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Träfflista för sökning "WFRF:(James Daniel) srt2:(2000-2004)"

Sökning: WFRF:(James Daniel) > (2000-2004)

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  • Park, Jiwoong, et al. (författare)
  • Wiring up single molecules
  • 2003
  • Ingår i: Thin Solid Films. - 0040-6090 .- 1879-2731. ; 438-439, s. 457-461
  • Tidskriftsartikel (refereegranskat)abstract
    • The possibility of using single molecules as active elements of electronic devices offers a variety of scientific and technological opportunities. In this article, we discuss transistors, where electrons flow through discrete quantum states of a single molecule. First, we will describe molecules, where current flows through one cobalt atom surrounded by two insulating terpyridyl ligands. Depending on the length of the insulating part of the molecules, two different behaviors are observed: Coulomb blockade for a longer molecule and the Kondo effect for a shorter molecule. We will also discuss measurements of the C70 fullerene and its dimer (C140). In C140 devices, the transport measurements are affected by an intercage vibrational mode that has an energy of 11 meV. We observe a large current increase when this mode is excited, indicating a strong coupling between the electronic and mechanical degrees of freedom in C140 molecules.
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  • Sigvardsson, Mikael, et al. (författare)
  • Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.
  • 2002
  • Ingår i: Molecular and Cellular Biology. - 0270-7306. ; 22:24, s. 8539-8551
  • Tidskriftsartikel (refereegranskat)abstract
    • Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development.
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