| 1. |
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| 2. |
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| 3. |
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| 4. |
- Pastorello, A., et al.
(författare)
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ESC and KAIT observations of the transitional type Ia SN 2004eo
- 2007
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Ingår i: Monthly notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 377:4, s. 1531-1552
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Tidskriftsartikel (refereegranskat)abstract
- We present optical and infrared observations of the unusual Type Ia supernova (SN) 2004eo. The light curves and spectra closely resemble those of the prototypical SN 1992A, and the luminosity at maximum (M-B = -19.08) is close to the average for a Type Ia supernova (SN Ia). However, the ejected Ni-56 mass derived by modelling the bolometric light curve (about 0.45M(circle dot)) lies near the lower limit of the Ni-56 mass distribution observed in normal SNe Ia. Accordingly, SN 2004eo shows a relatively rapid post-maximum decline in the light curve [Delta m(15)(B)(true) = 1.46], small expansion velocities in the ejecta and a depth ratio Si II lambda 5972/ Si II lambda 6355 similar to that of SN 1992A. The physical properties of SN 2004eo cause it to fall very close to the boundary between the faint, low-velocity gradient and high-velocity gradient subgroups proposed by Benetti et al. Similar behaviour is seen in a few other SNe Ia. Thus, there may in fact exist a few SNe Ia with intermediate physical properties.
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| 5. |
- Tommasini, R., et al.
(författare)
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Accepted Tutorials at The Web Conference 2022
- 2022
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Ingår i: WWW 2022 - Companion Proceedings of the Web Conference 2022. - New York, NY, USA : Association for Computing Machinery (ACM). ; , s. 391-399
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Konferensbidrag (refereegranskat)abstract
- This paper summarizes the content of the 20 tutorials that have been given at The Web Conference 2022: 85% of these tutorials are lecture style, and 15% of these are hands on.
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| 6. |
- Feng, Shaohong, et al.
(författare)
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Dense sampling of bird diversity increases power of comparative genomics
- 2020
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 587:7833
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Tidskriftsartikel (refereegranskat)abstract
- Whole-genome sequencing projects are increasingly populating the tree of life and characterizing biodiversity(1-4). Sparse taxon sampling has previously been proposed to confound phylogenetic inference(5), and captures only a fraction of the genomic diversity. Here we report a substantial step towards the dense representation of avian phylogenetic and molecular diversity, by analysing 363 genomes from 92.4% of bird families-including 267 newly sequenced genomes produced for phase II of the Bird 10,000 Genomes (B10K) Project. We use this comparative genome dataset in combination with a pipeline that leverages a reference-free whole-genome alignment to identify orthologous regions in greater numbers than has previously been possible and to recognize genomic novelties in particular bird lineages. The densely sampled alignment provides a single-base-pair map of selection, has more than doubled the fraction of bases that are confidently predicted to be under conservation and reveals extensive patterns of weak selection in predominantly non-coding DNA. Our results demonstrate that increasing the diversity of genomes used in comparative studies can reveal more shared and lineage-specific variation, and improve the investigation of genomic characteristics. We anticipate that this genomic resource will offer new perspectives on evolutionary processes in cross-species comparative analyses and assist in efforts to conserve species. A dataset of the genomes of 363 species from the Bird 10,000 Genomes Project shows increased power to detect shared and lineage-specific variation, demonstrating the importance of phylogenetically diverse taxon sampling in whole-genome sequencing.
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| 7. |
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| 8. |
- Liu, Z. G., et al.
