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| 13. |
- Chen, Doris, et al.
(författare)
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High-resolution, high-throughput SNP mapping in Drosophila melanogaster
- 2008
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Ingår i: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 5:4, s. 323-329
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Tidskriftsartikel (refereegranskat)abstract
- Single nucleotide polymorphisms (SNPs) are useful markers for genetic mapping experiments in model organisms. Here we report the establishment of a high-density SNP map and high-throughput genotyping assays for Drosophila melanogaster. Our map comprises 27,367 SNPs in common laboratory Drosophila stocks. These SNPs were clustered within 2,238 amplifiable markers at an average density of 1 marker every 50.3 kb, or 6.3 genes. We have also constructed a set of 62 Drosophila stocks, each of which facilitates the generation of recombinants within a defined genetic interval of 1-2 Mb. For flexible, high-throughput SNP genotyping, we used fluorescent tag-array mini-sequencing (TAMS) assays. We designed and validated TAMS assays for 293 SNPs at an average resolution of 391.3 kb, and demonstrated the utility of these tools by rapidly mapping 14 mutations that disrupt embryonic muscle patterning. These resources enable high-resolution high-throughput genetic mapping in Drosophila.
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| 15. |
- Chen, Deliang, 1961, et al.
(författare)
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Using statistical downscaling to quantify the GCM-related uncertainty in regional climate change scenarios: A case study of Swedish precipitation
- 2006
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Ingår i: Advances in Atmospheric Sciences. - 0256-1530. ; 23:1, s. 54-60
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Tidskriftsartikel (refereegranskat)abstract
- There are a number of sources of uncertainty in regional climate change scenarios. When statistical downscaling is used to obtain regional climate change scenarios, the uncertainty may originate from the uncertainties in the global climate models used, the skill of the statistical model, and the forcing scenarios applied to the global climate model. The uncertainty associated with global climate models can be evaluated by examining the differences in the predictors and in the downscaled climate change scenarios based on a set of different global climate models. When standardized global climate model simulations such as the second phase of the Coupled Model Intercomparison Project (CMIP2) are used, the difference in the downscaled variables mainly reflects differences in the climate models and the natural variability in the simulated climates. It is proposed that the spread of the estimates can be taken as a measure of the uncertainty associated with global climate models. The proposed method is applied to the estimation of global-climate-model-related uncertainty in regional precipitation change scenarios in Sweden. Results from statistical downscaling based on 17 global climate models show that there is an overall increase in annual precipitation all over Sweden although a considerable spread of the changes in the precipitation exists. The general increase can be attributed to the increased large-scale precipitation and the enhanced westerly wind. The estimated uncertainty is nearly independent of region. However, there is a seasonal dependence. The estimates for winter show the highest level of confidence, while the estimates for summer show the least.
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| 16. |
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| 17. |
- Finne Wistrand, Anna, et al.
(författare)
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Resorbable Scaffolds from Three Different Techniques : Electrospun Fabrics, Salt-Leaching Porous Films, and Smooth Flat Surfaces
- 2008
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Ingår i: Macromolecular Bioscience. - : Wiley. - 1616-5187 .- 1616-5195. ; 8:10, s. 951-959
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Tidskriftsartikel (refereegranskat)abstract
- Nanofibrous scaffolds of poly[(L-lactide)-co-(1,5-dioxepan-2-one)] generated by electrospinning have been compared with porous films obtained by solvent cast/salt leaching and homogeneous films. A comparison between the fibrous materials and the homogeneous solvent-cast films revelead that the surface of the nanofibers was more hydrophobic and that the nanofibers were degraded more rapidly in the presence of proteinase. It was obvious that the strain-to-break was reduced by the nanofiber formation, it decreased from 370% to 130% independent of fiber diameter. These values were however considerably higher than the strain-to-break of the solvent-cast/salt leaching scaffold. In addition, the nanofibrous material accelerated the adhesion and growth of the mesenchymal stem cell compared to the smooth material.
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| 18. |
- Hanssen-Bauer, I., et al.
(författare)
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Statistical downscaling of climate scenarios over Scandinavia
- 2005
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Ingår i: Climate Research. - : Inter-Research Science Center. - 0936-577X .- 1616-1572. ; 29:3, s. 255-268
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Tidskriftsartikel (refereegranskat)abstract
- Studies from recent years involving development and application of statistical downscaling models for Scandinavia (mainly Norway and Sweden) are reviewed. In most of the studies linear techniques were applied. Local temperature and/or precipitation were predictands in a majority of the studies. Large-scale temperature fields, either from 2 m or 850 hPa, were found to be the best predictors for local temperature, while a combination of atmospheric circulation indices and tropospheric humidity information were the best predictors for local precipitation. Statistically downscaled temperature scenarios for Scandinavia differ depending on climate model, emission scenario and downscaling strategy. There are nevertheless several common features in the temperature scenarios. The warming rates during the 21st century are projected to increase with distance from the coast and with latitude. In most of Scandinavia higher warming rates are projected in winter than in summer. For precipitation, the spread between different scenarios is larger than for temperature. A substantial part of the projected precipitation change is connected to projected changes in atmospheric circulation, which differ considerably from one model integration to another. A tendency for increased large-scale humidity over Scandinavia still implies that projections for the 21st century typically indicate increased annual precipitation. This tendency is most significant during winter. In northern Scandinavia the projections tend to show increased precipitation also during summer, but several scenarios show reduced summer precipitation in parts of southern Scandinavia. Comparisons with results from global and regional climate models indicate that both regional modeling and statistical downscaling add value to the results from the global models.
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