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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Algaba, Juan-Carlos, et al.
(författare)
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Broadband Multi-wavelength Properties of M87 during the 2017 Event Horizon Telescope Campaign
- 2021
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Ingår i: Astrophysical Journal Letters. - : American Astronomical Society. - 2041-8213 .- 2041-8205. ; 911:1
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Forskningsöversikt (refereegranskat)abstract
- In 2017, the Event Horizon Telescope (EHT) Collaboration succeeded in capturing the first direct image of the center of the M87 galaxy. The asymmetric ring morphology and size are consistent with theoretical expectations for a weakly accreting supermassive black hole of mass ∼6.5 × 109 M o˙. The EHTC also partnered with several international facilities in space and on the ground, to arrange an extensive, quasi-simultaneous multi-wavelength campaign. This Letter presents the results and analysis of this campaign, as well as the multi-wavelength data as a legacy data repository. We captured M87 in a historically low state, and the core flux dominates over HST-1 at high energies, making it possible to combine core flux constraints with the more spatially precise very long baseline interferometry data. We present the most complete simultaneous multi-wavelength spectrum of the active nucleus to date, and discuss the complexity and caveats of combining data from different spatial scales into one broadband spectrum. We apply two heuristic, isotropic leptonic single-zone models to provide insight into the basic source properties, but conclude that a structured jet is necessary to explain M87's spectrum. We can exclude that the simultaneous γ-ray emission is produced via inverse Compton emission in the same region producing the EHT mm-band emission, and further conclude that the γ-rays can only be produced in the inner jets (inward of HST-1) if there are strongly particle-dominated regions. Direct synchrotron emission from accelerated protons and secondaries cannot yet be excluded.
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| 4. |
- Agrawal, Tarun Kumar, 1989-, et al.
(författare)
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Understanding Supply Chain Visibility Through Experts’ Perspective: A Delphi Based Approach
- 2021
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Ingår i: Advances in Production Management Systems. Artificial Intelligence for Sustainable and Resilient Production Systems. - Cham : Springer Nature. ; 633 IFIP, s. 189-196
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Konferensbidrag (refereegranskat)abstract
- Visibility in production logistics and across the supply chain has become a key concern for organizations. Its need has been further emphasized due to the current COVID 19 crisis. Organizations find it challenging to prepare the internal logistics and supply chain, and quickly respond to such unexpected events, due to low visibility. Against this backdrop, the paper, which is a work-in-progress, systematically documents different factors influencing supply chain visibility and crucial information that should be collected and shared among supply chain partners for better visibility. A Delphi analysis is being conducted with twenty-six supply chain experts from various globally recognized enterprises with manufacturing units located worldwide. The study starts with a short open-ended questioner to collect a comprehensive list of antecedents, drivers, barriers, effects, and visibility information based on the qualitative response from the experts. The preliminary results from the first round of the Delphi analysis indicate that risk management, environmental sustainability, and supply chain control are some of the key drivers. Lack of IT infrastructure and maturity are some of the barriers, integrated systems, and technology maturity are among the key antecedents and gaining planning capability and better customer service are some of the positive effects of supply chain visibility as per the experts’ opinion. In addition, information related to planning, supplier location, and deviation are among the crucial ones that require the collection and sharing for better supply chain visibility. This research study is among the few that empirically explores factors influencing supply chain visibility and generates new insights as to why the barriers can be difficult to overcome in complex supply chain settings.
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| 5. |
- Despeisse, Mélanie, 1985, et al.
(författare)
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Achieving Circular and Efficient Production Systems : Emerging Challenges from Industrial Cases
- 2021
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Ingår i: Advances in Production Management Systems. Artificial Intelligence for Sustainable and Resilient Production Systems. - Cham : Springer. - 1868-4238 .- 1868-422X. - 9783030859091 - 9783030859121 - 9783030859107 ; , s. 523-533
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Konferensbidrag (refereegranskat)abstract
- As the need for more responsible production and consumption grows quickly, so does the interest in the concepts of eco-efficiency and circularity. To make swift progress towards sustainability, solutions must be developed and deployed at scale. It is therefore critical to understand the challenges faced by industry to accelerate the uptake of best practices for circular and efficient production systems. This paper presents the emerging issues from three industrial pilots in an on-going collaborative project. We discuss and suggest further work around crucial questions such as: How to deploy circular solutions from lab to industrial scale? How can digitalization support efficient circular processes?.