(författare)
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Hemizygous variants in protein phosphatase 1 regulatory subunit 3F (PPP1R3F) are associated with a neurodevelopmental disorder characterized by developmental delay, intellectual disability and autistic features
- 2023
-
Ingår i: Human Molecular Genetics. - 0964-6906 .- 1460-2083. ; 32:20, s. 2981-2995
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Tidskriftsartikel (refereegranskat)abstract
- Protein phosphatase 1 regulatory subunit 3F (PPP1R3F) is a member of the glycogen targeting subunits (GTSs), which belong to the large group of regulatory subunits of protein phosphatase 1 (PP1), a major eukaryotic serine/threonine protein phosphatase that regulates diverse cellular processes. Here, we describe the identification of hemizygous variants in PPP1R3F associated with a novel X-linked recessive neurodevelopmental disorder in 13 unrelated individuals. This disorder is characterized by developmental delay, mild intellectual disability, neurobehavioral issues such as autism spectrum disorder, seizures and other neurological findings including tone, gait and cerebellar abnormalities. PPP1R3F variants segregated with disease in affected hemizygous males that inherited the variants from their heterozygous carrier mothers. We show that PPP1R3F is predominantly expressed in brain astrocytes and localizes to the endoplasmic reticulum in cells. Glycogen content in PPP1R3F knockout astrocytoma cells appears to be more sensitive to fluxes in extracellular glucose levels than in wild-type cells, suggesting that PPP1R3F functions in maintaining steady brain glycogen levels under changing glucose conditions. We performed functional studies on nine of the identified variants and observed defects in PP1 binding, protein stability, subcellular localization and regulation of glycogen metabolism in most of them. Collectively, the genetic and molecular data indicate that deleterious variants in PPP1R3F are associated with a new X-linked disorder of glycogen metabolism, highlighting the critical role of GTSs in neurological development. This research expands our understanding of neurodevelopmental disorders and the role of PP1 in brain development and proper function.
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| 9. |
- Prentice, S. J., et al.
(författare)
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SN 2016coi/ASASSN-16fp : an example of residual helium in a type Ic supernova?
- 2018
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Ingår i: Monthly notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 478:3, s. 4162-4192
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Tidskriftsartikel (refereegranskat)abstract
- The optical observations of Ic-4 supernova (SN) 2016coi/ASASSN-16fp, from similar to 2 to similar to 450 d after explosion, are presented along with analysis of its physical properties. The SN shows the broad lines associated with SNe Ic-3/4 but with a key difference. The early spectra display a strong absorption feature at similar to 5400 angstrom which is not seen in other SNe Ic-3/4 at this epoch. This feature has been attributed to He I in the literature. Spectral modelling of the SN in the early photospheric phase suggests the presence of residual He in a C/O dominated shell. However, the behaviour of the He I lines is unusual when compared with He-rich SNe, showing relatively low velocities and weakening rather than strengthening over time. The SN is found to rise to peak similar to 16 d after core-collapse reaching a bolometric luminosity of L-p similar to 3 x 10(42) erg s(-1). Spectral models, including the nebular epoch, show that the SN ejected 2.5-4M(circle dot) of material, with similar to 1.5M(circle dot) below 5000 km s(-1), and with a kinetic energy of (4.5-7) x 10(51) erg. The explosion synthesized similar to 0.14M(circle dot) of Ni-56. There are significant uncertainties in E(B - V)(host) and the distance, however, which will affect L-p and M-Ni. SN 2016coi exploded in a host similar to the Large Magellanic Cloud (LMC) and away from star-forming regions. The properties of the SN and the host-galaxy suggest that the progenitor had M-ZAMS of 23-28M(circle dot) and was stripped almost entirely down to its C/O core at explosion.
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| 10. |
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| 11. |
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| 12. |
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| 13. |
- Walther, G, et al.
