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Träfflista för sökning "WFRF:(Netzel Elvy) srt2:(1979)"

Search: WFRF:(Netzel Elvy) > (1979)

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  • Humble, Elisabet, et al. (author)
  • Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide
  • 1979
  • In: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 90:3, s. 1064-1072
  • Journal article (peer-reviewed)abstract
    • Rat liver fructose-1,6-diphosphatase was phosphorylated with (32P)ATP and the catalytic subunit of cyclic AMP-dependent protein kinase from pig muscle. After digestion with pepsin, α-chymotrypsin and subtilisin a peptide with the amino-terminal sequence Ser-Arg-Tyr-(32P)SerP-Leu-Pro-Leu-Pro was isolated. A synthetic unphosphorylated heptapeptide with the same amino acid sequence, ending with leucine, was phosphorylated with an apparent Km of 400 μM, while the apparent Km value for fructose-1,6-diphosphatase was 30 μM (subunit concentration). The Vmax value was 20 times higher for the peptide than for the enzyme.
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  • Result 1-1 of 1
Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Ek, Pia (1)
Humble, Elisabet (1)
Engström, Lorentz (1)
Dahlqvist-Edberg, Ul ... (1)
Ragnarsson, Ulf (1)
Netzel, Elvy (1)
University
Uppsala University (1)
Language
English (1)
Research subject (UKÄ/SCB)
Medical and Health Sciences (1)
Year
1979 (1)Undo refine

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