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- Abdollahi, Mehdi, 1985, et al.
(författare)
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Carboxymethyl cellulose-agar biocomposite film activated with summer savory essential oil as an antimicrobial agent
- 2019
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 126, s. 561-568
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Tidskriftsartikel (refereegranskat)abstract
- Carboxymethyl cellulose (CMC)-agar biocomposite film was developed by a solvent casting method and the effects of summer savory essential oil (SSEO) at 0.5, 1.0 and 1.5% v/v on antimicrobial, microstructural, mechanical and optical properties as well as water sensitivity of the films were studied. Results showed that incorporation of SSEO into the biocomposite film developed active films with good antimicrobial agent growth inhibition activity against Gram-positive bacteria (S. aureus, B. cereus and L. monocytogenes) and less powerfully against Gram-negative bacteria (E. coli). Addition of the SSEO at 1.0 and 1.5% increased microstructural heterogeneity of the films and hence significantly (p < 0.05) increased water vapor permeability of the films while reducing their tensile strength. In contrast, mechanical flexibility and surface hydrophobicity of the films was significantly (p < 0.05) improved as a function of SSEO addition. The active films showed substantially lower swelling ratio compared to the CMC-agar film when 1.5% SSEO was added, but the transparency of the films was reduced. Finally, the results showed that SSEO can act as an antimicrobial agent in combination with CMC-agar film. However, it modifies properties of the film depending on the applied concentration.
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| 6. |
- Amir, Mohd., et al.
(författare)
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Investigating architecture and structure-function relationships in cold shock DNA-binding domain family using structural genomics-based approach
- 2019
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Ingår i: International Journal of Biological Macromolecules. - : ELSEVIER SCIENCE BV. - 0141-8130 .- 1879-0003. ; 133, s. 484-494
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Tidskriftsartikel (refereegranskat)abstract
- Oligonucleotide/oligosaccharide-binding fold (OB-fold) plays a major role in the regulation of central dogma of life via binding though DNA and RNA. The OB-fold domains are diverse in nature and present in large number of proteins with verities of molecular functions. Here, we have investigated the distribution of sequence, structure and repeats of cold shock DNA-binding proteins (CSDB), a member of OB-fold, in all three kingdoms to establish functional relationships. The CSDB is consists of 30 domains with a major contribution of S1 (>110,601 sequences), S12 (>23,760 sequences), S17 (>14,833 sequences) and S28e (>1615 sequence) domains. These domains are largely found in bacteria (70-90%). The number of S1 domain repeats in eukaryota varies from 1 to 15 and are well-correlated with the protein size. The molecular function analysis suggests that a large number of repeats in the S1 domain are involved in diverse molecular functions in bacteria and eukaryotes. In-depth structure analysis of Si, S12, S17 and S28e domain-containing proteins of the OB-fold family provides a reasonable basis to understand the relationship of size and number of repeats with the corresponding molecular functions.
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| 7. |
- Bhattacharjee, Snehasish, et al.
(författare)
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Importance of the hydroxyl substituents in the B-ring of plant flavonols on their preferential binding interactions with VEGF G-quadruplex DNA : Multi-spectroscopic and molecular modeling studies
- 2018
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 118, s. 629-639
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Tidskriftsartikel (refereegranskat)abstract
- G-quadruplex (G4) structures are known to be promising anticancer drug targets and flavonols (an important class of fiavonoids) are small molecules reported to possess several health-promoting properties including those of anticancer activities. In this work, we explored the interactions of the structurally related plant flavonols kaempferol (KAE; 3,5,7,4'-OH flavone) and morin (MOR; 3,5,7,2',4'-OH flavone) with various G4-DNA sequences along with duplex DNA using a combination of spectroscopic and molecular docking studies. Our results revealed that KAE shows preferential interaction with VEGF G4-DNA in comparison to the other G4 sequences and duplex DNA. Moreover, KAE enhances the thermal stability of VEGF G4-DNA. In contrast, MOR exhibits an appreciably weaker level of interaction with both duplex and various G4-DNAs, with no significant structural specificity. The contrasting DNA binding behaviors suggest a crucial role of the 2'-OH substituent in the Bring of flavonol moiety. While KAE is relatively planar, MOR adopts a significantly non-planar conformation attributable to steric hindrance from the additional 2'-OH substituent. This small structural difference is apparently very important for the ability of KAE and MOR to interact with VEGF G4-DNA. Thus, KAE (but not MOR) appears to be an effective ligand for VEGF G4-DNA, opening up possibilities of its application for regulation of gene expression in cancer cells.
