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- Mieliauskiene, R, et al.
(författare)
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Amperometric determination of acetate with a tri-enzyme based sensor
- 2006
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Ingår i: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 113:2, s. 671-676
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Tidskriftsartikel (refereegranskat)abstract
- The present work describes a flow injection analysis system incorporating amperometric biosensors for acetate monitoring. Tri-enzyme-modified electrodes were fabricated by immobilizing acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) into a poly(ethyleneglycole) diglycidyl ether (PEGDGE) film also containing Brilliant Cresyl Blue (BCB) as an electrochemical mediator. The concentration of acetate could be determined from the decrease in the amperometric current at +50 mV versus Ag/AgCl. Parameters, such as composition of the biosensing film, operational conditions (e.g., applied potential, flow rate) were optimized. The optimized biosensor showed a linear response to acetate in the range of 0.2-8 mM with a detection limit of 0.13 mM (calculated as 3 SIN). The acetate content of different samples (wine and vinegar) could be reliably quantified with the described biosensor.
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