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- Barut, Inci, 1984, et al.
(author)
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Correlative Cellular Mass Spectrometry Imaging and Amperometry Show Dose Dependent Changes in Lipid Composition and Exocytosis
- 2023
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In: Angewandte Chemie - International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 62:15
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Journal article (peer-reviewed)abstract
- Aberrant functioning of the proteasome has been associated with crucial pathologic conditions including neurodegeneration. Yet, the complex underlying causes at the cellular level remain unclear and there are conflicting reports of neuroprotective to neurodegenerative effects of proteasomal inhibitors such as lactacystin that are utilised as models for neurodegenerative diseases. The conflicting results may be associated with different dose regimes of lactacystin and hence we have performed a dose dependent study of the effects of lactacystin to identify concurrent changes in the cell membrane lipid profile and the dynamics of exocytosis using a combination of surface sensitive mass spectrometry and single cell amperometry. Significant changes of negatively charged lipids were associated with different lactacystin doses that showed a weak correlation with exocytosis while changes in PE and PE−O lipids showed dose dependent changes correlated with initial pore formation and total release of vesicle content respectively.
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| 4. |
- Oskarsdotter, Kristin, 1995, et al.
(author)
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Autologous endothelialisation by the stromal vascular fraction on laminin-bioconjugated nanocellulose-alginate scaffolds
- 2023
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In: Biomedical Materials (Bristol). - 1748-605X .- 1748-6041. ; 18:4
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Journal article (peer-reviewed)abstract
- Establishing a vascular network in biofabricated tissue grafts is essential for ensuring graft survival. Such networks are dependent on the ability of the scaffold material to facilitate endothelial cell adhesion; however, the clinical translation potential of tissue-engineered scaffolds is hindered by the lack of available autologous sources of vascular cells. Here, we present a novel approach to achieving autologous endothelialisation in nanocellulose-based scaffolds by using adipose tissue-derived vascular cells on nanocellulose-based scaffolds. We used sodium periodate-mediated bioconjugation to covalently bind laminin to the scaffold surface and isolated the stromal vascular fraction and endothelial progenitor cells (EPCs; CD31+CD45−) from human lipoaspirate. Additionally, we assessed the adhesive capacity of scaffold bioconjugation in vitro using both adipose tissue-derived cell populations and human umbilical vein endothelial cells. The results showed that the bioconjugated scaffold exhibited remarkably higher cell viability and scaffold surface coverage by adhesion regardless of cell type, whereas control groups comprising cells on non-bioconjugated scaffolds exhibited minimal cell adhesion across all cell types. Furthermore, on culture day 3, EPCs seeded on laminin-bioconjugated scaffolds showed positive immunofluorescence staining for the endothelial markers CD31 and CD34, suggesting that the scaffolds promoted progenitor differentiation into mature endothelial cells. These findings present a possible strategy for generating autologous vasculature and thereby increase the clinical relevance of 3D-bioprinted nanocellulose-based constructs.
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| 5. |
- Oskarsdotter, Kristin, 1995, et al.
(author)
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Injectable In Situ Crosslinking Hydrogel for Autologous Fat Grafting
- 2023
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In: Gels. - 2310-2861. ; 9:10
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Journal article (peer-reviewed)abstract
- Autologous fat grafting is hampered by unpredictable outcomes due to high tissue resorption. Hydrogels based on enzymatically pretreated tunicate nanocellulose (ETC) and alginate (ALG) are biocompatible, safe, and present physiochemical properties capable of promoting cell survival. Here, we compared in situ and ex situ crosslinking of ETC/ALG hydrogels combined with lipoaspirate human adipose tissue (LAT) to generate an injectable formulation capable of retaining dimensional stability in vivo. We performed in situ crosslinking using two different approaches; inducing Ca2+ release from CaCO3 microparticles (CMPs) and physiologically available Ca2+ in vivo. Additionally, we generated ex situ-crosslinked, 3D-bioprinted hydrogel-fat grafts. We found that in vitro optimization generated a CMP-crosslinking system with comparable stiffness to ex situ-crosslinked gels. Comparison of outcomes following in vivo injection of each respective crosslinked hydrogel revealed that after 30 days, in situ crosslinking generated fat grafts with less shape retention than 3D-bioprinted constructs that had undergone ex situ crosslinking. However, CMP addition improved fat-cell distribution and cell survival relative to grafts dependent on physiological Ca2+ alone. These findings suggested that in situ crosslinking using CMP might promote the dimensional stability of injectable fat-hydrogel grafts, although 3D bioprinting with ex situ crosslinking more effectively ensured proper shape stability in vivo.