(författare)
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Report on challenges for SCIs
- 2021
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This report discusses the challenges posed by four types of threats – terrorist attacks, cyber-attacks, extreme weather and social unrest – on the following eight smart critical infrastructure systems:a.ALPHA - Finance (financial system): The analysis focuses on disturbed information flow and disabling/manipulating IT and communication systems, including attacks on the “physical layer” using the example of IEMI/HPEM threats, as well as the software layer.b. BRAVO - Energy supply (system): The analysis focuses on disruption of “smart” energy supply in a “smart city”, caused by natural hazards, in this case flooding, leading to cascading effects and severe consequences for other energy-depending SCIs.c. CHARLIE - Health care (system):Focus of the analysis is on all threats that might cause large increases in the numbers of injuries or sick patients within a densely populated area. This will include indirect impacts, e.g. large numbers of injuries caused by a disaster or terrorist attacks or disease epidemics, but also direct impacts, e.g. service disruptions in critical health infrastructures, such as hospitals, due to attacks or disasters hitting the infrastructure itself.d.DELTA - Transportation (system) – airports: According to the framework situation, threats on Smart Airports will be assessed under circumstances of (i) blocked traffic, (ii) passenger and airplane traffic exceeding capacity (iii) flood.e. ECHO - Industry (in zones in cities) "Industrial Production Plants":The analysis focuses mainly on technological accidents within the refinery complex, but also accidents caused by natural hazards affecting refinery property outside the main refinery complex, e.g. accident on jetty belonging to refinery on the river Danube during unloading/loading oil products from barge to a tank, damages by a gale or storm on process installations (pipes, hoses) resulting in river pollution. Both scenarios could lead to cascading effects for other SCIs in close vicinity.f. FOXTROT - Water supply (systems): The analysis focuses on three cases of local and regional drinking water supply chains, with different kinds of vulnerabilities in terms of climate threats, ICT challenges, security issues and human error.g.GOLF - Urban flood protection (systems): The analysis focuses in the disruption of water and transport caused through tidal and fluvial flooding events.h. HOTEL: City of Helsinki - Flooding underground coal storage. Resilience of the energy infrastructure (city environment).The way this analysis was conducted was by assessing these threats using a 5x5 framework matrix. The two axes of the matrix were phases (understand risks, anticipate/prepare, absorb/withstand, respond/recover, adapt/learn) and dimensions (system/physical, information/data, organizational/business, societal/political, cognitive/decision-making).Each individual matrix block was discussed by subject experts who identified specific challenges and implications for each matrix element and rated its relevance (high, medium, low).In terms of the results, the system/physical dimension received the highest number of important challenges.Overall, the most important singular element was to understand risks in the organizational/business dimension. The least importance was attributed to the adapt/learn phase.
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| 14. |
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| 15. |
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| 16. |
- Andrighetto, Giulia, et al.
(författare)
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Changes in social norms during the early stages of the COVID-19 pandemic across 43 countries
- 2024
-
Ingår i: Nature Communications. - : NATURE PORTFOLIO. - 2041-1723. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- The emergence of COVID-19 dramatically changed social behavior across societies and contexts. Here we study whether social norms also changed. Specifically, we study this question for cultural tightness (the degree to which societies generally have strong norms), specific social norms (e.g. stealing, hand washing), and norms about enforcement, using survey data from 30,431 respondents in 43 countries recorded before and in the early stages following the emergence of COVID-19. Using variation in disease intensity, we shed light on the mechanisms predicting changes in social norm measures. We find evidence that, after the emergence of the COVID-19 pandemic, hand washing norms increased while tightness and punishing frequency slightly decreased but observe no evidence for a robust change in most other norms. Thus, at least in the short term, our findings suggest that cultures are largely stable to pandemic threats except in those norms, hand washing in this case, that are perceived to be directly relevant to dealing with the collective threat. Tightness-looseness theory predicts that social norms strengthen following threat. Here the authors test this and find that, after the emergence of the COVID-19 pandemic, hand washing norms increased, but no evidence was observed for a robust change in most other norms.
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| 17. |
- Barani-Beiranvand, Hossein, et al.
