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- Fyrner, Timmy
(författare)
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Synthesis of Orthogonally Functionalized Oligosaccharides for Self-assembled Monolayers and as Multimodal Tools in Chemical Biology
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis covers different topics in the field of synthetic organic chemistry combined with the field of surface science and glycobiology.First, the text presents a series of orthogonally protected oligosaccharides (tri-, penta-, and heptasaccharides) of varying length and structures, which are synthesized with the aim of developing novel heterobifunctional biocompatible cross-linkers. Successful conjugation with different chemical handles is also described and used to illustrate the potential implementation of defined carbohydrate based compounds have potential use in biosensing applications. The results of incubation experiments using living cells indicate that the linker is incorporated into cell surfaces and enriched in microdomains.Second, synthesis of various saccharide-terminated alkane thiols immobilized on gold surfaces is reported. The protein adsorption and antifouling characteristics of these surfaces were investigated using model proteins and the common fouling organisms, Ulva linza and Balanus amphitrite.Further, oligo(lactose)-based thiols (di-, tetra-, and hexasaccharides) were synthesized and immobilized on gold nanoparticles to investigate how well these rigid, rod-like oligosaccharides can stabilize such nanoparticles for future use in constructing hybrid nanoparticles.Finally, the thesis describes synthesis of a systematic series of oligo(ethylene) glycols possessing either hydrogen- or methyl-terminated groups. Investigation of the fundamental characteristics of self-assembled monolayers, will give important insights into the design of protein repellant surfaces.
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| 2. |
- Nilsson, Stefan, 1975-
(författare)
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Exploring the relationship between awareness information and user activities online
- 2010
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis contributes to the domain of awareness information. Awareness information can be viewed as a fundamental building-block of social media, visualizing people and their activities in an online setting. With life becoming more and more influenced by social media such as Facebook, Flickr, Twitter, Linkedin and Digg, it becomes important for designers of social media to have an understanding of how users respond to awareness information. An online gallery of pictures was developed and equipped with a chat and a basic awareness information system. Usage data was collected by the system and analyzed combining qualitative as well as quantitative approaches aiming to explore the influence awareness information have on the usage of the system. The analysis reveals that awareness information is a powerful tool triggering social behaviour. It is, at the same time, challenging to implement in a way that it provides a sound environment for social interaction. This was manifested by co-present users both stayed longer in the system and also was inclined to take a different path through the gallery than the solitary user. However, the interaction that took place in the gallery was characterized by frequent conversational break-downs and irritation among users. Results indicate that this is, in part, due to the lack of built-in social norms guiding users in their activities in the system. Subsequently, it is important to facilitate the creation, recreation and reinforcement of social norms when designing social systems.
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