| 1. |
- Alallaq, Sanaria, 1976-
(author)
-
Characterization of adventitious root formation in Populus species and Norway spruce
- 2021
-
Doctoral thesis (other academic/artistic)abstract
- Adventitious root (AR) formation is a form of post-embryonic development and is a key adaptive trait in plants. De novo adventitious root regeneration represents an elegant evolutionary innovation that allows many plant species to multiply through vegetative propagation; it is widely used in forestry and horticulture to multiply elite genotypes. However, several tree species with high economic and ecological value are difficult to root, and the genetic and molecular bases underlying AR regeneration remain largely elusive. Recently our laboratory showed that jasmonate (JA) and cytokinins (CK) act cooperatively to repress AR initiation (ARI) in Arabidopsishypocotyls, while auxin positively controls ARI by repressing this negative effect. With the recent availability of the reference genomes of Populus spp. and Norway spruce (Picea abies), the aim of this thesis is to explore the molecular and mechanistic foundations of AR formation in woody species and check whether or not there is conservation of the molecular mechanisms identified in Arabidopsis. First, physiological, molecular and hormonic approaches coupled with extensive anatomical analysis were combined to explore the role of light spectral quality in the control of ARI in P. abies de-rooted seedlings. We showed that constant red light (cRL) promotes ARI by reducing the content of the wound-induced phytohormones JA and JA-isoleucine and repressing the accumulation of the isopentyl-adenine-type cytokinins. These results suggest that the cooperative role of JA and CK signaling in the repression of ARI is evolutionarily conserved.Next we compared transcriptomic data from the cambium tissue of woody stem cuttings of the hybrid aspen T89, which is difficult-to-root, and from the hybrid poplar OP42, which is easy-to-root, under hydroponic conditions. The analyses revealed high transcriptional activity in OP42, with twice as many transcription factors differentially expressed in OP42 24 hours after cutting compared to T89. Although we did not observe significant differences in the expression of Auxin response factor (ARF) genes between the two genotypes, the production of transgenic plants downregulating or over-expressing ARF6, 8 or 17 confirmed that PtARF6 and PtARF8 positively and PtARF17 negatively regulate AR development in transgenic hybrid aspen T89. Interestingly, the expression of MYC2 orthologs as well as the expression of several genes involved in JA signaling increased more in T89 than in OP42, suggesting that JA could be a negative regulator of ARI in Populus spp. We also showed that overexpressing PtMYC2 led to a reduced number of ARs in hybrid aspen T89 cuttings. In addition, many genes encoding ROS scavenging proteins such as peroxidases or GSTs were significantly differentially expressed in OP42 24 h after cutting but not in T89, which is interesting since peroxidase activity has often been positively correlated with ARI. In parallel to this research, we characterized the rooting phenotype of clones from the Swedish Aspen (SwAsp) collection usingin vitro cuttings. We observed a significant variation in the rooting ability as well as different root system establishment between the clones. We analyzed the expression of some genes known to be involved in AR development in selected clones with contrasting AR phenotypes but could not identify any correlation between gene expression and rooting phenotype. A transcriptomic analysis of selected clones, with contrasting AR phenotypes, could be a useful tool in the identification of marker genes, which can be used for future selection of the best rooting clones of Populus or other economically important trees in breeding programs.
|
|
| 2. |
- Alallaq, Sanaria, et al.
(author)
-
Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings
- 2020
-
In: Frontiers in Plant Science. - : Frontiers Media S.A.. - 1664-462X. ; 11
-
Journal article (peer-reviewed)abstract
- Vegetative propagation relies on the capacity of plants to regeneratede novoadventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. Here, we explored the role of red light (RL) on ARI in de-rooted Norway spruce seedlings. We combined investigation of hormone metabolism and gene expression analysis to identify potential signaling pathways. We also performed extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins. We demonstrated that exogenously applied JA and/or CK inhibit ARI in a dose-dependent manner and found that they possibly act in the same pathway. The negative effect of JA on ARI was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.
|
|
| 3. |
- Aubry, Emilie, et al.
