| 1. |
- Kraus, Robert H. S., et al.
(författare)
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Genome wide SNP discovery, analysis and evaluation in mallard (Anas platyrhynchos)
- 2011
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Ingår i: BMC Genomics. - : BioMed Central Ltd.. - 1471-2164. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Background: Next generation sequencing technologies allow to obtain at low cost the genomic sequence information that currently lacks for most economically and ecologically important organisms. For the mallard duck genomic data is limited. The mallard is, besides a species of large agricultural and societal importance, also the focal species when it comes to long distance dispersal of Avian Influenza. For large scale identification of SNPs we performed Illumina sequencing of wild mallard DNA and compared our data with ongoing genome and EST sequencing of domesticated conspecifics. This is the first study of its kind for waterfowl. Results: More than one billion base pairs of sequence information were generated resulting in a 16x coverage of a reduced representation library of the mallard genome. Sequence reads were aligned to a draft domesticated duck reference genome and allowed for the detection of over 122,000 SNPs within our mallard sequence dataset. In addition, almost 62,000 nucleotide positions on the domesticated duck reference showed a different nucleotide compared to wild mallard. Approximately 20,000 SNPs identified within our data were shared with SNPs identified in the sequenced domestic duck or in EST sequencing projects. The shared SNPs were considered to be highly reliable and were used to benchmark non-shared SNPs for quality. Genotyping of a representative sample of 364 SNPs resulted in a SNP conversion rate of 99.7%. The correlation of the minor allele count and observed minor allele frequency in the SNP discovery pool was 0.72. Conclusion: We identified almost 150,000 SNPs in wild mallards that will likely yield good results in genotyping. Of these, similar to 101,000 SNPs were detected within our wild mallard sequences and similar to 49,000 were detected between wild and domesticated duck data. In the similar to 101,000 SNPs we found a subset of similar to 20,000 SNPs shared between wild mallards and the sequenced domesticated duck suggesting a low genetic divergence. Comparison of quality metrics between the total SNP set (122,000 + 62,000 = 184,000 SNPs) and the validated subset shows similar characteristics for both sets. This indicates that we have detected a large amount (similar to 150,000) of accurately inferred mallard SNPs, which will benefit bird evolutionary studies, ecological studies (e. g. disentangling migratory connectivity) and industrial breeding programs.
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| 2. |
- Lakhan, Abdullah, et al.
(författare)
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Dynamic application partitioning and task-scheduling secure schemes for biosensor healthcare workload in mobile edge cloud
- 2021
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Ingår i: Electronics. - : MDPI AG. - 2079-9292. ; 10:22, s. 1-30
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Tidskriftsartikel (refereegranskat)abstract
- Currently, the use of biosensor-enabled mobile healthcare workflow applications in mobile edge-cloud-enabled systems is increasing progressively. These applications are heavyweight and divided between a thin client mobile device and a thick server edge cloud for execution. Application partitioning is a mechanism in which applications are divided based on resource and energy parameters. However, existing application-partitioning schemes widely ignore security aspects for healthcare applications. This study devises a dynamic application-partitioning workload task-scheduling-secure (DAPWTS) algorithm framework that consists of different schemes, such as min-cut algorithm, searching node, energy-enabled scheduling, failure scheduling, and security schemes. The goal is to minimize the energy consumption of nodes and divide the application between local nodes and edge nodes by applying the secure min-cut algorithm. Furthermore, the study devises the secure-min-cut algorithm, which aims to migrate data between nodesin a secure form during application partitioning in the system. After partitioning the applications, the node-search algorithm searches optimally to run applications under their deadlines. The energy and failure schemes maintain the energy consumption of the nodes and the failure of the system. Simulation results show that DAPWTS outperforms existing baseline approaches by 30% in terms of energy consumption, deadline, and failure of applications in the system.
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| 3. |
- Li, Hezun, et al.
(författare)
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Corporate governance and innovation
- 2019
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Ingår i: A research agenda for entrepreneurship and innovation. - Cheltenham : Edward Elgar Publishing. - 978 1 78811 600 8
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Bokkapitel (populärvet., debatt m.m.)
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| 4. |
- Li, Hezun, et al.
(författare)
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Corporate governance in entrepreneurial firms : a systematic review and research agenda
- 2020
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Ingår i: Small Business Economics. - : Springer. - 0921-898X .- 1573-0913. ; 54:1, s. 43-74
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Forskningsöversikt (refereegranskat)abstract
- This systematic review covers the extant literature on corporate governance in entrepreneurial firms. Using a sample of 137 research papers published from pre-1990 through June 2018 in 60 journals, we categorize outlets, research methods (quantitative, qualitative, review, and non-empirical), theoretical perspectives, and research questions, highlighting key patterns. We then summarize the concepts under study in the sample literature, and the geographical sources and model specification of quantitative empirical studies. The conclusion highlights the quite fragmented nature of the field and the substantial knowledge gaps, and then proposes an actionable agenda for future research in terms of theories, research questions, research settings, and research designs. In particular, we describe the need to explore how corporate governance mechanisms interact with one another and affect firm outcomes, by applying novel theoretical perspectives and methods that could provide a better understanding of entrepreneurial firms' functioning.