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| 6. |
- Kalaiarasan, Ravi, et al.
(författare)
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Requirements on Supply Chain Visibility : A Case on Inbound Logistics
- 2021
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Ingår i: Advances in Production Management Systems. Artificial Intelligence for Sustainable and Resilient Production Systems - IFIP WG 5.7 International Conference, APMS 2021, Proceedings. - Cham : Springer International Publishing. - 1868-422X .- 1868-4238. - 9783030859015 ; 631 IFIP, s. 115-122, s. 115-122
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Konferensbidrag (refereegranskat)abstract
- Events such as Covid-19 have revealed the vulnerabilities that companies face due to low visibility. Consequently, companies experience impact on their supply chains in terms of disruptions of material supply, deliveries, productivity and revenue. Thus, the importance of Supply Chain Visibility (SCV) in global and competitive markets with increasing sustainability demands has received widespread recognition. Yet, the literature provides limited understanding of requirements to consider when developing a SCV system. Addressing the gap, this study presents the findings from a case study during the first months of 2021 at a global manufacturing company developing a SCV system to improve their inbound flow. Using a system engineering perspective, this study presents requirements highlighted during early stage for a SCV system. The results indicate the importance of ensuring SCV system requirements to enable data collection, handling and usage for decision making leading to both supply chain sustainability and resilience. This study contributes to the understanding of SCV by presenting and categorizing requirements considered for real-time case at a manufacturing company when developing a SCV system.
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| 7. |
- Komatsu, Kimberly J., et al.
(författare)
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Global change effects on plant communities are magnified by time and the number of global change factors imposed
- 2019
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 116:36, s. 17867-17873
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Tidskriftsartikel (refereegranskat)abstract
- Accurate prediction of community responses to global change drivers (GCDs) is critical given the effects of biodiversity on ecosystem services. There is consensus that human activities are driving species extinctions at the global scale, but debate remains over whether GCDs are systematically altering local communities worldwide. Across 105 experiments that included over 400 experimental manipulations, we found evidence for a lagged response of herbaceous plant communities to GCDs caused by shifts in the identities and relative abundances of species, often without a corresponding difference in species richness. These results provide evidence that community responses are pervasive across a wide variety of GCDs on long-term temporal scales and that these responses increase in strength when multiple GCDs are simultaneously imposed.Global change drivers (GCDs) are expected to alter community structure and consequently, the services that ecosystems provide. Yet, few experimental investigations have examined effects of GCDs on plant community structure across multiple ecosystem types, and those that do exist present conflicting patterns. In an unprecedented global synthesis of over 100 experiments that manipulated factors linked to GCDs, we show that herbaceous plant community responses depend on experimental manipulation length and number of factors manipulated. We found that plant communities are fairly resistant to experimentally manipulated GCDs in the short term (<10 y). In contrast, long-term (≥10 y) experiments show increasing community divergence of treatments from control conditions. Surprisingly, these community responses occurred with similar frequency across the GCD types manipulated in our database. However, community responses were more common when 3 or more GCDs were simultaneously manipulated, suggesting the emergence of additive or synergistic effects of multiple drivers, particularly over long time periods. In half of the cases, GCD manipulations caused a difference in community composition without a corresponding species richness difference, indicating that species reordering or replacement is an important mechanism of community responses to GCDs and should be given greater consideration when examining consequences of GCDs for the biodiversity–ecosystem function relationship. Human activities are currently driving unparalleled global changes worldwide. Our analyses provide the most comprehensive evidence to date that these human activities may have widespread impacts on plant community composition globally, which will increase in frequency over time and be greater in areas where communities face multiple GCDs simultaneously.
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| 8. |
- Ackermann, M., et al.