(author)
-
A vacuolar hexose transport is required for xylem development in the inflorescence stem
- 2022
-
In: Plant Physiology. - : Oxford University Press. - 0032-0889 .- 1532-2548. ; 188:2, s. 1229-1247
-
Journal article (peer-reviewed)abstract
- In Angiosperms, the development of the vascular system is controlled by a complex network of transcription factors. However, how nutrient availability in the vascular cells affects their development remains to be addressed. At the cellular level, cytosolic sugar availability is regulated mainly by sugar exchanges at the tonoplast through active and/or facilitated transport. In Arabidopsis (Arabidopsis thaliana), among the genes encoding tonoplastic transporters, SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTER 16 (SWEET16) and SWEET17 expression has been previously detected in the vascular system. Here, using a reverse genetics approach, we propose that sugar exchanges at the tonoplast, regulated by SWEET16, are important for xylem cell division as revealed in particular by the decreased number of xylem cells in the swt16 mutant and the accumulation of SWEET16 at the procambium-xylem boundary. In addition, we demonstrate that transport of hexoses mediated by SWEET16 and/or SWEET17 is required to sustain the formation of the xylem secondary cell wall. This result is in line with a defect in the xylem cell wall composition as measured by Fourier-transformed infrared spectroscopy in the swt16swt17 double mutant and by upregulation of several genes involved in secondary cell wall synthesis. Our work therefore supports a model in which xylem development partially depends on the exchange of hexoses at the tonoplast of xylem-forming cells.
|
|
| 4. |
- Bannoud, Florencia, et al.
(author)
-
Adventitious Rooting in Populus Species : Update and Perspectives
- 2021
-
In: Frontiers in Plant Science. - : Frontiers Media S.A.. - 1664-462X. ; 12
-
Research review (peer-reviewed)abstract
- Populus spp. are among the most economically important species worldwide. These trees are used not only for wood and fiber production, but also in the rehabilitation of degraded lands. Since they are clonally propagated, the ability of stem cuttings to form adventitious roots is a critical point for plant establishment and survival in the field, and consequently for the forest industry. Adventitious rooting in different Populus clones has been an agronomic trait targeted in breeding programs for many years, and many factors have been identified that affect this quantitative trait. A huge variation in the rooting capacity has been observed among the species in the Populus genus, and the responses to some of the factors affecting this trait have been shown to be genotype-dependent. This review analyses similarities and differences between results obtained from studies examining the role of internal and external factors affecting rooting of Populus species cuttings. Since rooting is the most important requirement for stand establishment in clonally propagated species, understanding the physiological and genetic mechanisms that promote this trait is essential for successful commercial deployment.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Chardon, Fabien, et al.
(author)
-
Natural variation in the long-distance transport of nutrients and photoassimilates in response to N availability
- 2022
-
In: Journal of plant physiology (Print). - : Elsevier. - 0176-1617 .- 1618-1328. ; 273
-
Journal article (peer-reviewed)abstract
- Phloem and xylem tissues are necessary for the allocation of nutrients and photoassimilates. However, how the long-distance transport of carbon (C) and nitrogen (N) is coordinated with the central metabolism is largely unknown. To better understand how the genetic and environmental factors influence C and N transport, we analysed the metabolite profiles of phloem exudates and xylem saps of five Arabidopsis thaliana accessions grown in low or non-limiting N supply. We observed that xylem saps were composed of 46 or 56% carbohydrates, 27 or 45% amino acids, and 5 or 13% organic acids in low or non-limiting N supply, respectively. In contrast, phloem exudates were composed of 76 or 86% carbohydrates, 7 or 18% amino acids, and 5 or 6% organic acids. Variation in N supply impacted amino acid, organic acid and sugar contents. When comparing low N and non-limiting N, the most striking differences were variations of glutamine, aspartate, and succinate abundance in the xylem saps and citrate and fumarate abundance in phloem exudates. In addition, we observed a substantial variation of metabolite content between genotypes, particularly under high N. The content of several organic acids, such as malate, citrate, fumarate, and succinate was affected by the genotype alone or by the interaction between genotype and N supply. This study confirmed that the response of the transport of nutrients in the phloem and the xylem to N availability is associated with the regulation of the central metabolism and could be an adaptive trait.
|
|
| 8. |
- Dob, Asma, et al.