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| 5. |
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| 6. |
- Oweson, Carolina A. M., 1976, et al.
(författare)
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Effects of manganese and hypoxia on coelomocyte renewal in the echinoderm, Asterias rubens (L.)
- 2010
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Ingår i: Aquatic Toxicology. - : Elsevier BV. - 0166-445X .- 1879-1514. ; 100:1, s. 84-90
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Tidskriftsartikel (refereegranskat)abstract
- Manganese (Mn) is a naturally abundant metal and particularly so in soft-bottom oceanic sediments where it generally occurs bound in a four-valent colloidal state as MnO2. When hypoxic conditions occur in bottom waters, the metal reduces to the bioavailable ion Mn2+ and can reach concentrations known to have immunotoxic effects in the crustacean Nephrops norvegicus, reducing numbers of circulating haemocytes as a consequence. However, we have previously shown that Mn seems to have a contrasting effect on the echinoderm Asterias rubens in which it triggers the proliferation of haematopoietic cells and increases coelomocyte numbers. Since elevated Mn levels mostly co-occur with hypoxia in nature, here we investigated whether hypoxia has a negative effect on haematopoiesis. Proliferation and differentiation of coelomocytes and cells in the coelomic epithelium of A. rubens were compared after 3 days of exposure to realistic levels of Mn, hypoxia or a combination of these two parameters. We can confirm that Mn elevated numbers of coelomocytes and increased proliferation of epithelial cells, but hypoxia did not affect these levels. However, hypoxia did affect differentiation of these cells as judged by investigating the expression of a Runt domain transcription factor, which was also cloned and sequenced. Through comparative quantification using a real time PCR technique, we found that exposure to hypoxia had a clearly stimulating effect on mRNA expression of Runt gene in both coelomocytes and epithelial cells. These results indicate that during hypoxic conditions the composition of coelomocyte sub-populations changed. (c) 2010 Elsevier B.V. All rights reserved.
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| 7. |
- Rolandsson Enes, Sara, et al.
(författare)
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Primary mesenchymal stem cells in human transplanted lungs are CD90/CD105 perivascularly located tissue-resident cells
- 2014
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Ingår i: BMJ Open Respiratory Research. - : BMJ Publishing Group. - 2052-4439. ; 1:1
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Mesenchymal stem cells (MSC) have not only been implicated in the development of lung diseases, but they have also been proposed as a future cell-based therapy for lung diseases. However, the cellular identity of the primary MSC in human lung tissues has not yet been reported. This study therefore aimed to identify and characterise the ‘bona fide’ MSC in human lungs and to investigate if the MSC numbers correlate with the development of bronchiolitis obliterans syndrome in lung-transplanted patients. METHODS: Primary lung MSC were directly isolated or culture-derived from central and peripheral transbronchial biopsies of lung-transplanted patients and evaluated using a comprehensive panel of in vitro and in vivo assays. RESULTS: Primary MSC were enriched in the CD90/CD105 mononuclear cell fraction with mesenchymal progenitor frequencies of up to four colony-forming units, fibroblast/100 cells. In situ staining of lung tissues revealed that CD90/CD105 MSCs were located perivascularly. MSC were tissue-resident and exclusively donor lung-derived even in biopsies obtained from patients as long as 16 years after transplantation. Culture-derived mesenchymal stromal cells showed typical in vitro MSC properties; however, xenotransplantation into non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice showed that lung MSC readily differentiated into adipocytes and stromal tissues, but lacked significant in vivo bone formation. CONCLUSIONS: These data clearly demonstrate that primary MSC in human lung tissues are not only tissue resident but also tissue-specific. The identification and phenotypic characterisation of primary lung MSC is an important first step in identifying the role of MSC in normal lung physiology and pulmonary diseases.
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| 8. |
- Wulff Hamrin, Cornelia, et al.
(författare)
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Svenska kyrkan och äldres välbefinnande : betydelsen av en församlings öppna verksamheter
- 2022
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Ingår i: äic Vetenskapligt supplement. - : Stiftelsen Äldrecentrum. - 2003-9069. ; 2:1, s. 29-41
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Tidskriftsartikel (refereegranskat)abstract
- Äldre personers välbefinnande, och hur kyrkan och dess församlingar genom sina öppna verksamheter kan bidra till detta välbefinnande, står i fokus för denna studie. Vi genomförde tretton intervjuer med personer över 65 år som besökt någon öppen verksamhet i en församling i en stad i Mellansverige sommaren 2021. En induktiv tematisk analys resulterade i fem teman: kontakt med andra, fysisk aktivitet, sysselsättning, kyrkan och församlingen samt platser att gå till. Resultatet visar betydelsen av dessa faktorer för att minska känslan av ensamhet och bryta isolering. Det överensstämmer med tidigare forskning, men pekar också på kyrkans och församlingens potentiella betydelse för att ge äldre personer sammanhang och sysselsättning. Det finns få studier som har undersökt detta och mer forskning behövs. Det behövs också fler sociala aktiviteter och träffpunkter för äldre i Sverige.
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