsöversikt (refereegranskat)abstract
- Hydrides based on magnesium and intermetallic compounds provide a viable solution to the challenge of energy storage from renewable sources, thanks to their ability to absorb and desorb hydrogen in a reversible way with a proper tuning of pressure and temperature conditions. Therefore, they are expected to play an important role in the clean energy transition and in the deployment of hydrogen as an efficient energy vector. This review, by experts of Task 40 'Energy Storage and Conversion based on Hydrogen' of the Hydrogen Technology Collaboration Programme of the International Energy Agency, reports on the latest activities of the working group 'Magnesium- and Intermetallic alloys-based Hydrides for Energy Storage'. The following topics are covered by the review: multiscale modelling of hydrides and hydrogen sorption mechanisms; synthesis and processing techniques; catalysts for hydrogen sorption in Mg; Mg-based nanostructures and new compounds; hydrides based on intermetallic TiFe alloys, high entropy alloys, Laves phases, and Pd-containing alloys. Finally, an outlook is presented on current worldwide investments and future research directions for hydrogen-based energy storage.
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43. |
- Santos, Denys E. S., et al.
(författare)
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Conformational Dynamics and Responsiveness of Weak and Strong Polyelectrolyte Brushes : Atomistic Simulations of Poly(dimethyl aminoethyl methacrylate) and Poly(2-(methacryloyloxy)ethyl trimethylammonium chloride)
- 2019
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Ingår i: Langmuir. - Washington : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 35:14, s. 5037-5049
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Tidskriftsartikel (refereegranskat)abstract
- The complex solution behavior of polymer brushes is key to control their properties, including for biomedical applications and catalysis. The swelling behavior of poly(dimethyl aminoethyl methacrylate) (PDMAEMA) and poly(2-(methacryloyloxy)ethyl trimethylammonium chloride) (PMETAC) in response to changes in pH, solvent, and salt types has been investigated using atomistic molecular dynamics simulations. PDMAEMA and PMETAC have been selected as canonical models for weak and strong polyelectrolytes whose complex conformational behavior is particularly challenging for the development and validation of atomistic models. The GROMOS-derived atomic parameters reproduce the experimental swelling coefficients obtained from ellipsometry measurements for brushes of 5–15 nm thickness. The present atomistic models capture the protonated morphology of PDMAEMA, the swollen and collapsed conformations of PDMAEMA and PMETAC in good and bad solvents, and the salt-selective response of PMETAC. The modular nature of the molecular models allows for the simple extension of atomic parameters to a variety of polymers or copolymers.
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44. |
- Santos, Denys E. S., et al.
(författare)
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Polymyxin Binding to the Bacterial Outer Membrane Reveals Cation Displacement and Increasing Membrane Curvature in Susceptible but Not in Resistant Lipopolysaccharide Chemotypes
- 2017
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Ingår i: Journal of Chemical Information and Modeling. - : AMER CHEMICAL SOC. - 1549-9596 .- 1549-960X. ; 57:9, s. 2181-2193
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Tidskriftsartikel (refereegranskat)abstract
- Lipid-A is the causative agent of Gram-negative sepsis and is responsible for an increasingly high mortality rate among hospitalized patients. Compounds that bind Lipid-A can limit this inflammatory process. The cationic antimicrobial peptide polymyxin B (Pmx-B) is one of the simplest molecules capable of selectively binding to Lipid-A and may serve as a model for further development of Lipid-A binding agents. Gram-negative bacteria resistance to Pmx-B relies on the upregulation of a number of regulatory systems, which promote chemical modifications of the lipopolysaccharide (LPS) structure and leads to major changes in the physical chemical properties of the outer membrane. A detailed understanding of how the chemical structure of the LPS modulates macroscopic properties of the outer membrane is paramount for the design and optimization of novel drugs targeting clinically relevant strains. We have performed a systematic investigation of Pmx-B binding to outer membrane models composed of distinct LPS chemotypes experimentally shown to be either resistant or susceptible to the peptide. Molecular dynamics simulations were carried out for Pmx-B bound to the penta- and hexa-acylated forms of Lipid-A (more susceptible) and Lipid-A modified with 4-amino-4-deoxy-L-arabinose (resistant) as well as the penta-acylated form of LPS Re (less susceptible). The present simulations show that upon binding to the bacterial outer membrane surface, Pmx-B promotes cation displacement and structural changes in membrane curvature and integrity as a function of the LPS chemotype susceptibility or resistance to the antimicrobial peptide.