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| 8. |
- Castro Alves, Victor, 1986-, et al.
(författare)
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Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production
- 2017
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 95, s. 215-223
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Tidskriftsartikel (refereegranskat)abstract
- Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects. In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo-(MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages. Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as beta-(1,6)-branched beta-(1,3)-glucans, BaHW and MyEN as alpha-(1,3)-(1,2)-branched alpha-(1,6)-glucans, and BaHA as a mixture of alpha-(1,6) and beta-(1,3)-glucans. BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-alpha) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles. In contrast, BaHW and MyEN induced TNF-alpha, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides. In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable alpha- and beta-glucans with potential immunomodulatory effects.
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| 9. |
- Castro Alves, Victor, 1986-, et al.
(författare)
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Polysaccharides from chayote enhance lipid efflux and regulate NLRP3 inflammasome priming in macrophage-like THP-1 cells exposed to cholesterol crystals
- 2019
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 127, s. 502-510
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Tidskriftsartikel (refereegranskat)abstract
- The contribution of dietary fiber to decrease the risk of atherosclerosis may occur through other mechanisms besides the increased excretion of cholesterol. Although macrophages are crucial for lipid clearance, the excessive uptake of cholesterol crystals (CC) by these cells induce NLRP3 inflammasome and foam cell formation. Thus, we investigated whether the water-soluble DF from chayote (WSP) regulate CC-pretreated macrophage-like THP-1 cells. Linkage analysis indicated that WSP is composed mainly of pectic homogalacturonan and highly branched type I rhamnogalacturonan as well as hemicellulosic material including glucomannan, xyloglucan, and glucurono(arabino)xylan. WSP reduced interleukin (IL)-1β and chemokine release in CC-pretreated macrophages. Notably, WSP also reduced lipid accumulation in cells previously exposed to CC. Furthermore, WSP upregulated liver X receptor alpha expression, which may account for increased lipid efflux, and reduced matrix metallopeptidase 9 expression. WSP also reduced active caspase-1 protein levels, and downregulated NLRP3 and IL-1β gene expression in CC-pretreated cells, suggesting that this polysaccharide fraction regulates the priming signals required for NLRP3 inflammasome activation. Thus, WSP regulate lipid efflux and suppress inflammasome priming in macrophages, suggesting that the health benefits of this dietary fiber could go beyond its physical properties on the gastrointestinal tract.
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| 10. |
- Castro Alves, Victor, 1986-, et al.
(författare)
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α-and β-D-Glucans from the edible mushroom Pleurotus albidus differentially regulate lipid-induced inflammation and foam cell formation in human macrophage-like THP-1 cells
- 2018
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 111, s. 1222-1228
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Tidskriftsartikel (refereegranskat)abstract
- Macrophages play an essential role in lipid metabolism; however, the excessive uptake of modified lipids andcholesterol crystals (CC) leads to the formation of pro-inflammatory lipid-laden macrophages called foam cells.Since theα-1,6- andβ-1,3-D-glucans from the basidiome and the mycelium of the edible mushroomPleurotusalbidushave previously been shown to regulate macrophage function, these glucans were tested in macro-phage-like THP-1 cells previously exposed to acetylated low-density lipoproteins (acLDL) or CC. The glucansinhibited lipid-induced inflammation, but only theβ-1,3-D-glucan regulated both the NLRP3 inflammasome ac-tivation and the expression of genes involved on lipid efflux in acLDL- or CC-pretreated cells, thereby reducingfoam cell formation. In contrast, the twoα-1,6-glucans tested inhibited foam cell formation only in acLDL-pretreated cells and had no effect on the expression of the peroxisome proliferator-activated receptor gammaand liver X receptor alpha genes, suggesting that these glucans regulate lipid influx rather than lipid efflux.Thus,α-andβ-D-glucans differentially regulate lipid-induced inflammation and foam cell formation in macro-phage-like cells. Furthermore, results emphasize thatP. albidushas potential to be used as a functional food oras a source for the extraction of biologically-active glucans.
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