| 1. |
- Angsten, G., et al.
(författare)
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Reference ranges for muscle carnitine concentration in children
- 2003
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Ingår i: Annals of Clinical Biochemistry. - : SAGE Publications. - 0004-5632 .- 1758-1001. ; 40:4, s. 406-410
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Tidskriftsartikel (refereegranskat)abstract
- Background: We investigated muscle and plasma carnitine concentrations in children to establish reference intervals for use following biopsy of skeletal muscle. Methods: The study comprised 50 children from newborns up to 14 years of age, all undergoing elective surgery. They were divided into six age groups, the youngest 0-2 days and the oldest 11-14 years. The samples were taken at the beginning of surgery. Results: Gestational age was a major determinant of the total muscle carnitine concentration in newborns (Spearman's rs= 0.692, P<0.01). This concentration was low during the first year, but subsequently did not differ between age groups. In neonates the median value (range) for total carnitine concentration in skeletal muscle was 5.9 (2.2-15.9) µmol/g dry weight and the free to total carnitine ratio was 62 (31-81)%. In children 1-12 months old the corresponding figures were 6.0 (3.5-7.9) µmol/g dry weight and 51 (28-71)% and in those 1-14 years they were 12.1 (6.6-17.4) µmol/g dry weight and 76 (42-92)%. Conclusion: This study shows that muscle carnitine concentrations in newborns are dependent on gestational age. The data suggest that there is an accretion of carnitine in skeletal muscle during the first year of life. Reference intervals are given.
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| 2. |
- Beck, O, et al.
(författare)
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Frontline immunochromatographic device for on-site urine testing of amphetamines: laboratory validation using authentic specimens
- 2000
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Ingår i: Annals of clinical biochemistry. - : SAGE Publications. - 0004-5632. ; 3737 ( Pt 2), s. 199-204
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Tidskriftsartikel (refereegranskat)abstract
- We evaluated a new test device for amphetamines and methamphetamines (Frontline ®, cut-off limit 300 ng/mL) using authentic clinical and forensic specimens. The device is based on immunochromatography and is dipped into urine and read visually by comparison with a colour scale after a few minutes. A total of 658 specimens were tested by comparing results of the screening procedure with established immunoassays. Discordant results were further investigated by gas chromatography- mass spectrometry or gas chromatography (with flame ionization detector). The Frontline device had a sensitivity of 93% and a specificity of 98%. When specimens were classified by urine amphetamine concentration, close agreement was obtained at concentrations below 150 ng/mL and above 1000 ng/mL. A small number of specimens with amphetamine concentrations between 300 and 1000 ng/mL tested negative in the Frontline test. This finding could to some extent be explained by the enantioselectivity of the antibodies in the Frontline test to d-amphetamine. We conclude that the performance of the Frontline test device for amphetamines is adequate for presumptive clinical and forensic screening.
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| 3. |
- Berry, D, et al.
(författare)
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New antiepileptic drugs
- 2000
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Ingår i: Annals of clinical biochemistry. - 0004-5632. ; 3737 ( Pt 4), s. 551-553
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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