| 1. |
- Christakopoulos, Paul, et al.
(författare)
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Functional characterization of a cellulose binding xylanase from Fusarium oxysporum
- 1996
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Ingår i: Biotechnology letters. - 0141-5492 .- 1573-6776. ; 18:3, s. 349-354
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Tidskriftsartikel (refereegranskat)abstract
- An extracellular endoxylanase from Fusarium oxysporum binds onto crystalline cellulose. A small peptide (~ 2kDa) could be isolated after partial proteolysis of the native protein. It consists of 18 amino acids, is located in the C-terminal region of the protein and corresponds functionally to a cellulose binding domain (CBD), the first one to be reported in a fungal xylanase. The amino acid sequence of this peptide shows no homology with any known CBD
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| 2. |
- Miller, T. C., et al.
(författare)
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Particle astrophysics in antarctica
- 1996
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Ingår i: International School of Cosmic Ray Astrophysics: 10th Course: Toward the Millennium in Astrophysics: Problems and Prospects 16-26 Jun 1996. Erice, Italy. ; , s. 157-166
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Konferensbidrag (refereegranskat)
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| 3. |
- Atac, A, et al.
(författare)
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Single-step link of the superdeformed band in Eu-143
- 1996
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Ingår i: ZEITSCHRIFT FUR PHYSIK A-HADRONS AND NUCLEI. - : SPRINGER VERLAG. - 0939-7922. ; 355:4, s. 343-344
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A discrete gamma-ray transition with an energy of 3360.6 keV deexciting the second lowest SD state in Eu-143 has been discovered. It carries 3.2 % of the full intensity of the band and feeds into a nearly spherical state which is above the I = 35/2((+)),
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| 4. |
- Christakopoulos, Paul, et al.
(författare)
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Production and partial characterization of xylanase from Fusarium oxysporum
- 1996
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Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 58:2, s. 115-119
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Tidskriftsartikel (refereegranskat)abstract
- Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major, one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively
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| 5. |
- Christakopoulos, Paul, et al.
(författare)
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Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3
- 1996
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Ingår i: Journal of Biotechnology. - : Elsevier BV. - 0168-1656 .- 1873-4863. ; 51:2, s. 181-189
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Tidskriftsartikel (refereegranskat)abstract
- Two low molecular mass endo-1,4-β-d-xylanases from Fusarium oxysporum were purified to homogeneity by gel-filtration and ion-exchange chromatography. They exhibit molecular masses of 20.8 (xylanase I) and 23.5 (xylanase II) kDa, and isoelectric points of 9.5 and 8.45–8.70, respectively. Both xylanases display remarkable pH (9.0) stability. At 40 to 55 °C xylanase II is more thermostable than xylanase I but less active on xylan. In contrast to xylanase I, xylanase II is able to hydrolyze 1-O-4-methylumbelliferyl-(β-d-glucopyranosyl)-β-d-xylopyranoside (muxg). Neither of these enzymes hydrolyze xylotriose. They bind on crystalline cellulose but not on insoluble xylan. Analysis of reaction mixtures by high pressure liquid chromatography revealed that both enzymes cleave preferentially the internal glycosidic bonds of xylopentaose and oat spelts xylan. Thus the purified enzymes appeared to be true endo-β-1,4-xylanases. The amino terminal sequences of xylanases I and II show no homology. Xylanase I shows high similarity with alkaline low molecular mass xylanases of family G/11.
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| 6. |
- Demirel, B., et al.
(författare)
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Analyses of products from autoclave reactions : derivations of reaction parameters
- 1996
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Ingår i: Preprints of papers presented - American Chemical Society. Division of Fuel Chemistry. - 0569-3772. ; 41:4, s. 1157-1160
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Tidskriftsartikel (refereegranskat)abstract
- This work presents energetics for high pressure hydroprocessing reactions derived from post analyses of liquid and gaseous products. Specifically, GC and GC/MS were used to follow the product distribution fram the hydrocracking of methyldecalins over zeohte supported palladium and platinum catalysts as a function of temperature. Plain Arrhenius plots summarize key results and reveal possible connections in terms of`activation energies between hydrogen consumption and the amounts of different products. The total cycloalkane production and the consumption ofhydrogen both show a simple temperature dependence with the same{open_quote}activation{close_quote} energies. Methane production varies more rapidly with temperature but can still be described by a single exponential term. The final example, conversion to aromatics, displays a more complicated dependence with an accelerated yield at high temperature. This form of data analyses connects to a new routine for mass balance evaluations and it is now applied to model catalyst performance and to understand optimum reaction conditions. Other branches of this project include surface spectroscopic measurements of fresh, sulfided and used catalysts, characterization of partially hydrogenated naphthalenes and modeling of hydrogen activity at metal sulfides.
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| 7. |
- Hatzinikolaou, D.G, et al.
(författare)
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Production and partial characterisation of extracellular lipase from aspergillus niger
- 1996
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Ingår i: Biotechnology letters. - 0141-5492 .- 1573-6776. ; 18:5, s. 547-552
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Tidskriftsartikel (refereegranskat)abstract
- The production and certain kinetic characteristics of extracellular lipase from Aspergillus niger were investigated. It was possible to substantially enhance the activity of excreted lipase by optimising the interaction between carbon and nitrogen sources applying a two-parameter complete experimental design and response surface analysis. The enzyme was partially purified and a number of kinetic characteristics such as optimum pH and temperature, thermal and pH stability and K(m) were determined and discussed. The elevated levels of lipase activity (40.5 U/ml) found in this work competed favourably with most of those reported for lipase hyperproducing fungi.
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| 8. |
- Hirschauer, B., et al.
(författare)
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Large area synthesis of thin alumina films by laser ablation
- 1996
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Ingår i: Applied Surface Science. - 0169-4332 .- 1873-5584. ; 99:4, s. 285-291
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Tidskriftsartikel (refereegranskat)abstract
- Al2O3 has been ablated on commercially available 3″ silicon wafers at different distances between the target and the substrate and laser fluencies. ‘Amorphous' Al2O3 (γ-alumina with grain size <20 nm) was grown by pulsed laser deposition at room temperature. The structure, the morphology, the profile and the composition of the produced films have been investigated. Fully oxidised thin films (thickness ≤5 μm) with high uniformity and smoothness were synthesised without additional oxygen gas during the ablation. The quality of the films was independent of the ablation fluency and of the distance between target and substrate.
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| 9. |
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| 10. |
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