| 1. |
|
|
| 2. |
- Arwin, Hans, 1950-, et al.
(författare)
-
Infrared ellipsometry studies of thermal stability of protein monolayers and multilayers
- 2008
-
Ingår i: Physica Status Solidi. C: Current Topics in Solid State Physics. - Weinheim, Germany : Wiley-VCH Verlagsgesellschaft. - 1862-6351. ; 5:5, s. 1438-1441
-
Tidskriftsartikel (refereegranskat)abstract
- Methodology for studies of effects of heating multilayers of human serum albumin (HSA) and anti-HSA is presented. Multilayers of anti-HSA were prepared on silicon substrates and studied with infrared spectroscopic ellipsometry equipped with a heat stage. The refractive index N = n + ik and the layer thickness are determined and the amide bands are analyzed. It is found that HSA/anti-HSA multilayers are stable for shorter times at temperatures above 100 °C, except for small thickness changes. Also pilot studies of effects of heating monolayers of proteins adsorbed on gold substrates is presented.
|
|
| 3. |
- Berg, Cecilia, 1976-, et al.
(författare)
-
Platelet-induced growth of human fibroblasts is associated with an increased expression of 5-lipoxygenase
- 2006
-
Ingår i: Thrombosis and Haemostasis. - 0340-6245 .- 2567-689X. ; 96:5, s. 652-659
-
Tidskriftsartikel (refereegranskat)abstract
- Proliferation of fibroblasts is vital for adequate wound healing but is probably also involved in different hyperproliferative disorders such as atherosclerosis and cancer. The regeneration of tissue usually starts with coagulation, involving release of mitogenic and inflammatory factors from activated platelets. This study focuses on the role of eicosanoids in the proliferative effects of platelets on human fibroblasts. We show that the phospholipase A2 inhibitor 7,7-dimethyl-5,8-eicosadienoic acid (DMDA), the combined cyclooxygenase (COX) and lipoxygenase (LOX) inhibitor 5,8,11,14-eicosatetraynoic acid (ETYA) and the LOX inhibitor 5,8,11-eicosatriynoic acid (ETI) block the platelet-induced proliferation of serum starved subconfluent human fibroblasts. Anti-proliferative effects were also obtained by specific inhibition of 5-LOX with 5,6-dehydro arachidonic acid (5,6-dAA), whereas the 12-LOX inhibitor cinnamyl-3,4-dihydroxy-α-cyanocinnamate (CDC) did not affect the platelet-stimulated growth of fibroblasts. The expression of 5-LOX was analyzed by reverse-transcriptase-mediated PCR (RT-PCR), Western blotting and HPLC. 5-LOX message and protein was detected in fibroblasts but not in platelets. Incubation with platelets markedly increased, already after one hour, the expression of 5-LOX in the fibroblast culture. The increased 5-LOX activity was associated with an elevated level of the 5-LOX metabolite 5-hydroxyeicosatetraenoic acid (5-HETE) reaching its maximum after 1-2 hours of co-incubation of fibroblasts and platelets. The 5-HETE production was reduced by the inhibitors DMDA, ETYA and ETI. In conclusion, this study suggests that platelet-stimulated proliferation of fibroblasts is mediated by an increased 5-LOX activity, which supports recent findings indicating a crucial role for this enzyme in proliferative disorders such as atherosclerosis. © 2006 Schattauer GmbH, Stuttgart.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Brunette, Donald M, et al.
(författare)
-
Titanium in Medicine : material science, surface science, engineering, biological responses and medical applications
- 2001
-
Bok (övrigt vetenskapligt/konstnärligt)abstract
- This comprehensive book provides state-of-the-art scientific and technical information in a clear format and consistent structure making it suitable for formal course work or self-instruction. The authors are drawn not only from academic institutions but also from industry, so that practical aspects of implant fabrication and material handling are covered that are often lacking in biomaterials texts. Besides readers with a general interest in biomaterials, the book will interest materials investigators, surgeons and dentists using titanium implants, medical scientists and engineers, as well as lecturers at universities or institutes who would benefit by having ready access to authoritative information on the use of titanium for implants, devices and instruments. More information: http://www.titaniuminmedicine.com.
|
|
| 8. |
- Hansson, Kenny, 1972-, et al.
(författare)
-
Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay
- 1999
-
Ingår i: Biosensors & bioelectronics. - 0956-5663 .- 1873-4235. ; 14:8-9, s. 671-682
-
Tidskriftsartikel (refereegranskat)abstract
- It is previously shown that surface plasmon resonance (SPR) can be used to study blood plasma coagulation. This work explores the use of this technique for the analysis of tissue factor induced coagulation, i.e. prothrombin time (PT) analysis, of whole blood and plasma. The reference method was nephelometry. The prothrombin time analysis by SPR was performed by mixing two volumes of blood/plasma, one volume of thromboplastin, and one volume of CaCl2 solution directly on a sensor surface. The measurements show good agreement between nephelometry and SPR plasma analysis and also between SPR plasma and whole blood analysis. The effect of anticoagulant treatment on the clotting times was significant both quantitatively and qualitatively. The impact on the SPR signal of different physiological events in the coagulation process is discussed, and tentative interpretations of the sensorgram features are given. The major advantage of the SPR method compared to nephelometry is the possibility to perform analysis on whole blood instead of plasma. In conclusion, SPR is a promising method for whole blood coagulation analysis.
|
|
| 9. |
- Källtorp, Mia, et al.