(författare)
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Phylogeny of penduline tits inferred from mitochondrial and microsatellite genotyping
- 2017
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Ingår i: Journal of Avian Biology. - : WILEY. - 0908-8857 .- 1600-048X. ; 48:7, s. 932-940
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Tidskriftsartikel (refereegranskat)abstract
- Penduline tits (Remiz spp.) are renowned for their diverse mating and parenting strategies, and are a well-studied system by behavioural ecologists. However, the phylogenetic relationships and species delimitations within this genus are poorly understood. Here, we investigate phylogenetic relationships within the genus Remiz by examining the genetic variation in the mitochondrial cytochrome-b gene of 64 individuals and in ten autosomal microsatellite markers from 44 individuals. The taxon sampling includes individuals from all currently recognized species (R. pendulinus, R. macronyx, R. coronatus, and R. consobrinus) and most subspecies in the Palearctic region. We showed that R. coronatus and R. consobrinus are genetically well differentiated and constitute independent evolutionary lineages, separated from each other and from R. pendulinus/macronyx. However, we found no evidence for significant differentiation among R. pendulinus/macronyx individuals in mtDNA haplotypes and only marginal differences between R. pendulinus and R. macronyx in microsatellite markers. Hence, based on present data our recommendation is to treat R. pendulinus and R. macronyx as conspecific and R. coronatus and R. consobrinus as separate species.
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| 18. |
- Cabral, Joao P, et al.
(författare)
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Rapidly Testing the Interaction Model of a Pronunciation Training System via Wizard-of-Oz.
- 2012
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Ingår i: Proceedings of the International Conference on Language Resources and Evaluation. ; , s. 4136-4142
-
Konferensbidrag (refereegranskat)abstract
- This paper describes a prototype of a computer-assisted pronunciation training system called MySpeech. The interface of the MySpeech system is web-based and it currently enables users to practice pronunciation by listening to speech spoken by native speakers and tuning their speech production to correct any mispronunciations detected by the system. This practice exercise is facilitated in different topics and difficulty levels. An experiment was conducted in this work that combines the MySpeech service with the WebWOZ Wizard-of-Oz platform (http://www.webwoz.com), in order to improve the human-computer interaction (HCI) of the service and the feedback that it provides to the user. The employed Wizard-of-Oz method enables a human (who acts as a wizard) to give feedback to the practising user, while the user is not aware that there is another person involved in the communication. This experiment permitted to quickly test an HCI model before its implementation on the MySpeech system. It also allowed to collect input data from the wizard that can be used to improve the proposed model. Another outcome of the experiment was the preliminary evalua- tion of the pronunciation learning service in terms of user satisfaction, which would be difficult to conduct before integrating the HCI part.
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| 19. |
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| 20. |
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| 21. |
- Jing, X, et al.
(författare)
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COVID-19 instigates adipose browning and atrophy through VEGF in small mammals
- 2022
-
Ingår i: Nature metabolism. - : Springer Science and Business Media LLC. - 2522-5812. ; 4:12, s. 1674-
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Tidskriftsartikel (refereegranskat)abstract
- Patients with COVID-19 frequently manifest adipose atrophy, weight loss and cachexia, which significantly contribute to poor quality of life and mortality1,2. Browning of white adipose tissue and activation of brown adipose tissue are effective processes for energy expenditure3–7; however, mechanistic and functional links between SARS-CoV-2 infection and adipose thermogenesis have not been studied. In this study, we provide experimental evidence that SARS-CoV-2 infection augments adipose browning and non-shivering thermogenesis (NST), which contributes to adipose atrophy and body weight loss. In mouse and hamster models, SARS-CoV-2 infection activates brown adipose tissue and instigates a browning or beige phenotype of white adipose tissues, including augmented NST. This browning phenotype was also observed in post-mortem adipose tissue of four patients who died of COVID-19. Mechanistically, high levels of vascular endothelial growth factor (VEGF) in the adipose tissue induces adipose browning through vasculature–adipocyte interaction. Inhibition of VEGF blocks COVID-19-induced adipose tissue browning and NST and partially prevents infection-induced body weight loss. Our data suggest that the browning of adipose tissues induced by COVID-19 can contribute to adipose tissue atrophy and weight loss observed during infection. Inhibition of VEGF signaling may represent an effective approach for preventing and treating COVID-19-associated weight loss.
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| 22. |
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| 23. |
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| 24. |
- Máthé, I., et al.
