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Evaluation of trans...
Evaluation of transcriptional biomarkers using a high-resolution regression approach : Concentration-dependence of selected transcripts in copper-exposed freshwater mussels (Anodonta anatina)
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- Ekelund Ugge, Gustaf Magnus Oskar (författare)
- University of Skövde,Lund University,Lunds universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Department of Biology, Lund University, Sweden,Ekologisk modellering, Ecological Modeling,Enhet akvatisk ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Akvatisk ekologi,Forskargrupper vid Lunds universitet,Division aquatic ecology,Faculty of Science,Aquatic Ecology,Lund University Research Groups
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- Jonsson, Annie (författare)
- University of Skövde,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Ekologisk modellering, Ecological Modeling
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- Walstad, Anders (författare)
- ALS Scandinavia Toxicon AB, Härslöv, Sweden
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- Berglund, Olof (författare)
- Lund University,Lunds universitet,Enhet akvatisk ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Akvatisk ekologi,Forskargrupper vid Lunds universitet,Division aquatic ecology,Department of Biology,Faculty of Science,Aquatic Ecology,Lund University Research Groups
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(creator_code:org_t)
- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: Environmental Toxicology and Pharmacology. - : Elsevier. - 1382-6689 .- 1872-7077. ; 90
- Relaterad länk:
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https://doi.org/10.1...
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https://his.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- We tested concentration-dependence of selected gene transcripts (cat, gst, hsp70, hsp90, mt and sod) for evaluation as biomarkers of chemical stress. Contrary to the common approach of factorial designs and few exposure concentrations, we used regression across a high-resolution concentration series. Specifically, freshwater mussels (Anodonta anatina) were acutely (96 h) exposed to Cu (13 nominal concentrations, measuring 0.13–1 600 µg/L), and transcripts were measured by RT-qPCR. In digestive glands, cat, hsp90 and mt decreased with water Cu (p < 0.05), but response magnitudes saturated at < 2-fold decreases. In gills, gst, hsp70, hsp90 and mt increased with water Cu (p < 0.05). While hsp70, hsp90 and mt exceeded 2-fold increases within the exposure range, high Cu concentrations were required (38–160 µg/L). Although gill responses were generally more robust compared to digestive glands, overall small response magnitudes and moderate sensitivity may set limit for potential application as general biomarkers of chemical stress.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Annan biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Other Biological Topics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Bivalves
- Dose-response
- Molecular biomarkers
- Response magnitude
- RT-qPCR
- Unionida
- Ekologisk modellering
- Ecological Modelling Group
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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