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Composite islet-end...
Composite islet-endothelial cell grafts a novel approach to counteract innate immunity in islet transplantation
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Johansson, Ulrika 1974- (författare)
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Elgue, Graciela (författare)
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Nilsson, Bo (författare)
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visa fler...
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Korsgren, Olle (författare)
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visa färre...
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(utgivare)
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(medarbetare)
- 2005
- 2005
- Engelska.
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Ingår i: American Journal of Transplantation. - 1600-6135. ; 5:11, 2632-2639
Abstract
Ämnesord
Stäng
- An instant blood-mediated inflammatory reaction (IBMIR) is elicited when islets come in contact with blood after intraportal transplantation. In contrast, endothelial cells (EC) readily tolerate contact with blood. A conceivable strategy to overcome IBMIR would be to create composite islet-EC grafts. Human islets were co-cultured with primary human aortic endothelial cells (HAEC) for 2-7 days to obtain 50-90% coverage. HAEC-coated islets were exposed to ABO-identical blood and analyzed with regard to clotting time, signs of inflammation and cell infiltration. Composite islet-HAEC graft survival was assessed after transplantation to athymic (nu/nu) nude mice. Exposed to blood, HAEC-coated islets induced less activation of coagulation and complement compared to control islets. Also, platelet and leukocyte consumption in blood was decreased. Clots with entrapped HAEC-coated islets showed less infiltration of CD11b+ cells. The extent of protection correlated to the level of HAEC coverage. Transplanted composite grafts stained positive for insulin and PECAM-1 demonstrating presence of both islets and HAEC within the islet graft 7 weeks after transplantation. Composite islet-HAEC grafts reduce all components of IBMIR. Refinement of the technique will allow introduction of composite islet-EC grafts in clinical islet transplantation, using autologous EC expanded in vitro and kept frozen until allogeneic islets become available for that specific recipient.
Ämnesord
- Medical and Health Sciences (hsv)
- Medicin och hälsovetenskap (hsv)
- MEDICINE (svep)
- MEDICIN (svep)
Nyckelord
- Endothelial Cells
- transplantation
- islets of Langerhans
- gratf survival
- immunology
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