(författare)
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Investigation of mineral water springs of Miercurea Ciuc (Csíkszereda) region (Romania) with cultivation-dependent microbiological methods
- 2010
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Ingår i: Acta Microbiologica et Immunologica Hungarica. - 1217-8950 .- 1588-2640. ; 57:2, s. 109-122
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Tidskriftsartikel (refereegranskat)abstract
- Water samples of ten mineral water springs at Miercurea Ciuc (Csíkszereda) region (Romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. The results of standard hygienic bacteriological tests showed that the Hargita Spring had perfect and five other springs had microbiologically acceptable water quality (Zsögöd-, Nagy-borvíz-, Taploca-, Szentegyháza- and Lobogó springs). The water of Borsáros Spring was exceptionable (high germ count, presence of Enterococcus spp.).Both standard bacteriological and molecular microbiological methods indicated that the microbiological water quality of the Szeltersz-, Nádasszék- and Délo springs was not acceptable. Bad water quality resulted from inadequate spring catchment and hygiene (low yield, lack of runoff, negligent usage of the springs, horse manure around the spring).The 16S rRNA gene-based identification of strains isolated on standard meat-peptone medium resulted in the detection of typical aquatic organisms such as Shewanella baltica, Aeromonas spp., Pseudomonas veronii, Psychrobacter sp,. Acinetobacter spp. and allochthonous microbes, like Nocardia, Streptomyces, Bacillus, Microbacterium , and Arthrobacter strains indicating the impact of soil. Other allochthonous microbes, such as Staphylococcus spp., Micrococcus sp., Lactococcus sp., Clostridium butyricum, Yersinia spp., Aerococcus sp., may have originated from animal/human sources. © 2010 Akadémiai Kiadó, Budapest.
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| 25. |
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| 26. |
- Novák, D., et al.
(författare)
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In flight SEU tests on the European SMART-1 spacecraft
- 2005
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Ingår i: Proceedings of the European Conference on Radiation and its Effects on Components and Systems, RADECS. - 0780395018 - 9780780395015 ; , s. PH11-PH13
-
Konferensbidrag (refereegranskat)abstract
- The SMART-I satellite launched by the European Space Agency has traveled to an orbit around the Moon driven by its own ion engine. Because of the characteristics of the ion engine mission required many revolutions to reach the Moon and the time to pass through the radiation belts around the Earth was significant. The spacecraft is exposed to solar protons throughout its mission. In advance the flight in-beam test data was collected to asses the radiation hardness properties of an SRAM, one of the components of the system unit of the satellite. Single Event Upset data is available for this component and Solar Proton Flux was measured by the GOES satellite in Geosynchronous orbit. The aim of this work is to compare the predictions of the in-beam tests and the effects of the in space irradiation of SRAM circuits on board.
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| 27. |
- Pablos, Isabel, et al.
(författare)
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Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CL(pro) substrate degradome
- 2021
-
Ingår i: Cell Reports. - : Cell Press. - 2211-1247. ; 37:4
-
Tidskriftsartikel (refereegranskat)abstract
- The main viral protease (3CL(pro)) is indispensable for SARS-CoV-2 replication. We delineate the human protein substrate landscape of 3CL(pro) by TAILS substrate-targeted N-terminomics. We identify more than 100 substrates in human lung and kidney cells supported by analyses of SARS-CoV-2-infected cells. Enzyme kinetics and molecular docking simulations of 3CL(pro) engaging substrates reveal how noncanonical cleavage sites, which diverge from SARS-CoV, guide substrate specificity. Cleaving the interactors of essential effector proteins, effectively stranding them from their binding partners, amplifies the consequences of proteolysis. We show that 3CL(pro) targets the Hippo pathway, including inactivation of MAP4K5, and key effectors of transcription, mRNA processing, and translation. We demonstrate that Spike glycoprotein directly binds galectin-8, with galectin-8 cleavage disengaging CALCOCO2/NDP52 to decouple antiviral-autophagy. Indeed, in post-mortem COVID-19 lung samples, NDP52 rarely colocalizes with galectin-8, unlike in healthy lungs. The 3CL(pro) substrate degradome establishes a foundational substrate atlas to accelerate exploration of SARSCoV-2 pathology and drug design.