(författare)
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THE FIRST FERMI-LAT CATALOG OF SOURCES ABOVE 10 GeV
- 2013
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Ingår i: Astrophysical Journal Supplement Series. - : American Astronomical Society. - 0067-0049 .- 1538-4365. ; 209:2
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Tidskriftsartikel (refereegranskat)abstract
- We present a catalog of gamma-ray sources at energies above 10 GeV based on data from the Large Area Telescope (LAT) accumulated during the first 3 yr of the Fermi Gamma-ray Space Telescope mission. The first Fermi catalog of > 10 GeV sources (1FHL) has 514 sources. For each source we present location, spectrum, a measure of variability, and associations with cataloged sources at other wavelengths. We found that 449 (87%) could be associated with known sources, of which 393 (76% of the 1FHL sources) are active galactic nuclei. Of the 27 sources associated with known pulsars, we find 20 (12) to have significant pulsations in the range > 10 GeV (> 25 GeV). In this work we also report that, at energies above 10 GeV, unresolved sources account for 27% +/- 8% of the isotropic. gamma-ray background, while the unresolved Galactic population contributes only at the few percent level to the Galactic diffuse background. We also highlight the subset of the 1FHL sources that are best candidates for detection at energies above 50-100 GeV with current and future ground-based gamma-ray observatories.
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| 9. |
- Cairns, Marie-Louise, et al.
(författare)
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The potential of electron beam radiation for simultaneous surface modification and bioresorption control of PLLA.
- 2012
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Ingår i: Journal of Biomedical Materials Research. Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 100:9, s. 2223-9
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Tidskriftsartikel (refereegranskat)abstract
- Bioresorbable polymers have been widely investigated as materials exhibiting significant potential for successful application in the fields of tissue engineering and drug delivery. Further to the ability to control degradation, surface engineering of polymers has been highlighted as a key method central to their development. Previous work has demonstrated the ability of electron beam (e-beam) technology to control the degradation profiles and bioresorption of a number of commercially relevant bioresorbable polymers (poly-l-lactic acid (PLLA), L-lactide/DL-lactide co-polymer (PLDL) and poly(lactic-co-glycolic acid (PLGA)). This work investigates the further potential of e-beam technology to impart added biofunctionality through the manipulation of polymer (PLLA) surface properties. PLLA samples were subjected to e-beam treatments in air, with varying beam energies and doses. Surface characterization was then performed using contact angle analysis, X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and atomic force microscopy. Results demonstrated a significant increase in surface wettability post e-beam treatment. In correlation with this, XPS data showed the introduction of oxygen-containing functional groups to the surface of PLLA. Raman spectroscopy indicated chain scission in the near surface region of PLLA (as predicted). However, e-beam effects on surface properties were not shown to be dependent on beam energy or dose. E-beam irradiation did not seem to affect the surface roughness of PLLA as a direct consequence of the treatment.
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| 10. |
- Rademacher, Tim, et al.
(författare)
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Manipulating phloem transport affects wood formation but not local nonstructural carbon reserves in an evergreen conifer
- 2021
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Ingår i: Plant Cell and Environment. - : Wiley. - 0140-7791 .- 1365-3040. ; 44:8, s. 2506-2521
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Tidskriftsartikel (refereegranskat)abstract
- How variations in carbon supply affect wood formation remains poorly understood in particular in mature forest trees. To elucidate how carbon supply affects carbon allocation and wood formation, we attempted to manipulate carbon supply to the cambial region by phloem girdling and compression during the mid- and late-growing season and measured effects on structural development, CO2 efflux and nonstructural carbon reserves in stems of mature white pines. Wood formation and stem CO2 efflux varied with a location relative to treatment (i.e., above or below the restriction). We observed up to twice as many tracheids formed above versus below the treatment after the phloem transport manipulation, whereas the cell-wall area decreased only slightly below the treatments, and cell size did not change relative to the control. Nonstructural carbon reserves in the xylem, needles and roots were largely unaffected by the treatments. Our results suggest that low and high carbon supply affects wood formation, primarily through a strong effect on cell proliferation, and respiration, but local nonstructural carbon concentrations appear to be maintained homeostatically. This contrasts with reports of decoupling of source activity and wood formation at the whole-tree or ecosystem level, highlighting the need to better understand organ-specific responses, within-tree feedbacks, as well as phenological and ontogenetic effects on sink-source dynamics.
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