| 1. |
- Rohrbach, Daniel J., et al.
(författare)
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Characterization of nonmelanoma skin cancer for light therapy using spatial frequency domain imaging
- 2015
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Ingår i: Biomedical Optics Express. - : Optical Society of America. - 2156-7085. ; 6:5, s. 1761-1766
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Tidskriftsartikel (refereegranskat)abstract
- The dosimetry of light-based therapies critically depends on both optical and vascular parameters. We utilized spatial frequency domain imaging to quantify optical and vascular parameters, as well as estimated light penetration depth from 17 nonmelanoma skin cancer patients. Our data indicates that there exist substantial spatial variations in these parameters. Characterization of these parameters may inform understanding and optimization of the clinical response of light-based therapies.
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| 2. |
- Saager, Rolf B., 1974-, et al.
(författare)
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From theory to practice : the broadening role of polydimethylsiloxane phantoms as an intermediary between model validation and instrument performance testing (Conference Presentation)
- 2016
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Ingår i: Proceedings Volume 9700, Design and Quality for Biomedical Technologies IX; 97000G (2016). - : SPIE - International Society for Optical Engineering.
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Konferensbidrag (refereegranskat)abstract
- Polydimethylsiloxane (PDMS) has been a popular medium to fabricate tissue simulating optical phantoms. Recently, its use has significantly expanded in instrument calibration and performance testing, validation of advanced models of light transport of complex tissue geometries and evaluation of novel measurement modalities. To meet these demands, fabrication methods of these optical phantoms have become more refined and its structure and constituent components (i.e. dyes and scattering agents) have evolved to better mimic optical properties of tissue spanning both visible and near infrared regimes. We present efforts at the Beckman Laser Institute that address these challenges through PDMS phantoms.
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| 3. |
- Saager, Rolf B., 1974-, et al.
(författare)
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In vivo measurements of cutaneous melanin across spatial scales : using multiphoton microscopy and spatial frequency domain spectroscopy
- 2015
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Ingår i: Journal of Biomedical Optics. - : SPIE - International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 20:6
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Tidskriftsartikel (refereegranskat)abstract
- The combined use of nonlinear optical microscopy and broadband reflectance techniques to assess melanin concentration and distribution thickness in vivo over the full range of Fitzpatrick skin types is presented. Twelve patients were measured using multiphoton microscopy (MPM) and spatial frequency domain spectroscopy (SFDS) on both dorsal forearm and volar arm, which are generally sun-exposed and non-sun-exposed areas, respectively. Both MPM and SFDS measured melanin volume fractions between ∼5% (skin type I non-sun-exposed) and 20% (skin type VI sun exposed). MPM measured epidermal (anatomical) thickness values ∼30–65 μm, while SFDS measured melanin distribution thickness based on diffuse optical path length. There was a strong correlation between melanin concentration and melanin distribution (epidermal) thickness measurements obtained using the two techniques. While SFDS does not have the ability to match the spatial resolution of MPM, this study demonstrates that melanin content as quantified using SFDS is linearly correlated with epidermal melanin as measured using MPM (R2=0.8895). SFDS melanin distribution thickness is correlated to MPM values (R2=0.8131). These techniques can be used individually and/or in combination to advance our understanding and guide therapies for pigmentation-related conditions as well as light-based treatments across a full range of skin types.
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| 4. |
- Torabzadeh, Mohammad, et al.
(författare)
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hyperspectral characterization of tissue simulating phantoms using a supercontinuum laser in a spatial frequency domain imaging instrument
- 2018
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Ingår i: Proceedings Volume 10486, Design and Quality for Biomedical Technologies XI; 104860G (2018). - : SPIE - International Society for Optical Engineering.
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Konferensbidrag (refereegranskat)abstract
- Hyperspectral Imaging (HSI) is a growing field in tissue optics due to its ability to collect continuous spectral features of a sample without a contact probe. Spatial Frequency Domain Imaging (SFDI) is a non-contact wide-field spectral imaging technique that is used to quantitatively characterize tissue structure and chromophore concentration. In this study, we designed a Hyperspectral SFDI (H-SFDI) instrument which integrated a supercontinuum laser source to a wavelength tuning optical configuration and a sCMOS camera to extract spatial (Field of View: 2cm×2cm) and broadband spectral features (580nm-950nm). A preliminary experiment was also performed to integrate the hyperspectral projection unit to a compressed single pixel camera and Light Labeling (LiLa) technique.
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