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| 28. |
- Pasin, G. L., et al.
(författare)
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Evidence from 43 countries that disease leaves cultures unchanged in the short-term
- 2024
-
Ingår i: Scientific Reports. - : Nature Research. - 2045-2322. ; 14:1
-
Tidskriftsartikel (refereegranskat)abstract
- Did cultures change shortly after, and in response to, the COVID-19 outbreak? If so, then in what way? We study these questions for a set of macro-cultural dimensions: collectivism/individualism, duty/joy, traditionalism/autonomy, and pro-fertility/individual-choice norms. We also study specific perceptions and norms like perceived threats to society (e.g. immigration) and hygiene norms. We draw on Evolutionary Modernization Theory, Parasite Stress Theory, and the Behavioural Immune System, and existing evidence, to make an overarching prediction: the COVID-19 pandemic should increase collectivism, duty, traditionalism, conformity (i.e. pro-fertility), and outgroup prejudice. We derive specific hypotheses from this prediction and use survey data from 29,761 respondents, in 55 cities and 43 countries, collected before (April–December 2019) and recently after the emergence of COVID-19 (April–June 2020) to test them. We exploit variation in disease intensity across regions to test potential mechanisms behind any changes. The macro-cultural dimensions remained stable. In contrast, specific perceptions and norms related to the pandemic changed: norms of hygiene substantially increased as did perceived threats related to disease. Taken together, our findings imply that macro-cultural dimensions are primarily stable while specific perceptions and norms, particularly those related to the pandemic, can change rapidly. Our findings provide new evidence for theories of cultural change and have implications for policy, public health, daily life, and future trajectories of our societies.
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| 29. |
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| 30. |
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| 31. |
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| 32. |
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| 33. |
- Székely, Éva, et al.
(författare)
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Clustering Expressive Speech Styles in Audiobooks Using Glottal Source Parameters.
- 2011
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Ingår i: 12th Annual Conference of the International-Speech-Communication-Association 2011 (INTERSPEECH 2011). - : ISCA-INT SPEECH COMMUNICATION ASSOC. - 9781618392701 ; , s. 2409-2412
-
Konferensbidrag (refereegranskat)abstract
- A great challenge for text-to-speech synthesis is to produce ex- pressive speech. The main problem is that it is difficult to syn- thesise high-quality speech using expressive corpora. With the increasing interest in audiobook corpora for speech synthesis, there is a demand to synthesise speech which is rich in prosody, emotions and voice styles. In this work, Self-Organising Fea- ture Maps (SOFM) are used for clustering the speech data using voice quality parameters of the glottal source, in order to map out the variety of voice styles in the corpus. Subjective evalu- ation showed that this clustering method successfully separated the speech data into groups of utterances associated with dif- ferent voice characteristics. This work can be applied in unit- selection synthesis by selecting appropriate data sets to synthe- sise utterances with specific voice styles. It can also be used in parametric speech synthesis to model different voice styles separately.
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| 34. |
- Székely, Éva, et al.
(författare)
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Evaluating expressive speech synthesis from audiobooks in conversational phrases
- 2012
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Konferensbidrag (refereegranskat)abstract
- Audiobooks are a rich resource of large quantities of natural sounding, highly expressive speech. In our previous research we have shown that it is possible to detect different expressive voice styles represented in a particular audiobook, using unsupervised clustering to group the speech corpus of the audiobook into smaller subsets representing the detected voice styles. These subsets of corpora of different voice styles reflect the various ways a speaker uses their voice to express involvement and affect, or imitate characters. This study is an evaluation of the detection of voice styles in an audiobook in the application of expressive speech synthesis. A further aim of this study is to investigate the usability of audiobooks as a language resource for expressive speech synthesis of utterances of conversational speech. Two evaluations have been carried out to assess the effect of the genre transfer: transmitting expressive speech from read aloud literature to conversational phrases with the application of speech synthesis. The first evaluation revealed that listeners have different voice style preferences for a particular conversational phrase. The second evaluation showed that it is possible for users of speech synthesis systems to learn the characteristics of a certain voice style well enough to make reliable predictions about what a certain utterance will sound like when synthesised using that voice style.