(författare)
-
In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces.
- 1999
-
Ingår i: Biomaterials. - 0142-9612. ; 20:22, s. 2123-37
-
Tidskriftsartikel (refereegranskat)abstract
- Hydroxylated and methylated surfaces were prepared by the self-assembled monolayer technique (SAM) of alkane thiols on gold. The surfaces were used to evaluate the influence of implant surface chemistry on protein deposition and inflammatory cell response. Implants were inserted subcutaneously in the rat for 3 and 24 h. The surface chemical properties influenced the in vitro rat plasma protein adsorption (ellipsometry/antibody) with few exceptions (albumin not found and fibrinogen always found). The number of recruited cells and their distribution (DNA from implant versus from exudate) was influenced by the different chemistries at 24 h, but not at 3 h. HIS48+, ED1+, ED2+ and small numbers of CD5+ cells were present in the exudate at both time periods (flow cytometry). The cellular oxidative metabolism was low, although cells on -OH surfaces responded with the highest phorbol ester-stimulated chemiluminescence (CL)/DNA. The levels of cytokines IL-1alpha, IL-1beta and TNFalpha (ELISA) were not influenced by material surface chemistry. Sham operated sites had a higher cytokine concentration/DNA compared with exudates from an implant milieu. The results of this study show that surface chemical functionalization modifies specific events in the inflammatory response around implants in soft tissues.
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
- Skoglund, Caroline, 1981-, et al.
(författare)
-
C-reactive protein inhibit complement-mediated platelet activation suggesting a protective role in atherogenesis
- 2006
-
Ingår i: Atherosclerosis Supplements. - Clare, Ireland : Elsevier. - 1567-5688 .- 1878-5050. ; 7:3, s. 284-284
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Objective: C-reactive protein (CRP) represents a powerful predictor of coro- nary artery disease. However, its physiological role is not fully understood. The binding of CRP to its ligand phosphorylcholine (PC) activates the com- plement system via the classical pathway, although limited to the initial stages, i.e. no membrane attack complex is formed. The aim of this study was to chaxacterize CRP-induced complement activation on PC-coated surfaces, and to investigate the regulatory effects of PC-bound crp on complement induced platelet activation.Methods: PC conjugated to keyhole limpet hemocyanin was immobilized to cross-linked fibrinogen on silica particles. Ellipsometry and polyclonal anti- bodies were used to quantify deposition of serum proteins, complement factors and CRP on the surfaces. Washed platelets as well as serum were prepared according to standard protocols. CRP concentrations were measured with a high sensitivity assay. Lumi-aggregometry was used to evaluate the effects of PC-coated particles and CRP on complement-induced platelet aggregation and secretion.Results: Serum (5%) induced platelet aggregation and secretion through complement-dependent mechanisms. PC-coated particles antagonized the complement-mediated platelet activation but only if CRP was present. Inter- estingly, we found that a minor elevation of CRR below 5 rag/1 was sufficient to inhibit platelet activation.Conclusions: We suggest that CRP bound to PC-expressing ligands, e.g. bacteria or modified low-density lipoproteins in an atherosclerotic lesion, modulate complement activation and thereby prevent a harmful platelet activation.
|
|
| 13. |
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
- Tengvall, Pentti, 1951-
(författare)
-
How Surfaces Interact with the Biological Environment
- 2003
-
Ingår i: Bio-Implant Interface, Improving Biomaterials and Tissue Reactions. - Boca Raton, Florida : CRC Press LLC. - 0849314747 - 9780849314742 ; , s. 285-303
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Bringing together a group of scientists, this book describes interactions at the interface between biomaterials and living tissue. It focuses is on the importance of the implant surface's topography and chemistry to its interaction with the biological environment. It includes a section that explores the potential of advances of research.
|
|
| 17. |
- Tengvall, Pentti, 1951-, et al.
(författare)
-
Surface Properties and Osseointegration
- 2005. - 1
-
Ingår i: The Osseointegration Book; from Calvarium to Calcaneus. - Berlin : Quintessenz VerlagsGmbH. - 1850970904 - 9781850970903 ; , s. -494
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Branemark Osseointegration Center, Goteborg, Sweden. Comprehensive reference brings together the background and history of the subject and covers osseointegration from its origins, through theory to practice. Presents a wealth of data about the current applications and covers its origins in dentistry to facial reconstruction and orthopedics. High-quality color images.
|
|
| 18. |
|
|