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| 6. |
- Rudin, Rebecka, et al.
(author)
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RePrint: Digital workflow for aesthetic retrofitting of deteriorated architectural elements with new biomaterial finishes
- 2023
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In: Hybrids and Haecceities - Proceedings of the 42nd Annual Conference of the Association for Computer Aided Design in Architecture, ACADIA 2022. ; , s. 336-345
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Conference paper (peer-reviewed)abstract
- Digital fabrication offers new opportunities for revitalizing aged buildings in the time of craft expertise decline and higher demands regarding the sustainability of employed materials. Precise reproduction of architectural elements with digital 3D reconstruction methods such as photo-grammetry, and their repair using agile robotic 3D printing involving new environmentally friendly materials can save time and resources, leading to more circular design and manufacturing. This study presents digital workflows for architectural restoration, based on the concept of aesthetic retrofitting of deteriorated wooden architectural elements through the application of surface finishes from a novel biomaterial – nanocellulose hydrogel, upcycled from forestry waste. The workflows were established through experimental digital design and reproduction of wooden architectural details in an existing historical building, and executed within an integrated digital framework combining photogrammetry, 2D graphics processing, computational design and robotic 3D printing. Overall, the investigation has sought to demonstrate the potential of nanocellulose as a material suitable for applications in renovation and conservation. Further, the intention was to elucidate the role of digital tools as new media of restoration that enable to uplift cultural assets in an alterna-tive way - by allowing to embed aesthetic features conveying the contemporaneity of remedial interventions. Aiming to contribute to current work in experimental preservation, the study offers a novel approach in which deteriorated architectural elements are endowed with a new materiality that follows the new logic of circularity in contemporary design and construction.
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| 7. |
- Zboinska, Malgorzata, 1981, et al.
(author)
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Robotically 3D printed architectural membranes from ambient dried cellulose nanofibril-alginate hydrogel
- 2023
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In: Materials and Design. - 1873-4197 .- 0264-1275. ; 236
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Journal article (peer-reviewed)abstract
- Cellulose nanofibril hydrogel mixed with an aqueous solution of sodium alginate is a novel bio-based material suitable for 3D printing of lightweight membranes with exquisite properties and sustainable traits. However, fun- damental knowledge enabling its applications in architectural design is still missing. Hence, this study examines the macro-scale features of lightweight membranes from cellulose nanofibril-alginate hydrogel, relevant for the design of various interior architectural products, such as wall claddings, ceiling tiles, room partitions, tapestries, and window screens. Through iterative prototyping experiments involving robotic 3D printing of lightweight membranes, their upscaling potential is demonstrated. Correlations between toolpath designs and shrinkages are also characterized, alongside an in-depth analysis of coloration changes upon ambient drying. Further, the tunability potential of various architectural features, enabled by bespoke 3D printing toolpath design, is discussed and exemplified. The aim is to expose the wide palette of design possibilities for cellulose nanofibril-alginate membranes, encompassing variations in curvature, porosity, translucency, texture, patterning, pliability, and feature sizes. The results comprise an important knowledge foundation for the design and manufacturing of custom lightweight architectural products from cellulose nanofibril-alginate hydrogel. These products could be applied in a variety of new bio-based, sustainable interior building systems, replacing environmentally harmful, fossil-based solutions.
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