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| 35. |
- Székely, Éva, et al.
(författare)
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Predicting synthetic voice style from facial expressions. An application for augmented conversations
- 2014
-
Ingår i: Speech Communication. - : Elsevier BV. - 0167-6393 .- 1872-7182. ; 57, s. 63-75
-
Tidskriftsartikel (refereegranskat)abstract
- The ability to efficiently facilitate social interaction and emotional expression is an important, yet unmet requirement for speech generating devices aimed at individuals with speech impairment. Using gestures such as facial expressions to control aspects of expressive synthetic speech could contribute to an improved communication experience for both the user of the device and the conversation partner. For this purpose, a mapping model between facial expressions and speech is needed, that is high level (utterance-based), versatile and personalisable. In the mapping developed in this work, visual and auditory modalities are connected based on the intended emotional salience of a message: the intensity of facial expressions of the user to the emotional intensity of the synthetic speech. The mapping model has been implemented in a system called WinkTalk that uses estimated facial expression categories and their intensity values to automat- ically select between three expressive synthetic voices reflecting three degrees of emotional intensity. An evaluation is conducted through an interactive experiment using simulated augmented conversations. The results have shown that automatic control of synthetic speech through facial expressions is fast, non-intrusive, sufficiently accurate and supports the user to feel more involved in the conversation. It can be concluded that the system has the potential to facilitate a more efficient communication process between user and listener.
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| 36. |
- Székely, Éva, et al.
(författare)
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WinkTalk : a demonstration of a multimodal speech synthesis platform linking facial expressions to expressive synthetic voices
- 2012
-
Ingår i: Proceedings of the Third Workshop on Speech and Language Processing for Assistive Technologies. - : Association for Computational Linguistics. ; , s. 5-8
-
Konferensbidrag (refereegranskat)abstract
- This paper describes a demonstration of the WinkTalk system, which is a speech synthe- sis platform using expressive synthetic voices. With the help of a webcamera and facial ex- pression analysis, the system allows the user to control the expressive features of the syn- thetic speech for a particular utterance with their facial expressions. Based on a person- alised mapping between three expressive syn- thetic voices and the users facial expressions, the system selects a voice that matches their face at the moment of sending a message. The WinkTalk system is an early research pro- totype that aims to demonstrate that facial expressions can be used as a more intuitive control over expressive speech synthesis than manual selection of voice types, thereby con- tributing to an improved communication expe- rience for users of speech generating devices.
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| 37. |
- Székely, Éva, et al.
(författare)
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WinkTalk: a multimodal speech synthesis interface linking facial expressions to expressive synthetic voices
- 2012
-
Ingår i: Proceedings of the Third Workshop on Speech and Language Processing for Assistive Technologies.
-
Konferensbidrag (refereegranskat)abstract
- This paper describes a demonstration of the WinkTalk system, which is a speech synthe- sis platform using expressive synthetic voices. With the help of a webcamera and facial ex- pression analysis, the system allows the user to control the expressive features of the syn- thetic speech for a particular utterance with their facial expressions. Based on a person- alised mapping between three expressive syn- thetic voices and the users facial expressions, the system selects a voice that matches their face at the moment of sending a message. The WinkTalk system is an early research pro- totype that aims to demonstrate that facial expressions can be used as a more intuitive control over expressive speech synthesis than manual selection of voice types, thereby con- tributing to an improved communication expe- rience for users of speech generating devices.
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| 38. |
- Szekely, L, et al.