(author)
-
Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis
- 2021
-
In: Journal of Experimental Botany. - : Oxford University Press. - 0022-0957 .- 1460-2431. ; 72:20, s. 7107-7118
-
Journal article (peer-reviewed)abstract
- Adventitious rooting is a de novo organogenesis process that enables plants to propagate clonally and cope with environmental stresses. Adventitious root initiation (ARI) is controlled by interconnected transcriptional and hormonal networks, but there is little knowledge of the genetic and molecular programs orchestrating these networks. Thus, we have applied genome-wide transcriptome profiling to elucidate the transcriptional reprogramming events preceding ARI. These reprogramming events are associated with the down-regulation of cytokinin (CK) signaling and response genes, which could be triggers for ARI. Interestingly, we found that CK free base (iP, tZ, cZ, and DHZ) content declined during ARI, due to down-regulation of de novo CK biosynthesis and up-regulation of CK inactivation pathways. We also found that MYC2-dependent jasmonate (JA) signaling inhibits ARI by down-regulating the expression of the CYTOKININ OXIDASE/DEHYDROGENASE1 (CKX1) gene. We also demonstrated that JA and CK synergistically activate expression of the transcription factor RELATED to APETALA2.6 LIKE (RAP2.6L), and constitutive expression of this transcription factor strongly inhibits ARI. Collectively, our findings reveal that previously unknown genetic interactions between JA and CK play key roles in ARI.
|
|
| 9. |
- Kidwai, Maria, et al.
(author)
-
Species-specific transcriptional reprogramming during adventitious root initiation
- 2023
-
In: Trends in Plant Science. - : Elsevier. - 1360-1385 .- 1878-4372. ; 28:2, s. 128-130
-
Journal article (peer-reviewed)abstract
- Adventitious roots or shoot-borne roots transdifferentiate from cells close to vascular tissues after cell reprogramming, which is associated with increased transcriptional activity. Recently, Garg et al. provided a genome-wide landscape of transcriptional signatures during the early stages of adventitious root initiation in rice and showed that conserved transcription factors acquire species-specific function.
|
|
| 10. |
- Lakehal, Abdellah, et al.
(author)
-
ETHYLENE RESPONSE FACTOR 115 integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting in Arabidopsis
- 2020
-
In: New Phytologist. - : Wiley-Blackwell Publishing Inc.. - 0028-646X .- 1469-8137. ; 228, s. 1611-1626
-
Journal article (peer-reviewed)abstract
- Adventitious root initiation (ARI) is ade novoorganogenesis program and a key adaptive trait in plants. Several hormones regulate ARI but the underlying genetic architecture that integrates the hormonal crosstalk governing this process remains largely elusive. In this study, we use genetics, genome editing, transcriptomics, hormone profiling and cell biological approaches to demonstrate a crucial role played by the APETALA2/ETHYLENE RESPONSE FACTOR 115 transcription factor. We demonstrate that ERF115 functions as a repressor of ARI by activating the cytokinin (CK) signaling machinery. We also demonstrate thatERF115is transcriptionally activated by jasmonate (JA), an oxylipin-derived phytohormone, which represses ARI in NINJA-dependent and independent manners. Our data indicate that NINJA-dependent JA signaling in pericycle cells blocks early events of ARI. Altogether, our results reveal a previously unreported molecular network involving cooperative crosstalk between JA and CK machineries that represses ARI.
|
|