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45. |
- Volkov, Oleksii M., et al.
(författare)
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Three-dimensional magnetic nanotextures with high-order vorticity in soft magnetic wireframes
- 2024
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- Additive nanotechnology enable curvilinear and three-dimensional (3D) magnetic architectures with tunable topology and functionalities surpassing their planar counterparts. Here, we experimentally reveal that 3D soft magnetic wireframe structures resemble compact manifolds and accommodate magnetic textures of high order vorticity determined by the Euler characteristic, χ. We demonstrate that self-standing magnetic tetrapods (homeomorphic to a sphere; χ = + 2) support six surface topological solitons, namely four vortices and two antivortices, with a total vorticity of + 2 equal to its Euler characteristic. Alternatively, wireframe structures with one loop (homeomorphic to a torus; χ = 0) possess equal number of vortices and antivortices, which is relevant for spin-wave splitters and 3D magnonics. Subsequent introduction of n holes into the wireframe geometry (homeomorphic to an n-torus; χ < 0) enables the accommodation of a virtually unlimited number of antivortices, which suggests their usefulness for non-conventional (e.g., reservoir) computation. Furthermore, complex stray-field topologies around these objects are of interest for superconducting electronics, particle trapping and biomedical applications.
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46. |
- Waldenström, Jonas, 1975-, et al.
(författare)
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Active wild bird surveillance of avian influenza viruses, a report
- 2022
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Ingår i: EFSA Supporting Publications. - : John Wiley & Sons. - 2397-8325 .- 2397-8325. ; 19:12
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This report summarises the potential for an EU-wide active surveillance of highly pathogenic avian influenza (HPAI) virus in wild birds. As basis for the analyses, we collected and analysed virological and ornithological data and carried out fieldwork in two countries outside the EU: Ukraine and Georgia. We show that it is possible to build capacity and logistics for sampling nodes that can provide rapid detection and identification of HPAI, that are key features for an early warning system. Based on the data presented here, it is suggested that an EU-level surveillance network in wild birds is constructed, where surveillance nodes are chosen to reflect the epidemiological benefit of the whole EU. Selection of sampling nodes in the surveillance network should incorporate ornithological and virological background data, and that on-site development should include combined local ornithology and virology expertise and a minimised analytic time frame from sample to result. Ideally, surveillance nodes should be constructed with operational flexibility in what type of material that can be collected, such as active surveillance of wild birds (cloacal, oropharyngeal, blood samples), sampling of hunting bags, and the possibility to take environmental samples or to sample carcasses. This way a surveillance node could be adapted to changes in virus epidemiology, such as shifts in what hosts need to be sampled and at what times of the year sampling should occur. In times of enzootic virus circulation, the network should be tuned to monitor waves of infections and provide warning signals when increased activity is anticipated based on migratory bird movements. Based on the above assumptions, we provide a set of suitable regions, both within and outside the EU, that should be considered.
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47. |
- Yartys, V. A., et al.
(författare)
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Crystal and magnetic structure of TbNiSnD studied by neutron powder diffraction
- 2007
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Ingår i: Journal of Magnetism and Magnetic Materials. - : Elsevier BV. - 0304-8853 .- 1873-4766. ; 311:2, s. 639-643
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Tidskriftsartikel (refereegranskat)abstract
- The crystal and magnetic structure of TbNiSnD has been studied by neutron powder diffraction. The deuteride crystallizes with the filled TiNiSi type structure (sp. gr. Pnma; a=7.0354(1); b=4.20662(9); c=8.1808(2) Å at 293 K; a=7.008(1); b=4.1954(6); c=8.153(1) Å at 2 K) and is canted ferromagnetic below 10 K. The deuterium atoms occupy the tetrahedral Tb3Ni sites. The Tb atoms carry a magnetic moment of 6.6(1) μB /Tb. They order antiferromagnetically along the a-axis and ferromagnetically along the c-axis. No significant magnetic moment on the Ni atoms has been observed by neutron diffraction. Insertion of deuterium in TbNiSn induces both a change of the magnetic structure and a reduction of the Tb magnitude of the magnetic moment.
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48. |
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