(författare)
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Human herpesvirus-8-encoded LNA-1 accumulates in heterochromatin- associated nuclear bodies
- 1999
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Ingår i: The Journal of general virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 8080 ( Pt 11), s. 2889-2900
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Tidskriftsartikel (refereegranskat)abstract
- Subnuclear distribution of the human herpesvirus-8 (HHV-8)- encoded nuclear protein LNA-1 was analysed at high resolution in body cavity (BC) lymphoma-derived cell lines, in cell hybrids between BC cells and various human and mouse cells and in freshly infected K562 and ECV cell lines. Three-dimensional reconstruction of nuclei from optical sections and quantitative analysis of the distribution of LNA-1 fluorescence in relation to chromatin showed that LNA-1 associates preferentially with the border of heterochromatin in the interphase nuclei. This was further confirmed in the following systems: in endo- and exonuclease-digested nuclei, in human–mouse (BC-1–Sp2- 0) hybrids and on chromatin spreads. LNA-1 was found to bind to mitotic chromosomes at random. Epstein–Barr virus (EBV), but not HHV-8, was rapidly lost from mouse–human hybrid cells in parallel with the loss of human chromosomes. HHV-8 could persist on the residual mouse background for more than 8 months. In early human–mouse hybrids that contain a single fused nucleus, LNA-1 preferentially associates with human chromatin. After the gradual loss of the human chromosomes, LNA-1 becomes associated with the murine pericentromeric heterochromatin. In human–human hybrids derived from the fusion of the HHV-8-carrying BCBL-1 cells and the EBV-immortalized lymphoblastoid cell line IB4, LNA-1 did not co-localize with EBNA-1, EBNA-2, EBNA-5 or EBNA-6. LNA-1 was not associated with PML containing ND10 bodies either. DNase but not RNase or detergent treatment of isolated nuclei destroys LNA-1 bodies. In advanced apoptotic cells LNA- 1 bodies remain intact but are not included in the apoptotic bodies themselves.
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- Tuerkova, Alzbeta, et al.
(författare)
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Identifying Novel Inhibitors for Hepatic Organic Anion Transporting Polypeptides by Machine Learning-Based Virtual Screening
- 2022
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Ingår i: Journal of Chemical Information and Modeling. - : American Chemical Society (ACS). - 1549-9596 .- 1549-960X. ; 62:24, s. 6323-6335
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Tidskriftsartikel (refereegranskat)abstract
- Integration of statistical learning methods with structure-based modeling approaches is a contemporary strategy to identify novel lead compounds in drug discovery. Hepatic organic anion transporting polypeptides (OATP1B1, OATP1B3, and OATP2B1) are classical off-targets, and it is well recognized that their ability to interfere with a wide range of chemically unrelated drugs, environmental chemicals, or food additives can lead to unwanted adverse effects like liver toxicity and drug-drug or drug-food interactions. Therefore, the identification of novel (tool) compounds for hepatic OATPs by virtual screening approaches and subsequent experimental validation is a major asset for elucidating structure-function relationships of (related) transporters: they enhance our understanding about molecular determinants and structural aspects of hepatic OATPs driving ligand binding and selectivity. In the present study, we performed a consensus virtual screening approach by using different types of machine learning models (proteochemometric models, conformal prediction models, and XGBoost models for hepatic OATPs), followed by molecular docking of preselected hits using previously established structural models for hepatic OATPs. Screening the diverse REAL drug-like set (Enamine) shows a comparable hit rate for OATP1B1 (36% actives) and OATP1B3 (32% actives), while the hit rate for OATP2B1 was even higher (66% actives). Percentage inhibition values for 44 selected compounds were determined using dedicated in vitro assays and guided the prioritization of several highly potent novel hepatic OATP inhibitors: six (strong) OATP2B1 inhibitors (IC50 values ranging from 0.04 to 6 μM), three OATP1B1 inhibitors (2.69 to 10 μM), and five OATP1B3 inhibitors (1.53 to 10 μM) were identified. Strikingly, two novel OATP2B1 inhibitors were uncovered (C7 and H5) which show high affinity (IC50 values: 40 nM and 390 nM) comparable to the recently described estrone-based inhibitor (IC50 = 41 nM). A molecularly detailed explanation for the observed differences in ligand binding to the three transporters is given by means of structural comparison of the detected binding sites and docking poses.
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