Search: onr:"swepub:oai:DiVA.org:his-11408" >
Elastogenesis in hu...
Elastogenesis in human arterial disease : A role for macrophages in disordered elastin synthesis
-
- Krettek, Alexandra, 1968- (author)
- Brigham and Women's Hospital, Harvard Medical School, Boston, United States
-
- Sukhova, Galina K. (author)
- Brigham and Women's Hospital, Harvard Medical School, Boston, United States
-
- Libby, Peter (author)
- Brigham and Women's Hospital, Harvard Medical School, Boston, United States
-
(creator_code:org_t)
- Lippincott Williams & Wilkins, 2003
- 2003
- English.
-
In: Arteriosclerosis, Thrombosis and Vascular Biology. - : Lippincott Williams & Wilkins. - 1079-5642 .- 1524-4636. ; 23:4, s. 582-587
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- OBJECTIVE: Elastin, an extracellular matrix protein, constitutes about 30% of the dry weight of the arteries. Elastolysis induced by inflammatory processes is active in chronic arterial diseases. However, elastogenesis in arterial diseases has received little attention. In this work we hypothesized that disordered elastogenesis is active in matrix remodeling in atheroma and abdominal aortic aneurysm (AAA).METHODS AND RESULTS: Human AAA and atheroma have 4- to 6-fold more tropoelastin protein than nondiseased arteries. The smooth muscle cell-containing media and fibrous cap of atherosclerotic arteries contain ordered mature elastin, whereas macrophage (MPhi)-rich regions often have disorganized elastic fibers. Surprisingly, in addition to smooth muscle cells, MPhis in diseased arteries also produce the elastin precursor tropoelastin, as shown by double immunostaining, in situ hybridization, and reverse transcription-polymerase chain reaction for tropoelastin mRNA. Cultured monocyte-derived MPhis can express the elastin gene. AAA have 9-fold but atheroma only 1.6-fold lower levels of desmosine, a marker for mature cross-linked elastin, than normal arteries.CONCLUSIONS: This study demonstrates ongoing but often ineffective elastogenesis in arterial disease and establishes human macrophages as a novel source for this important matrix protein. These results have considerable import for understanding mechanisms of extracellular matrix remodeling in arterial diseases.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- AAA
- Atherosclerosis
- Elastin
- Macrophages
- Medical sciences
- Medicin
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database